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| 1 | Effects of 60 Hz electromagnetic field exposure on testiculargerm cell apoptosis in mice显示文摘Aim:To evaluate the effects of 60 Hz extremely low frequency (ELF) elelctromagnetic field (EMF) exposure on germ cell apoptosis in the testis of mice.Methods:Adult male BALB/c mice (7 weeks of age) were exposed to a 60 Hz EMF of 0.1 mT or 0.5 mT for 24 h/day.A sham-exposed group served as the control.After 8 weeks of exposure,the mice were sacrificed.Germ cell apoptosis in the testis was assessed by histopathological examination,the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL) and flow cytometric examination of isolated spermatogenic cells stained with 7 aminoactinomycin D (7-AAD).Results:EMF exposure did not significantly affect the body and testis weights,but significantly increased the incidence of germ cell death.The distinguishing morphological feature of EMF exposure was a decrement in the number of well organized seminiferous tubules.Quantitative analysis of TUNEL-positive germ cells showed a significantly higher apoptotic rate in the 0.5 mT exposed mice than that in the sham controls (P<0.05),while the difference between the two exposed groups was insignificant.The TUNEL-positive cells were mainly spermatogonia.In flow cytometryanalysis,the percentage of live cells [forward scatter count (FSC)^hgh7-AAD-] was lower in the exposed groups than that in the controls (Figure 5A),but the decrease in viability was not statistically significant.Conclusion:Continuousexposure to ELF EMF may induce testicular germ cell apoptosis in mice.(Asian J Androl 2004 Mar;6:29-34) | Jin Sang Lee Sang Seok Ahn Kyeong Cheon Jung Yoon-Won Kim Sang Kon Lee | 2004 | Asian Journal of Andrology2004，6，1： | 23 |
| 2 | 精子DNA完整性及对辅助生殖技术治疗结局的影响显示文摘精子遗传物质(包括核DNA和线粒体DNA)易受各种因素影响发生损伤,其损伤机制可能涉及染色质组装异常、氧化应激、凋亡等。精子DNA完整性是成功受精和胚胎发育的基本保证,通过检测精子DNA损伤,评估精子质量,选择优质精子用于临床,可提高辅助生殖技术(ART)成功率,减少技术失败引起的经济、社会和情感问题。本综述主要探讨男性因素不育患者精子DNA完整性及其损伤的可能机制,以及对ART治疗结局的影响。 | 李志凌 刘容菊 | 2006 | 生殖与避孕2006，26，7： | 9 |
| 3 | Sperm DNA damage and its clinical relevance in assessingreproductive outcome显示文摘The routine examination of semen, which assesses sperm concentration, percentage motility and morphology,does not identify subtle defects in sperm chromatin architecture. The focus on the genomic integrity of the male gamete has intensified recently due to the growing concern that genetic diseases may be transmitted via assisted reproductive techniques (ART). Accordingly, the intent of this review is to describe the details of the informationpertaining to mitochondfial/nuclear sperm DNA damage with an emphasis on its clinical significance and its relationship with male infertility. Assessment of sperm DNA damage appears to be a potential tool for evaluating semen samples prior to their use in ART. Testing DNA integrity may help select spermatozoa with intact DNA or with the least amount of DNA damage for use in assisted conception. In turn, this may alleviate the financial, social and emotional problems associated with failed ART attempts. | R. K. Sharma T. Said A. Agarwal | 2004 | Asian Journal of Andrology2004，6，2： | 7 |
| 4 | Effect of prolonged cryptorchidism on germ cell apoptosisand testicular sperm count显示文摘Aim:To evaluate the long term effect of experimental cryptorchidism on germ cell apoptotic rate and testicular sperm content in adult rats.Methods:Bilateral cryptorchidism was created in 40 adult male Sprague-Dawley rats by surgically manipulating the testes into the abdominal cavity and closing the internal inguinal ring.The rats were sacrificed and the testes removed 6 hours and 2,4,7,21,28 and 56 days after cryptorchidism.Germ cell apoptosis was quantified by means of TUNEL assay and apoptosis was confirmed using transmission electron microscopy.Results:The rate of apoptosis peaked at 4 days of cryptorchidism and then progressively declined to a nadir at 14 days of cryptorchidism.At 56 days of cryptorchidism,the germinal epithelium was largely depleted by the apoptotic process and only a few mature sperm were seen within the testis.At this point,a few tubules were seen to be repopulating with primary spermatocytes and the level of germ cell apoptosis began to increase marginally.Testicular sperm count (TSC) began to decline rapidly at day 7 of cryptorchidism.Only a few mature sperm were found in the testes of rats following 56 days of cryptorchidism.Multinucleated giant cells (MGC) were most numerous within the seminiferous tubules at day 4.At day 7,35% of MGCs were TUNEL positive.At all subsequent time points,however,MGCs fail to stain positive for apoptosis.This resumption of increased apoptosis coincided with the appearance of a population of primary spermatocytes in some seminiferous tubules.Moreover,there was not a corresponding increase in the number of mature sperm after 56 days of cryptorchidism.Conclusion:The decline in germ cell apoptosis after 4 days of cryptorchidism can be attributed to be the result of an overall depletion of germ cells.It appears that after a prolonged cryptorchidism (56 days),there is a limited resumption of spermatogenesis presumably as a result of a decrease in the maturing germ cells undergoing programmed cell death.(Asian JAndrol2004 Mar;6:47-51) | Albaha Barqawi Herald Trummer Randall Meacham | 2004 | Asian Journal of Andrology2004，6，1： | 7 |
| 5 | 男性不育症诊治新进展显示文摘辅助生殖技术,特别是卵泡浆内精子注射技术应用于治疗男性不育,对难治性男性不育治疗取得突破性进展,但对于每一男性不育个体选择治疗时,仍应关注传统诊疗方法。对男性生殖相关的遗传学研究和干细胞工程研究受到重视。 | 王益鑫 | 2010 | 现代泌尿外科杂志2010，15，2： | 5 |
| 6 | 生活方式及职业与男性不育显示文摘 | 刘忠平 李质馨 田洪艳 徐冶 潘晓燕 | 2010 | 医药论坛杂志2010，31，9： | 3 |
| 7 | 热应激对生精细胞影响机制的研究进展显示文摘精子的形成是一个极其复杂的过程,热应激引起生精障碍的因素又是多种多样,本文拟综述热应激诱导生精细胞凋亡、生精细胞DNA完整性的破坏、HSP在热应激诱导生精障碍中的作用以及热应激诱导生精细胞微环境变化,并展望今后的研究方向。 | 田洪成 马志芳 杨波 陈予军 马良宏 马文智 | 2017 | 临床泌尿外科杂志2017，32，2： | 3 |
| 8 | Uchl1及其关联蛋白参与小鼠实验性隐睾中精母细胞凋亡(英文)显示文摘以往研究显示Uchl1参与调节生理状况下小鼠精母细胞的凋亡。本文以小鼠实验性单侧隐睾为研究模型,以切除单侧睾丸和假手术作为对照,用苏木精-伊红(HE)染色和DNA末端标记(TUNEL)观察生精细胞的形态和凋亡情况;用免疫组化分析Uchl1及其相关蛋白Jab1和p27kip1在隐睾症的热应激反应导致精母细胞损失过程中的变化情况,并用亲和分析(pull-down)和免疫荧光共定位检测三种蛋白在精母细胞的关联性。结果显示,Jab1和p27kip1,与Uch1平行,在具凋亡形态的精母细胞中响应热应激而含量增加,而在多核巨细胞中无类似变化。Jab1可以与睾丸蛋白提取物中Uchl1结合,并与Uchl1和p27kip1特异性地在具凋亡形态的精母细胞中共定位。以上结果提示,Uchl1蛋白的积累参与隐睾中热应激诱导的生精细胞凋亡过程,但不影响接下来的多核巨细胞的形成,且Uchl1蛋白的作用机制涉及Jab1和p27kip1参与的一种新途径。 | 杜平 姚余卫 石燕 顾正 王健 孙兆贵 左嘉客 | 2014 | 生理学报2014，66，5： | 3 |
| 9 | 卵丘细胞凋亡对体外培养卵母细胞发育潜能的影响显示文摘目的研究体外培养中卵丘细胞凋亡对卵母细胞结构的影响,探讨体外受精中可用卵丘细胞凋亡率预测卵母细胞发育潜能的原因。方法对GV期人卵丘-卵母细胞复合体进行体外成熟培养,用HE染色、DAPI染色和原位末端标记(TUNEL)法标记3种方法对单卵卵丘细胞凋亡率进行检测。分为凋亡率高和低的2组,用光镜和透射电镜观察卵母细胞的结构。对MⅡ期卵母细胞进行体外受精,培养2d后在光镜下评价胚胎质量。结果卵丘细胞凋亡率低的卵母细胞结构和发育潜能良好;卵丘细胞凋亡率高的卵母细胞发育潜能差,存在各种结构异常,包括围卵周隙不均,对应不同部位的细胞质发育不同步;细胞质中出现堆积不均的细胞器团、次级溶酶体、及可能由溶酶体降解造成的大量空隙;线粒体外膜和嵴模糊、膨大,呈现凋亡迹象;第一极体碎裂;透明带异常增厚或变薄。相应的卵丘细胞微绒毛和细胞连接减少。结论揭示了卵丘细胞凋亡对卵母细胞结构的影响,揭示了卵丘细胞凋亡率影响卵母细胞发育潜能的原因。 | 杨永杰 张燕君 李媛 | 2007 | 解剖学报2007，38，6： | 1 |
| 10 | 不同温度和持续时间对体外精子活力的影响显示文摘目的:探讨不同温度和持续时间对体外精子活力的影响。方法:采用自身前后对照法,在知情同意原则下,将30份正常育龄期男性精液标本分别置于17℃、26℃、30℃、37℃4个不同温度点下,于10min、25 min、40 min、60min 4个不同时间点,使用计算机辅助精子分析(CASA)技术观察前向运动精子浓度的前后变化情况,从而分析温度、时间与精子活力之间的关系。结果:在同一时间点,30份精液标本的平均前向运动精子浓度与温度呈线性相关,当温度在26℃时前向运动精子浓度最高(P<0.05);在同一温度下,平均前向运动精子浓度与时间同样呈线性相关,当时间处于40min时,平均前向运动精子浓度最高(P<0.05)。结论:准确把握精子在不同温度和不同时间下的活力,能够提高精液检查中对精子质量判断的准确性,对指导精液处理技术有一定的参考价值。 | 李刚 杨海超 钟继生 高郁森 | 2018 | 中国性科学2018，27，7： | 1 |
| 11 | A rare diagnosis:testicular dysgenesis with carcinoma in situ detected in a patient with ultrasonic microlithiasis显示文摘一个稀罕盒子被介绍在哪儿有微侵略原位癌的一个 dysgenetic 睾丸( CIS ,未分类的类型的另外已知的同样小管内的细菌房间瘤[ IGCNU ]并且阴囊的 intraepithelial 瘤形成[锡])在温和阴囊的疼痛和超声的阴囊的微结石地介绍的一个32岁的人与到睾丸网和间质组织的微侵略被发现。超声和 CIS 的协会的知识是重要的在一个公开细菌房间肿瘤的开发以前在阶段诊断病人。病人把四混乱中的三个认为阴囊的发育不全症候群(TDS ) 的症状:dysgenetic 与 CIS 离开了睾丸,温和左边的隐睾病(高放的阴囊的低亚硫酸钠营养的睾丸) 并且稍微减少的精液质量。因此,这应该被记住有一 TDS 症状的一个病人可以港口其它,甚至 CIS 或阴囊的癌症。因此,有一 TDS 症状的病人应该为其它的存在被检查,并且如果一个人是在场的更多,额外的担心被保证。 | Christina E.Hoei-Hansen Peter Sommer Ewa Rajpert-De Meyts Niels E.Skakkebaek | 2005 | Asian Journal of Andrology2005，7，4： | 1 |
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