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| 1 | 中国被毛孢的化学成分和生物活性研究进展显示文摘中国被毛孢是冬虫夏草菌真正的无性型,其发酵产品已经被广泛用作保健品和药品的原料。本综述归纳总结了中国被毛孢的化学成分及生物活性研究进展,并对中国被毛孢的未来研究方向进行了展望。 | 杨孟孟 戎林 张行方 赵苗 毕宏涛 魏立新 | 2023 | 青海科技2023，30，2： | 0 |
| 2 | Midkine ameliorates LPS-induced apoptosis of airway smooth muscle cells via the Notch2 pathway显示文摘Objective:To evaluate the effect of midkine on lipopolysaccharide(LPS)-induced airway smooth muscle cells(ASMCs).Methods:LPS-stimulated acute lung injury model was used to analyze the effect of midkine on ASMCs in vitro.Recombinant midkine and midkine siRNA were used to investigate the role of Notch2 signaling pathway.Cell proliferation was assessed using Cell Counting Kit-8 assay.Additionally,apoptosis was measured by flow cytometry and protein and mRNA expression of midkine and Notch2 was assessed by Western blotting and qPCR,respectively.Immunofluorescence analysis was also conducted.Results:LPS increased the mRNA and protein expression of midkine and Notch2.Midkine silencing reduced LPS-induced midkine and Notch2 expression.In addition,midkine silencing further reduced the viability and increased apoptosis of ASMCs induced by LPS,which was attenuated by recombinant midkine.Conclusions:The midkine/Notch2 signaling pathway plays a regulatory role in ASMC proliferation and apoptosis in airway inflammation. | Qi-Feng Huang Bo Wang Yu-Qing Weng Tang Deng Li-Hua Li Jin Qian Qi Li Kai-Wen Lin Dong-Mei Sun Shuang-Qin Xu Hang-Fei Wang Xin-Xin Wu Yuan-Tian Sun Xiao-Ran Liu | 2022 | Asian Pacific Journal of Tropical Biomedicine2022，12，12： | 0 |
| 3 | 基于NF-κB/Twist 1通路二甲双胍对GDM大鼠子代胎鼠Ⅱ型肺泡上皮细胞EMT的作用机制显示文摘目的探讨二甲双胍(Met)对妊娠糖尿病(GDM)大鼠子代胎鼠Ⅱ型肺泡上皮细胞上皮间质转化(EMT)的作用及其机制。方法将SD孕鼠随机分为正常组(Normal组)和GDM组。GDM组孕鼠采用高脂饮食联合小剂量链脲佐菌素(STZ)建立GDM模型,测定空腹血糖水平(FBG)。无菌条件下取胎鼠肺组织,分离肺泡上皮细胞,不同浓度Met处理细胞48 h,MTT法检测细胞存活率。将肺泡上皮细胞随机分为对照组(Control组)、GDM组和Met组,Met组细胞用80μmol/L Met处理48 h,Control组和GDM组细胞不做处理。Transwell实验检测细胞侵袭能力,qRT-PCR检测细胞中上皮钙粘蛋白(E-cad)、神经钙粘蛋白(N-cad)和波形蛋白(Vimentin)mRNA相对表达量,蛋白印迹法检测磷酸化核因子-κB(NF-κB)和Twist1蛋白相对表达量。结果GDM组孕鼠的FBG水平明显高于Normal组(P<0.05);细胞存活率随Met浓度的增加而降低,且具有剂量依赖性(P<0.05);与Control组比较,GDM组侵袭细胞数、N-cad、Vimentin mRNA相对表达量以及p-NF-κB p65和Twist1蛋白相对表达量升高,E-cad mRNA相对表达量降低(P<0.05);与GDM组比较,Met组侵袭细胞数、N-cad、Vimentin mRNA相对表达量及p-NF-κB p65和Twist1蛋白相对表达量降低,E-cad mRNA相对表达量升高(P<0.05)。结论Met可抑制GDM大鼠子代胎鼠Ⅱ型肺泡上皮细胞EMT过程,其可能是通过抑制NF-κB/Twist1信号通路发挥作用。 | 段艳芳 刘风伟 秦业强 赵金梅 何文慧 姚晓玲 | 2022 | 西部医学2022，34，10： | 0 |
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