| 1 | Significance of changes of gastrointestinal peptides in blood and ileum of experimental spleen deficiency rats显示文摘AIM: To explore the mechanism of spleen deficiency (SD)by studying the relationship of gastro-intestinal peptides level and ileal electro-mechanical activity of SD rats and cold restrain rats.METHODS: (1) spleen deficiency (SD) model was established by feeding Houpou:Zhishi: Dahuang in the ratio of 3:3:2,3 ml/time, for 42 days. (2) The cold restrain stress model:Animals were restrained on grille and placed in a cool water at 18 °C for 3 h. (3) Substance P (SP) and vasoactive intestinal peptide (VIP) levels in all layers of initial part of ileum and blood in rats were measured by radioimmunoassays (RIA)while changes of electric activity and motility in ileum of rats were recorded with electrode and strain gauge.RESULTS: SP levels in ileum and blood of experimental SD rats were significantly higher than that of the control groups (9.89±5.65 vs 1.22±1.18, P<0.005, in ileum; 22.7±3.95 vs6.60±1.47, P<0.001, in blood) while the VIP levels of theSD rats were significantly lower than that of the controls (3.50±2.01 vs9.10±4.91, P<0.05, in ileum; 229.8±62.4 vs560.4±151.3, P<0.001, in blood). As compared with the controls, the average frequency of slow electric waves (21.3±0.96 vs18.2±2.28, P<0.05) and motility (21.5±0.58vs 18±2.65, P<0.005) of SD rats increased obviously and the frequency of fast waves of SD rat also increased. In spontaneous recovery cases, SP levels recovered significantly (compared with the SD groups, 2.99±0.62 vs 9.89±5.65,P<0.001, in ileum; 14.4±4.22 vs 22.7±3.95, P<0.001, in blood) but did not drop to normal. After the SD rats treated with Chinese herbs (Jiawei Sijun zi Tang), SP improved (compared with SD cases, 2.20±1.25 vs9.89±5.65, (P<0.001),in ileum; 10.7±1.88 vs 22.7±3.95, (P<0.001), in blood)and VIP in blood also improved (compared with SD rats,485.7±229.0 vs 229.8±62.4, P<0.01) while the amplitude of motility decreased apparently (compared with the SD rats,0.64±0.096 vs0.89±0.15, P<0.01). The ileal SP levels of cool stress didn't change while the ileal VIP levels of cool stress became significantly lower than that of the control groups (2.87±0.87 vs 9.10±4.91, P<0.01). The blood SP levels of cool stress were significantly higher (15.60±1.83vs 6.60±1.47, P<0.001) whereas the blood VIP levels of cool stress were significantly lower than that of the control group (153.4±70.46 vs 560.4±151.30, P<0.001).CONCLUSION: Changes of SP and VIP levels in initial part of ileum and blood of SD rats and cool stress rats may be closely related to the gastrointestinal motility disorders presented in SD and cool stress rats. the Chinese herbs (Jiawei Sijun zi Tang) currently used have partially therapeutic effect. | Li-Sheng Li Rui-Yao Qu Wei Wang Hua Guo Department of Physiology,Capital University of Medical Sciences,100054,Beijing,China | 2003 | World Journal of Gastroenterology2003，9，3： | 17 |
| 2 | Effects of palmatine on potassium and calcium currents in isolated rat hepatocytes显示文摘AIM: To study the effects of palmatine, a known inhibitoron delayed rectifier potassium current and L-type calciumcurrent (ICa,L) in guinea pig ventricular myocytes, on thepotassium and calcium currents in isolated rat hepatocytes.METHODS: Tight-seal wh ole-cell patch-clamp techniqueswere performed to investigate the effects of palmatine onthe delayed outward potassium currents (IK), inward rectifierpotassium current (IK1) and Ca2+ release-activated Ca2+current (ICRAC) in enzymatically isolated rat hepatocytes.RESULTS: Palmatine 0.3-100 μM reduced IK in a concentationdependent manner with EC50of 41.62±10.11 μM and nH,0.48±0.07 (n=8). The effect of the drug was poorly reversibleafter washout. When the bath solution was changed totetraethylammonium (TEA) 8 mM, IK was inhibited.Palmatine 10 μM and 100 μM shifted the I-V curves of IKdownward, and the block of IK was voltage-independent.Palmatine 0.3-100 μM also inhibited ICRAC in a concentration-dependent manner. The fitting parameters were as follows:ECs0=51.19±15.18 μM, and nH=0.46+0.07 (n=8). The peakvalue of ICRAC in the I-V relationship was decreased bypalmatine 10 μM and 100 μM. But the reverse potential ofIcRAcoCcurred at Voltage=0 mV in all cells. Palmatine 0.3-100 μM failed to have any significant effect on either inwardor outward components of IK1 at any membrane potentialexamined.CONCLUSION: The inhibitory effects on IK and ICRAC couldbe one of the mechanisms that palmatine exerts protectiveeffect on hepatocytes. | FangWang Hong-YiZhou LanCheng GangZhao JiZhou Li-YingFu Wei-XingYao | 2003 | World Journal of Gastroenterology2003，9，2： | 2 |
| 3 | 痰液、血液和纤维支气管镜活检组织中P16^(INK4a)基因甲基化与临床肺癌诊断的关系显示文摘目的探讨血液、痰液和纤维支气管镜活检组织中的P16INK4a基因甲基化与临床肺癌诊断的关系。方法运用甲基化特异度聚合酶链技术(methylation-specific PCR,MSP)检测40例临床诊断肺癌患者和45例一般呼吸道疾病患者血液、纤维支气管镜活检组织和痰液标本中P16INK4a的甲基化状况。比较单一组织来源标本检测和多种组织来源标本联合检测诊断肺癌的诊断特性。结果肺癌患者单一血液、痰液和纤维支气管镜活检组织来源标本中甲基化阳性率分别为54.54%(18/33)、65.00%(13/20)和71.43%(10/14);一般呼吸道疾病患者的单一血液、痰液和纤维支气管镜活检组织来源标本中的甲基化阳性率分别为18.18%(6/33)、23.08%(3/13)和23.53%(4/17);两者比较差异显著(P<0.05);肺癌患者多种组织来源标本联合检测甲基化阳性率为85.00%,和前二者比较差异有显著性(P<0.05)。联合检测诊断试验的准确率为84.71%,阳性预测值82.93%,阴性预测值86.36%,阳性似然比为5.46,阴性似然比0.18。结论肺癌患者痰液、血液和纤维支气管镜活检组织中P16INK4a基因甲基化有助于肺癌诊断,对同一患者获取多种组织来源标本联合检测,可提高检测阳性率。 | 潘江 范惠君 姜燕 马丹 王劲 夏聪 | 2013 | 临床肺科杂志2013，18，6： | 1 |
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