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| 1 | 玄参环烯醚萜苷对氧糖剥夺再灌注细胞模型内质网钙稳态的调控作用显示文摘目的:阐明玄参环烯醚萜苷(IGRS)基于调控内质网应激反应对抗脑缺血再灌注损伤的作用及机制。方法:采用IGRS(50、100、200μg/mL)预处理PC12细胞24 h,然后构建氧糖剥夺/再灌注(OGD/R)细胞模型。MTT法检测细胞存活率,细胞内乳酸脱氢酶(LDH)释放法检测细胞损伤程度;流式细胞术检测细胞凋亡率,蛋白质印迹法检测B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、C/EBP同源蛋白(CHOP)、半胱氨酸天冬氨酸蛋白酶12(caspase-12)、葡萄糖调节蛋白78(GRP78)水平;实时逆转录PCR法检测肌浆网钙泵2(SERCA2)、1,4,5-三磷酸肌醇受体1(IP_3R1)、兰尼碱受体2(RyR2)mRNA表达;激光共聚焦显微镜观察细胞质中的游离钙离子浓度。结果:IGRS预处理可以提高OGD/R细胞存活率、减少LDH释放(均P<0.01);降低细胞凋亡率,抑制GRP78、CHOP,Bax和caspase-12蛋白表达(均P<0.01),上调Bcl-2和Bcl-2/Bax(均P<0.01),增加细胞SERCA2 mRNA表达(P<0.01),降低游离钙离子浓度,下调RyR2和IP_3R1 mRNA表达。结论:IGRS具有明确的神经保护作用,可能是通过调节SERCA2维持钙平衡,进而抑制内质网应激介导的细胞凋亡来减轻脑缺血再灌注损伤。 | 叶嘉仪 龚恒佩 王凌峰 黄真 仇凤梅 钟晓明 | 2020 | 浙江大学学报(医学版)2020，49，6： | 3 |
| 2 | Hypothermia selectively protects the anterior forebrain mesocircuit during global cerebral ischemia显示文摘Hypothermia is an important protective strategy against global cerebral ischemia following cardiac arrest.However,the mechanisms of hypothermia underlying the changes in different regions and connections of the brain have not been fully elucidated.This study aims to identify the metabolic nodes and connection integrity of specific brain regions in rats with global cerebral ischemia that are most affected by hypothermia treatment.18F-fluorodeoxyglucose positron emission tomography was used to quantitatively determine glucose metabolism in different brain regions in a rat model of global cerebral ischemia established at 31–33℃.Diffusion tensor imaging was also used to reconstruct and explore the brain connections involved.The results showed that,compared with the model rats established at 37–37.5℃,the rat models of global cerebral ischemia established at 31–33℃had smaller hypometabolic regions in the thalamus and primary sensory areas and sustained no obvious thalamic injury.Hypothermia selectively preserved the integrity of the anterior forebrain mesocircuit,exhibiting protective effects on the brain during the global cerebral ischemia.The study was approved by the Institutional Animal Care and Use Committee at Capital Medical University(approval No.XW-AD318-97-019)on December 15,2019. | Xiao-Hua Wang Wei Jiang Si-Yuan Zhang Bin-Bin Nie Yi Zheng Feng Yan Jian-Feng Lei Tian-Long Wang | 2022 | Neural Regeneration Research2022，17，7： | 0 |
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