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| 1 | 壳聚糖/矿化胶原多孔支架构建及体外成骨分化与生物相容性显示文摘背景:壳聚糖材料具有良好的生物相容性、生物可降解性和低免疫原性,但其活性较差;矿化胶原材料具有良好的生物相容性,但机械性能不足,将两者结合可能会表现出更好的机械性能及成骨活性。目的:构建不同配比的壳聚糖/矿化胶原多孔支架,表征其表面特征与生物相容性。方法:采用冷冻干燥法制备单纯的壳聚糖支架与壳聚糖矿化胶原质量比分别为2∶1、1∶1的壳聚糖/矿化胶原多孔支架,通过扫描电镜、能量色散谱仪、X射线衍射仪、傅里叶红外光谱表征3组支架的表面形貌、元素组成及物相结构。将小鼠成骨前体细胞直接接种于3组支架表面,扫描电镜观察细胞黏附情况。将空白对照及3组材料浸提液分别与小鼠成骨前体细胞共培养,通过CCK-8实验、鬼笔环肽染色及碱性磷酸酶活性分析各组材料对小鼠成骨前体细胞增殖与分化的影响。结果与结论:(1)扫描电镜下可见,单纯壳聚糖多孔支架界面存在明显间隙,壳聚糖/矿化胶原多孔支架界面键合紧密,同时随着支架中壳聚糖含量的减少,矿化胶原颗粒在支架中产生的团聚现象更加明显;能量色散谱仪检测结果显示,单纯壳聚糖支架表面的元素主要为碳、氮、氧,加入矿化胶原后支架材料表面的钙、磷元素逐渐增多;X射线衍射图谱和傅里叶红外光谱显示壳聚糖与矿化胶原为物理性结合;(2)扫描电镜下可见细胞在3组支架材料表面正常黏附和铺展,其中黏附于单纯壳聚糖支架表面的细胞数量较少;(3)CCK-8实验显示,与单纯壳聚糖多孔支架浸提液相比,两种配比的壳聚糖/矿化胶原多孔支架浸提液可促进小鼠成骨前体细胞的增殖;细胞骨架染色显示,两种配比的壳聚糖/矿化胶原多孔支架浸提液组细胞密度较高,细胞丝足相互连接,微丝肌动蛋白表达更清晰;(4)碱性磷酸酶活性检测显示,与单纯壳聚糖多孔支架浸提液相比,两种配比的壳聚糖/矿化胶原多孔支架浸提液可促进小鼠成骨前体细胞的成骨分化;(5)结果表明,壳聚糖/矿化胶原多孔支架可有效促进小鼠成骨前体细胞的增殖与分化。 | 孙溪饶 包佳昕 王程越 | 2022 | 中国组织工程研究2022，26，34： | 2 |
| 2 | Biomechanics and mechanobiology of the bone matrix显示文摘The bone matrix plays an indispensable role in the human body,and its unique biomechanical and mechanobiological properties have received much attention.The bone matrix has unique mechanical anisotropy and exhibits both strong toughness and high strength.These mechanical properties are closely associated with human life activities and correspond to the function of bone in the human body.None of the mechanical properties exhibited by the bone matrix is independent of its composition and structure.Studies on the biomechanics of the bone matrix can provide a reference for the preparation of more applicable bone substitute implants,bone biomimetic materials and scaffolds for bone tissue repair in humans,as well as for biomimetic applications in other fields.In providing mechanical support to the human body,bone is constantly exposed to mechanical stimuli.Through the study of the mechanobiology of the bone matrix,the response mechanism of the bone matrix to its surrounding mechanical environment can be elucidated and used for the health maintenance of bone tissue and defect regeneration.This paper summarizes the biomechanical properties of the bone matrix and their biological significance,discusses the compositional and structural basis by which the bone matrix is capable of exhibiting these mechanical properties,and studies the effects of mechanical stimuli,especially fluid shear stress,on the components of the bone matrix,cells and their interactions.The problems that occur with regard to the biomechanics and mechanobiology of the bone matrix and the corresponding challenges that may need to be faced in the future are also described. | Chunyang Ma Tianming Du Xufeng Niu Yubo Fan | 2022 | Bone Research2022，10，4： | 2 |
| 3 | Fractal Design Boosts Extrusion-Based 3D Printing of Bone-Mimicking Radial-Gradient Scaffolds显示文摘Although extrusion-based three-dimensional(EB-3D)printing technique has been widely used in the complex fabrication of bone tissue-engineered scaffolds,a natural bone-like radial-gradient scaffold by this processing method is of huge challenge and still unmet.Inspired by a typical fractal structure of Koch snowflake,for the first time,a fractal-like porous scaffold with a controllable hierarchical gradient in the radial direction is presented via fractal design and then implemented by EB-3D printing.This radial-gradient structure successfully mimics the radially gradual decrease in porosity of natural bone from cancellous bone to cortical bone.First,we create a design-to-fabrication workflow with embedding the graded data on basis of fractal design into digital processing to instruct the extrusion process of fractal-like scaffolds.Further,by a combination of suitable extruded inks,a series of bone-mimicking scaffolds with a 3-iteration fractal-like structure are fabricated to demonstrate their superiority,including radial porosity,mechanical property,and permeability.This study showcases a robust strategy to overcome the limitations of conventional EB-3D printers for the design and fabrication of functionally graded scaffolds,showing great potential in bone tissue engineering. | Huawei Qu Zhenyu Han Zhigang Chen Lan Tang Chongjian Gao Kaizheng Liu Haobo Pan Hongya Fu Changshun Ruan | 2021 | Research2021，，1： | 2 |
| 4 | Cell-matrix reciprocity in 3D culture models with nonlinear elasticity显示文摘Three-dimensional(3D)matrix models using hydrogels are powerful tools to understand and predict cell behavior.The interactions between the cell and its matrix,however is highly complex:the matrix has a profound effect on basic cell functions but simultaneously,cells are able to actively manipulate the matrix properties.This(mechano)reciprocity between cells and the extracellular matrix(ECM)is central in regulating tissue functions and it is fundamentally important to broadly consider the biomechanical properties of the in vivo ECM when designing in vitro matrix models.This manuscript discusses two commonly used biopolymer networks,i.e.collagen and fibrin gels,and one synthetic polymer network,polyisocyanide gel(PIC),which all possess the characteristic nonlinear mechanics in the biological stress regime.We start from the structure of the materials,then address the uses,advantages,and limitations of each material,to provide a guideline for tissue engineers and biophysicists in utilizing current materials and also designing new materials for 3D cell culture purposes. | Kaizheng Liu Maury Wiendels Hongbo Yuan Changshun Ruan Paul H.J.Kouwer | 2022 | Bioactive Materials2022，7，3： | 0 |
| 5 | 载抗菌肽壳聚糖/骨粉/纳米微晶纤维素颌骨修复支架的理化性能显示文摘背景:单一生物材料较难达到理想骨组织工程支架材料的标准,多种生物材料复合及优势互补是构建出理想支架的有效途径。目的:构建出新型颌骨修复支架,并检测其关键理化性能。方法:采用冷冻干燥法制备羧甲基壳聚糖/骨粉/纳米微晶纤维素/抗菌肽颌骨修复支架,观察分析支架的微观结构,测定支架的抗拉强度、断裂伸长率,评估支架的吸水率、体外降解率、热稳定性和抗菌肽缓释作用。结果与结论:①扫描电镜下可见,制备的颌骨修复支架具有均匀多孔网状结构,平均孔径为114.9μm,支架的吸水率为(698.8±53.8)%,支架的断裂伸长率介于8%-10%,抗拉强度介于0.7-0.9 MPa,应力-应变关系与自然的软骨组织相类似;②热重曲线显示,在200℃以下,颌骨修复支架材料轻微失重;在200-400℃之间,支架材料明显失重;在600℃以上时,支架材料失重严重;③体外药物释放结果显示,该支架在最初的12 h内释放出(58.40±1.79)%的抗菌肽,12 h后药物释放率下降,近20%的抗菌肽在6 d后仍未释放,有利于药效持续稳定;④将支架分别浸泡于PBS与含溶菌酶的PBS中,在28 d时,无溶菌酶与含溶菌酶条件下的支架降解率分别为70%,85%;⑤结果显示,载抗菌肽的羧甲基壳聚糖/骨粉/纳米微晶纤维素支架具有良好的吸水性能、热稳定性、降解性能与机械性能。 | 于爱敏 徐婷 朱韵莹 梁坚强 武东辉 | 2023 | 中国组织工程研究2023，27，25： | 0 |
| 6 | Fractal Design Boosts Extrusion-Based 3D Printing of Bone-Mimicking Radial-Gradient Scaffolds显示文摘Although extrusion-based three-dimensional(EB-3D)printing technique has been widely used in the complex fabrication of bone tissue-engineered scaffolds,a natural bone-like radial-gradient scaffold by this processing method is of huge challenge and still unmet.Inspired by a typical fractal structure of Koch snowflake,for the first time,a fractal-like porous scaffold with a controllable hierarchical gradient in the radial direction is presented via fractal design and then implemented by EB-3D printing.This radial-gradient structure successfully mimics the radially gradual decrease in porosity of natural bone from cancellous bone to cortical bone.First,we create a design-to-fabrication workflow with embedding the graded data on basis of fractal design into digital processing to instruct the extrusion process of fractal-like scaffolds.Further,by a combination of suitable extruded inks,a series of bone-mimicking scaffolds with a 3-iteration fractal-like structure are fabricated to demonstrate their superiority,including radial porosity,mechanical property,and permeability.This study showcases a robust strategy to overcome the limitations of conventional EB-3D printers for the design and fabrication of functionally graded scaffolds,showing great potential in bone tissue engineering. | Huawei Qu Zhenyu Han Zhigang Chen Lan Tang Chongjian Gao Kaizheng Liu Haobo Pan Hongya Fu Changshun Ruan | 2022 | Research2022，，1： | 0 |
| 7 | Advances in biomineralization-inspired materials for hard tissue repair显示文摘Biomineralization is the process by which organisms form mineralized tissues with hierarchical structures and excellent properties,including the bones and teeth in vertebrates.The underlying mechanisms and pathways of biomineralization provide inspiration for designing and constructing materials to repair hard tissues.In particular,the formation processes of minerals can be partly replicated by utilizing bioinspired artificial materials to mimic the functions of biomolecules or stabilize intermediate mineral phases involved in biomineralization.Here,we review recent advances in biomineralization-inspired materials developed for hard tissue repair.Biomineralization-inspired materials are categorized into different types based on their specific applications,which include bone repair,dentin remineralization,and enamel remineralization.Finally,the advantages and limitations of these materials are summarized,and several perspectives on future directions are discussed. | Shuxian Tang Zhiyun Dong Xiang Ke Jun Luo Jianshu Li | 2021 | International Journal of Oral Science2021，13，4： | 0 |
| 8 | 矿化胶原材料的制备、调控及矿化机制研究进展显示文摘骨修复材料是当前临床应用需求量最大的生物医用材料之一.Ⅰ型胶原作为人体的主要结构蛋白,是骨组织的主要成分.胶原纤维所具有的独特四分之一错列结构能够引导纳米粒子沉积而实现矿化,因此常作为模板制备矿化胶原材料以模拟骨骼的多级微纳米结构,应用于骨修复领域.全文综述了矿化胶原材料的种类及制备,重点阐述了胶原纤维的矿化机制及调控因素,并简要展望了矿化胶原材料的发展前景及面临的挑战,为矿化胶原材料的制备及矿化机制的探索提供参考. | 田振华 赵文杰 高盼盼 何静瑄 | 2023 | 陕西科技大学学报2023，41，6： | 0 |
| 9 | 力学环境调控骨基质仿生矿化显示文摘骨缺损一直以来都是威胁人类生命健康的重要原因,人工仿生骨修复替代材料是目前治疗骨损伤最为有效、可行的解决途径之一。要研发人工骨仿生材料,必先构建体外仿生矿化体系,以研究天然骨基质的矿化机制。胶原是矿化发生的模板,其交联度、直径、渗透压和表面电荷等性质会直接影响矿化的进行。矿化发生的生化和力学环境对矿化过程的影响也十分明显,特别是非胶原蛋白和流体切应力。流体切应力是骨组织在微观环境下受到的最主要力学刺激方式,对骨骼生长、修复以及健康维护都具有重要意义。不同水平和加载方式的切应力对无定形磷酸钙向骨磷灰石的转化、胶原纤维的自组装和定向排列以及分层纤维内矿化的形成具有显著作用。本文总结影响骨基质矿化的因素及其作用机制,重点介绍流体切应力对胶原矿化的调控作用,并展望未来的发展方向。 | 马春阳 杜田明 牛旭锋 樊瑜波 | 2022 | 医用生物力学2022，37，2： | 0 |
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