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| 1 | 抗生素微生物检定法测定酒石酸泰万菌素预混剂的有效含量显示文摘对抗生素微生物检定法测定酒石酸泰万菌素预混剂进行了方法学验证,确定了最适菌悬液加入量和超声时间。结果表明,泰万菌素浓度在2~10 IU/mL范围内线性关系良好;平均回收率为100.24%,RSD为1.21%,准确度良好;精密度RSD为1.15%,精密度良好;辅料对含量测定无影响,专属性良好;不同培养时间和不同厂家的培养基测定结果RSD分别为1.94%和0.20%,方法耐用性良好。该方法适用于酒石酸泰万菌素预混剂的含量检测。 | 田新岳 张雯 马春芳 吴春燕 邵倩 崔生玲 杨奇 余永涛 | 2022 | 动物医学进展2022，43，4： | 3 |
| 2 | Optimized determination of airborne tetracycline resistance genes in laboratory atmosphere显示文摘Antibiotic resistance genes(ARGs)have been detected in various atmospheric environments.Airborne ARGs transmission presents the public health threat.However,it is very difficult to quantify airborne ARGs because of the limited availability of collectable airborne particulate matter and the low biological content of samples.In this study,an optimized protocol for collecting and detecting airborne ARGs was presented.Experimental results showed that recovery efficiency tended to increase initially and then declined over time,and a range of 550-780 copies/mmz of capture loading was recommended to ensure that the recovery efficiency is greater than 75%.As the cell walls were mechanically disrupted and nucleic acids were released,the buffer wash protects ARGs dissolution.Three ratios of buffer volume to membrane area in buffer wash were compared.The highest concentrations of airborne ARGs were detected with 1.4μL/mm^2 buffer wash.Furthermore,the majority of the cells were disrupted by an ultrasonication pretreatment(5 min),allowing the efficiency ARGs detection of airborne samples.While,extending the ultrasonication can disrupt cell structures and gene sequence was broken down into fragments.Therefore,this study could provide a theoretical basis for the efficient filter collection of airborne ARGs in different environments.An optimized sampling method was proposed that the buffer wash was 1.4 nL/mm and the ultrasonication duration was 5 min.The indoor airborne ARGs were examined in accordance with the improved protocol in two laboratories.The result demonstrated that airborne ARGs in an indoor laboratory atmosphere could pose the considerable health risk to inhabitants and we should pay attention to some complicated indoor air environment. | Lu Song Can Wang Yizhu Wang | 2020 | Frontiers of Environmental Science & Engineering2020，14，6： | 1 |
| 3 | Influences and mechanisms of nanofullerene on the horizontal transfer of plasmid-encoded antibiotic resistance genes between E.coli strains显示文摘The spread and development of antibiotic resistance globally have led to severe public health problems.It has been shown that some non-antibiotic substances can also promote the diffusion and spread of antibiotic resistance genes(ARGs).Nanofullerene(nC60)is a type of nanomaterial widely used around the world,and some studies have discovered both the biological toxicity and environmental toxicity of nC6O .In this study,cellular and molecular biology techniques were employed to investigate the influences of nC6O at sub-minimum inhibitory concentrations(sub-MICs)on the conjugation of ARGs between the E.coli strains.Compared with the control group,nC6O significantly increased the conjugation rates of ARGs by 1.32-10.82 folds within the concentration range of 7.03-1800μg/L.This study further explored the mechanism of this phenomenon,finding that sub-MICs of nC6O could induce the production of reactive oxygen species(ROS),trigger SOSresponse and oxidative stress,affect the expression of outer membrane proteins(OMPs)genes,increase membrane permeability,and thus promote the occurrence of conjugation.This research enriches our understanding of the environmental toxicity of nC6O,raises our risk awareness toward nC6O ,and may promote the more rational employment of nC6O materials. | Qingkun Ji Caihong Zhang Dan Li | 2020 | Frontiers of Environmental Science & Engineering2020，14，6： | 1 |
| 4 | The role of emerging organic contaminants in the development of antimicrobial resistance显示文摘Antimicrobial resistance(AMR)threatens human and ecological health worldwide.Unless major changes occur across the human,animal and environmental sectors,the problem will continue to expand.An important component of AMR that deserves greater attention is the influence of emerging organic contaminants(EOCs)e ubiquitous compounds found,amongst others,in pharmaceuticals,personal care products,food,industrial and agricultural products,plastics and building materials.EOCs are widely used and can accumulate in the environment from varied sources,predominantly via waste streams.EOCs can interact with microbial communities potentially leading to the emergence and spread of AMR.Biocides and pharmaceuticals have been demonstrated to promote AMR development.Antimicrobial resistance is a multi-faceted problem that requires input from all sectors,with robust strategies and policies needed to make headway with solving the issues of this important threat. | Izzie Alderton Barry R.Palmer Jack A.Heinemann Isabelle Pattis Louise Weaver Maria J.Gutierrez-Gines Jacqui Horswell Louis A.Tremblay | 2021 | Emerging Contaminants2021，7，1： | 0 |
| 5 | Evaluating heavy metal contamination of riverine sediment cores in different land-use areas显示文摘Anthropogenic activities are regarded as the main sources of heavy metal pollution,yet few studies have investigated the effects of land-use setting on heavy metal accumulation in riverine sediments.Based on both total contents and geochemical fractions,heavy metal pollution,risk and toxicity were determined in riverine sediment cores from different land-use areas(mountain area-MA,farm area-FA,city area-CA,and industrial area-LA)of the Yang River Basin in North China.The results showed that FA had higher contents of riverine sedimentary Cu;CA had higher contents of Cd;LA.had higher contents of both Cd and Zn.Most riverine sediments from FA and IA were contaminated with the investigated metals,although these concentrations were evaluated to have low potential ecological risk and no toxicity to benthic organisms.However,a high proportion of Cd in the B1 fraction of riverine sediments in IA indicating high risk should receive more attention.The B1 fraction largely determined the contamination,risk and toxicity levels associated with heavy metals in the riverine sediments of the Yang River Basin. | Wenzhong Tang Liu Sun Limin Shu Chuang Wang | 2020 | Frontiers of Environmental Science & Engineering2020，14，6： | 0 |
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