| 1 | Accurate and sensitive determination of hydroxychloroquine sulfate used on COVID-19 patients in human urine,serum and saliva samples by GC-MS显示文摘A rapid,accurate,and sensitive analytical method,ultrasonication-assisted spraying based fine droplet formationeliquid phase microextractionegas chromatographyemass spectrometry(UA-SFDF-LPME-GCMS),was proposed for the determination of trace amounts of hydroxychloroquine sulfate in human serum,urine,and saliva samples.To determine the best extraction strategy,several liquid and solid phase extraction methods were investigated for their efficiencies in isolation and preconcentration of hydroxychloroquine sulfate from biological matrices.The UA-SFDF-LPME method was determined to be the best extraction method as it was operationally simple and provided accurate results.Variables such as the extraction solvent,spraying number,sodium hydroxide concentration and volume,sample volume,mixing method,and mixing period were optimized for the proposed method using the onevariable-at-a-time approach.In addition,Tukey’s method based on a post hoc comparison test was employed to evaluate the significant difference between the parameters inspected.After the optimization studies,the limit of detection(LOD)and limit of quantification(LOQ)were determined to be 0.7 and 2.4 mg/kg,respectively.The sensitivity of the GC-MS system based on the LOD was enhanced approximately 440-fold when the UA-SFDF-LPME method was employed.Spiking experiments were also conducted for the human serum,urine,and saliva samples to determine the applicability and accuracy of the proposed method.Recoveries for the human serum,urine,and saliva samples were found to be in the ranges of 93.9%-101.7%,95.2%-105.0%,and 93.1%-102.3%,respectively.These results were satisfactory and indicated that the hydroxychloroquine sulfate level in the above biological samples could be analyzed using the proposed method. | Süleyman Bodur Sezin Erarpat Omer Tahir Günkara Sezgin Bakırdere | 2021 | Journal of Pharmaceutical Analysis2021，11，3： | 0 |
| 2 | 替代生物样品的绿色样品制备技术在法医毒物学中的研究应用显示文摘法医毒物学是研究死亡案件、药物检测、运动兴奋剂等的重要学科,主要是结合现代仪器分析技术,对体内外未知毒物如安非他命、苯二氮卓类、大麻和阿片类等进行定性、定量分析。替代生物样品如头发、指甲、唾液、汗液、脑脊液等是法医毒物分析主要对象,与常规血液和尿液样品相比,它们具有易于收集、相对稳定和不易变质等优点。在样品制备过程中,为了达到消除基质干扰并进行预富集的目的,目标分析物与分析仪器应相互兼容。测定法医毒物目标分析物的常用仪器有气相色谱仪和液相色谱仪结合质量检测器。目前被广泛应用的是固相萃取技术和液-液萃取技术。此外,随着绿色分析化学的发展,至今已开发了大量的微萃取和微型萃取技术。简要综述了法医毒物学中替代生物样品的绿色样品制备技术的研究应用。 | 赵玉霞 孙青芝 | 2023 | 山东化工2023，52，14： | 0 |
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