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| 1 | Perfluorinated iodine alkanes promote the differentiation of mouse embryonic stem cells by regulating estrogen receptor signaling显示文摘Investigating the development toxicity of perfuorinated iodine alkanes(PFIs)is critical,given their estrogenic effects through binding with estrogen receptors(ERs).In the present study,two PFIs,including dodecafuoro-1,6-diiodohexane(PFHx DI)and tridecafuorohexyl iodide(PFHx I),with binding preference to ERαand ERβ,respectively,were selected to evaluate their effects on proliferation and differentiation of the mouse embryonic stem cells(m ESCs).The results revealed that,similar to E_(2),50μmol/L PFHx DI accelerated the cell proliferation of the m ESCs.The PFI stimulation at the exposure concentrations of 2–50μmol/L promoted the differentiation of the m ESCs as characterized by the upregulation of differentiation-related biomarkers(i.e.,Otx2 and Dnmt3β)and downregulation of pluripotency genes(i.e.,Oct4,Nanog,Sox2,Prdm14 and Rex1).Comparatively,PFHx DI exhibited higher induction effect on the differentiation of the m ESCs than did PFHx I.The tests on ER signaling indicated that both PFI compounds induced exposure concentration-dependent expressions of ER signaling-related biomarkers(i.e.,ERα,ERβand Caveolin-1)in the m ESCs,and the downstream ER responsive genes(i.e.,c-fos,c-myc and c-jun)well responded to PFHx I stimulation.The role of ER in PFI-induced effects on the m ESCs was further validated by the antagonistic experiments using an ER inhibitor(ICI).The findings demonstrated that PFIs triggered ER signaling,and perturbed the differentiation program of the m ESCs,causing the potential health risk during early stage of development. | Zhihua Ren Xiaoxi Yang Tingting Ku Qian S.Liu Jiefeng Liang Qunfang Zhou Francesco Faiola Guibin Jiang | 2024 | Journal of Environmental Sciences2024，，3： | 0 |
| 2 | 基于实验和网络药理学探讨丹知青娥方改善下尿路症状的作用和分子机制显示文摘[目的]明确丹知青娥方(DZQE)是否具有调节膀胱功能改善男性下尿路症状(LUTS)的潜在作用,并预测可能的靶点和通路。[方法]用膀胱灌流法检测DZQE对正常雄性Wistar大鼠排尿参数的影响;分离正常大鼠膀胱逼尿肌肌条,用肌条张力实验检测DZQE对膀胱逼尿肌舒缩的调节作用;在中药系统药理学数据库与分析平台(TCMSP)和中医百科全书(ETCM)筛选DZQE中的活性成分和靶点信息;通过GeneCards和DisGeNET数据库获取LUTS疾病靶点;筛选DZQE与LUTS的共同作用靶点,并归属其蛋白质类型;通过Cytoscape_v3.7.1软件构建中药-活性成分-作用靶点网络;通过富集分析数据库(DAVID)v6.8对相关靶点进行基因本体论(GO)分析和京都基因与基因组百科全书通路富集分析(KEGG)信号通路富集分析。[结果]DZQE能够降低雄鼠膀胱漏尿点压(DLPP)和最大排尿压(MVP);促进大鼠膀胱逼尿肌收缩,同时抑制氯化钾(KCl)诱导的肌条过度收缩,呈现双向调节效应。筛选得到DZQE中102个活性成分,共对应258个药物潜在靶点,LUTS疾病靶点246个,其中共同作用靶点47个,DZQE可能作用于LUTS的活性成分59个;GO富集分析涉及平滑肌收缩、血管收缩、丝裂原活化蛋白激酶(MAPK)级联、雌激素应答等生物过程;KEGG分析后得到包括磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(AKt)、甲状腺激素、钙离子、雌激素、肿瘤坏死因子(TNF)、ErbB、MAPK、转化生长因子β(TGF-β)信号通路等88条信号通路。[结论]DZQE可以通过多条信号通路调节膀胱平滑肌舒缩,改善雄鼠下尿路症状。 | 杨晓华 刘二伟 马亚宣 吴垚锌 苗琳 | 2023 | 天津中医药大学学报2023，42，3： | 0 |
| 3 | 槲皮素和木犀草苷促进皮肤伤口愈合机制的实验研究显示文摘目的探究槲皮素、木犀草苷对皮肤伤口再上皮化的影响及机制。方法以不同浓度的槲皮素、木犀草苷单独或联合处理小鼠全层皮肤缺损伤口,隔天观察伤口愈合情况。分别用苏木素_伊红染色和免疫组织化学染色观察伤口边缘皮肤再生情况、细胞增殖及表皮干细胞标志分子、β-catenin、c-Mye、Sox2表达;采用RT-PCR检测Sox2在表皮干细胞的表达。结果槲皮素、木犀草苷对小鼠能促进皮肤伤口愈合,增加伤口周围新生皮肤表皮层厚度,增加表皮基底层中增殖细胞和表皮干细胞数量以及β-catenin.c-Mye和Sox2表达;在体外能促进表皮干细胞表达Sox2。槲皮素和木犀草昔合用对伤口愈合的促进作用具有相加效应。结论槲皮素、木犀草苷通过上调β-catenin、c-Mye、Sox2表达促进表皮干细胞增殖,从而促进伤口愈合,两者合用具有相加效应。 | 左睿 杨元 俞程浩 张苹 张广亮 乐颖影 侯瑞兴 | 2023 | 中华手外科杂志2023，39，1： | 0 |
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