共被期刊论文引用了4次
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| 1 | 横纹肌相关含硒蛋白SelW的研究进展显示文摘 | 王晓龙 | 2004 | 中国兽医科技2004，34，11： | 4 |
| 2 | SelW基因转录后沉默对小鼠骨骼肌细胞内GSH及GPx影响研究显示文摘通过降低骨骼肌细胞内硒蛋白W的表达,考察其对GSH和GPx的影响,针对个别研究结果与生物信息学研究结果矛盾的焦点深入研究。利用RNAi敲除小鼠骨骼肌细胞中SelW基因,经定量PCR和Western blot-ting鉴定后,进行WST、GSH含量和GPx活力检测。结果表明,RNAi后目的基因mRNA和蛋白表达量降低了71.9%和68.8%。WST检测结果显示阳性组比对照组下降了21.5%;GSH、GPx检测结果显示阳性组比对照组分别增加了29.76%和47.58%。结果显示,SelW敲除引发细胞的毒性作用,并导致GSH和GPx代偿性增高,与生物信息学关于SelW具有抗氧化功能的研究结果相互验证。 | 王晓龙 许凯 秦藕菊 杨传平 | 2009 | 畜牧兽医学报2009，40，7： | 4 |
| 3 | SelW mRNA下调对肌细胞状态影响的FCM评估显示文摘运用RNA干扰技术,在转录后阶段实现肌细胞中SelW mRNA的定量下调,并利用流式细胞术客观地区分、计数不同状态的细胞,考察C2C12细胞中SelW mRNA的定量下调对细胞状态的影响。结果显示:当siR-NA剂量为2.0,1.5,1.2μg/g时,细胞脱落、死亡;当siRNA剂量为1.0,0.5,0.25μg/g时,凋亡率分别为32.5%,12.4%,6.58%。在干预评估试验中,阴性对照组与空白对照组差异显著(P<0.01)。细胞状态随SelW mRNA的下调存在着阶段性变化,凋亡率与mRNA的降低量之间的关系为非线性正相关,证明SelW对肌细胞的健康是必须的。显著的凋亡率表明SelW对肌细胞的功能重要性的机制是十分复杂的,尚需进一步研究。 | 王晓龙 宋晓东 宋月峰 徐伟 | 2007 | 吉林农业大学学报2007，29，2： | 1 |
| 4 | The Influence of SelW on GSH and GPx after Post-Transcriptional Gene Silencing in Mouse Skeletal Muscle Cells显示文摘The expression of Selenoprotein W(SelW)in C2C12 skeletal muscle cells was specifically decreased to examine its influence on the amount of glutathione(GSH)and the activity of glutathione peroxidase(GPx).SelW knock-down was performed by RNA interference(RNAi)in cultured muscle cells and verified by Real-time PCR and Western blotting.In addition,cell viability,GSH content and GPx activity were assayed.The results showed that the mRNA level and protein expression of SelW were decreased successfully by 71.9%and 68.8%relative to control values,cell viability decreased by 21.5%,GSH increased by 29.76%,and GPx increased by 47.58%.WST assay showed that compared with blank control,the value of positive group dropped 21.5%;In GSH and GPx assay,compared with blank control the positive group increased29.76%and 47.58%separately.In conclusion,SelW knock-down by RNAi caused significant cytotoxity in skeletal muscle cells and led to compensatory increases in GSH content and GPx activity.These findings are consistent with the suggestions from bioinformatics indicating an antioxidative role for SelW in skeletal muscle cells. | WANG Xiao-long XU Kai QIN Ou-ju CHEN Xiao-feng YANG Chuan-ping | 2009 | 畜牧兽医学报2009，40，S1： | 0 |
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