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| 1 | miR-122-5p调节创伤性脑外伤后小胶质细胞极化减弱炎症反应显示文摘目的探讨miR-122-5p对创伤性脑外伤后小胶质细胞凋亡、极化和炎症反应的影响。方法建立创伤性脑外伤(traumatic brain injury,TBI)小鼠模型和细胞模型。使用TBI小鼠脑匀浆刺激星型胶质细胞产生外泌体,microRNA微阵列分析外泌体中显著改变的microRNA。实时荧光定量PCR检测TBI小鼠模型和TBI细胞模型中miR-122-5p表达。使用TUNEL凋亡染色、免疫荧光共聚焦、蛋白质印迹研究miR-122-5p抑制剂在TBI神经炎症中对小胶质细胞凋亡、小胶质细胞M1/M2表型转化、NLRP3通路及NFκB磷酸化的作用。结果通过microRNA微阵列分析发现有83个下调miRNA(改变2倍以上,P<0.05),其中miR-122-5p显著下调(P<0.01),miR-122-5p在TBI小鼠及细胞模型中表达显著下降[(1.0±0.00)vs.(0.41±0.15),P<0.001];[(1.0±0.00)vs.(0.34±0.07),P<0.001]。TUNEL凋亡检测、免疫荧光染色结果表明抑制miR-122-5p表达,可以显著减轻LPS诱导的小胶质细胞凋亡[(8.03±1.30)vs.(3.17±0.34),P<0.001],促进小胶质细胞M1向M2表型转化,即M1表型极化减少[(56.96±13.70)vs.(34.70±3.47),P=0.002],M2表型极化增加[(30.46±3.67)vs.(40.74±2.49),P=0.005]。蛋白质印迹结果表明miR-122-5p抑制剂降低NLRP3炎症小体活化[(0.77±0.10)vs.(0.51±0.11),P=0.02],降低NFκB的磷酸化[(0.73±0.08)vs.(0.50±0.07),P=0.003]。结论miR-122-5p在TBI星形胶质细胞分泌的外泌体及小胶质细胞中表达下调,miR-122-5p抑制剂可以通过抑制TBI后NLRP3炎症小体通路的活化及NFκB的磷酸化,促进小胶质细胞M1向M2表型转化,减少小胶质细胞凋亡,从而减轻TBI后小胶质细胞炎症损伤。 | 康璐璐 龙小兵 王静 吕菁君 | 2022 | 中华急诊医学杂志2022，31，8： | 1 |
| 2 | Efficacy of perampanel as an adjunctive therapy in pediatric focal epilepsy显示文摘Epilepsy is the most common neurological disease,affecting approximately 50 million people worldwide[1].Many novel antiepileptic drugs(AEDs)displaying diverse mechanisms of action have been introduced[2].Although newer AEDs are not significantly superior to traditional AEDs,they have different mechanisms and may be effective in cases where other first-generation drugs have failed[3].A combination of traditional and newer AEDs is now being proposed to improve the efficacy and overall outcomes[4].Perampanel(PER)is a selective and non-competitive alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid(AMPA)receptor antagonist that selectively inhibits AMPA receptormediated synaptic excitation[5,6]. | Wei‑Ran Zhang Liu Liu Lu Xu Yi Hua Xiao‑Jun Su Pei‑Fang Jiang Zhe‑Feng Yuan Feng Gao | 2023 | World Journal of Pediatrics2023，19，11： | 0 |
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