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| 1 | Single-particle trapping and dynamic manipulation with holographic optical surface-wave tweezers显示文摘Optical surface waves have widely been used in optical tweezers systems for trapping particles sized from the nanoto microscale,with specific importance and needs in applications of super-resolved detection and imaging if a single particle can be trapped and manipulated accurately.However,it is difficult to achieve such trapping with high precision in conventional optical surface-wave tweezers.Here,we propose and experimentally demonstrate a new method to accurately trap and dynamically manipulate a single particle or a desired number of particles in holographic optical surface-wave tweezers.By tailoring the optical potential wells formed by surface waves,we achieved trapping of the targeted single particle while pushing away all surrounding particles and further dynamically controlling the particle by a holographic tweezers beam.We also prove that different particle samples,including gold particles and biological cells,can be applied in our system.This method can be used for different-type optical surface-wave tweezers,with significant potential applications in single-particle spectroscopy,particle sorting,nano-assembly,and others. | XI XIE XIANYOU WANG CHANGJUN MIN HAIXIANG MA YUNQI YUAN ZHANGYU ZHOU YUQUAN ZHANG JING BU XIAOCONG YUAN | 2022 | Photonics Research2022，10，1： | 3 |
| 2 | Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy显示文摘We present a new label-free three-dimensional(3D)microscopy technique,termed transport of intensity diffraction tomography with non-interferometric synthetic aperture(TIDT-NSA).Without resorting to interferometric detection,TIDT-NSA retrieves the 3D refractive index(RI)distribution of biological specimens from 3D intensity-only measurements at various illumination angles,allowing incoherent-diffraction-limited quantitative 3D phase-contrast imaging.The unique combination of z-scanning the sample with illumination angle diversity in TIDT-NSA provides strong defocus phase contrast and better optical sectioning capabilities suitable for high-resolution tomography of thick biological samples.Based on an off-the-shelf bright-field microscope with a programmable light-emitting-diode(LED)illumination source,TIDT-NSA achieves an imaging resolution of 206 nm laterally and 520 nm axially with a high-NA oil immersion objective.We validate the 3D RI tomographic imaging performance on various unlabeled fixed and live samples,including human breast cancer cell lines MCF-7,human hepatocyte carcinoma cell lines HepG2,mouse macrophage cell lines RAW 264.7,Caenorhabditis elegans(C.elegans),and live Henrietta Lacks(HeLa)cells.These results establish TIDT-NSA as a new non-interferometric approach to optical diffraction tomography and 3D label-free microscopy,permitting quantitative characterization of cell morphology and time-dependent subcellular changes for widespread biological and medical applications. | Jiaji Li Ning Zhou Jiasong Sun Shun Zhou Zhidong Bai Linpeng Lu Qian Chen Chao Zuo | 2022 | Light(Science & Applications)2022，11，10： | 2 |
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