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| 1 | 生物医学影像中的光声成像技术显示文摘光声成像结合了光学成像的高对比度和超声成像的深穿透性优势,能够利用内源性、外源性造影剂对比显示组织的结构、功能、代谢特征和分子、动力学信息等,同时可以实现从细胞器、细胞、组织到器官的多尺度成像,在生物医学研究中发挥着越来越重要的作用。简要回顾了光声成像的基本原理,重点总结了光声计算断层成像(PACT)、光声显微成像(PAM)、光声内窥成像(PAE)和光声分子成像近年来的研究热点及技术进展,主要涉及成像探测方式的选择与改进、低成本激发光源的替代方案、图像重建算法的进步、系统成像速度和分辨率的提高以及分子探针的新兴设计策略等,最后展望了光声成像的应用前景。 | 穆根 张振辉 石玉娇 | 2022 | 中国激光2022，49，20： | 6 |
| 2 | Multifocal structured illumination optoacoustic microscopy显示文摘Optoacoustic(OA)imaging has the capacity to effectively bridge the gap between macroscopic and microscopic realms in biological imaging.High-resolution OA microscopy has so far been performed via point-by-point scanning with a focused laser beam,thus greatly restricting the achievable imaging speed and/or field of view.Herein we introduce multifocal structured illumination OA microscopy(MSIOAM)that attains real-time 3D imaging speeds.For this purpose,the excitation laser beam is shaped to a grid of focused spots at the tissue surface by means of a beamsplitting diffraction grating and a condenser and is then scanned with an acousto-optic deflector operating at kHz rates.In both phantom and in vivo mouse experiments,a 10mm wide volumetric field of view was imaged with 15 Hz frame rate at 28μm spatial resolution.The proposed method is expected to greatly aid in biological investigations of dynamic functional,kinetic,and metabolic processes across multiple scales. | Zhenyue Chen Ali Ozbek Johannes Rebling Quanyu Zhou Xose Luis Dean-Ben Daniel Razansky | 2020 | Light(Science & Applications)2020，9，1： | 0 |
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