| 1 | 基于信息可视化数据挖掘隋唐至明清时期针灸治疗中风后半身不遂选穴规律研究显示文摘目的运用信息可视化数据挖掘技术研究隋唐至明清时期相关文献针灸治疗中风后半身不遂的选穴规律及穴位配伍意义。方法整理隋唐至明清时期相关文献针灸治疗中风后半身不遂的处方,经筛选后利用信息可视化软件Microsoft Access 2017建立数据库,运用信息可视化软件SPSS 22.0、Modeler 18.0对治疗本病穴位、经络等行统计分析。结果本研究获得隋唐至明清时期治疗中风后半身不遂的针灸处方243条,涉及穴位163个,遍布所有正经及任、督二脉,穴位针刺的总频数为1872次,其中针刺经穴频数排前3位的经络是手阳明大肠经、足少阳胆经、足阳明胃经;针刺频数排前10位的经穴为:曲池、足三里、昆仑、太溪、百会、阳陵泉、合谷、悬钟、环跳、委中;聚类分析获得5个有效聚类群:风市-足三里-百会、曲池-昆仑-太溪、昆仑-涌泉-太溪、风池-风府、环跳-委中-阳陵泉-风市-悬钟;关联规则分析支持度排前6位的有风市-足三里、合谷-悬钟、悬钟-曲池、合谷-风市、昆仑-太溪、曲池-阳陵泉;特定穴位的针刺频率高达91.29%,其中五腧穴使用频数最多,其次为两脉交会穴及原穴。结论运用信息可视化数据挖掘技术对隋唐至明清时期治疗中风后半身不遂的针灸处方进行规律挖掘,发现其选穴范围较广,常选用四肢部位经穴,注重阳经经穴及特定穴的应用,选取经穴方法多以局部选穴、辨证选穴及循经取穴为主,可为研究中医药古代典籍、针灸治疗中风后半身不遂等提供新思路。 | 刘伟 艾坤 唐旖雯 唐琴 雷欢 | 2020 | 湖南中医药大学学报2020，40，8： | 8 |
| 2 | Characteristics of neural growth and cryopreservation of the dorsal root ganglion using three-dimensional collagen hydrogel culture versus conventional culture显示文摘In vertebrates, most somatosensory pathways begin with the activation of dorsal root ganglion(DRG) neurons. The development of an appropriate DRG culture method is a prerequisite for establishing in vitro peripheral nerve disease models and for screening therapeutic drugs. In this study, we compared the changes in morphology, molecular biology, and transcriptomics of chicken embryo DRG cultured on tissue culture plates(T-DRG) versus three-dimensional collagen hydrogels(C-DRG). Our results showed that after 7 days of culture, the transcriptomics of T-DRG and C-DRG were quite different. The upregulated genes in C-DRG were mainly related to neurogenesis, axon guidance, and synaptic plasticity, whereas the downregulated genes in C-DRG were mainly related to cell proliferation and cell division. In addition, the genes related to cycles/pathways such as the synaptic vesicle cycle, cyclic adenosine monophosphate signaling pathway, and calcium signaling pathway were activated, while those related to cell-cycle pathways were downregulated. Furthermore, neurogenesis-and myelination-related genes were highly expressed in C-DRG, while epithelial–mesenchymal transition-, apoptosis-, and cell division-related genes were suppressed. Morphological results indicated that the numbers of branches, junctions, and end-point voxels per C-DRG were significantly greater than those per T-DRG. Furthermore, cells were scattered in T-DRG and more concentrated in C-DRG, with a higher ratio of 5-ethynyl-2′-deoxyuridine(EdU)-positive cells in T-DRG compared with C-DRG. C-DRG also had higher S100 calcium-binding protein B(S100 B) and lower α-smooth muscle actin(α-SMA) expression than T-DRG, and contained fewer terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)-positive cells after 48 hours of serum starvation. After cryopreservation, C-DRG maintained more intact morphological characteristics, and had higher viability and less TUNEL-positive cells than T-DRG. Furthermore, newly formed nerve bundles were able to grow along the existing Schwann cells in C-DRG. These results suggest that C-DRG may be a promising in vitro culture model, with better nerve growth and anti-apoptotic ability, quiescent Schwann cells, and higher viability. Results from this study provide a reference for the construction, storage, and transportation of tissue-engineered nerves. The study was approved by the Ethics Committee of Aier School of Ophthalmology, Central South University, China(approval No. 2020-IRB16), on March 15, 2020. | Ze-Kai Cui Shen-Yang Li Kai Liao Zhi-Jie Wang Yong-Long Guo Luo-Sheng Tang Shi-Bo Tang Jacey Hongjie Ma Jian-Su Chen | 2021 | Neural Regeneration Research2021，16，9： | 2 |
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