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| 1 | 神经生长因子干预大鼠骨骼肌卫星细胞增殖及α-肌动蛋白的表达显示文摘背景:临床研究证实,向神经受损部位注射神经生长因子可改善骨骼肌的运动功能。目的:观察神经生长因子对大鼠原代骨骼肌卫星细胞增殖的影响。方法:采用组织块培养法培养大鼠原代骨骼肌卫星细胞。将第3代骨骼肌卫星细胞分3组培养:对照组加入培养液,低、高浓度组分别加入含10,20 U/mL神经生长因子的培养液。培养2,4,6 d时,倒置相差显微镜与苏木精-伊红染色观察细胞形态,免疫组化染色观察细胞α-肌动蛋白表达;培养1,2,3,4 d后,采用CCK-8法检测细胞增殖活性。结果与结论:①倒置相差显微镜显示,随着培养时间的增加,各组骨骼肌卫星细胞数量逐渐增多,细胞形态由圆形逐渐变为细长的梭形、纺锤形或多角形,并逐渐融合并沿同一个方向排列,并且高浓度组细胞数量多于低浓度组、对照组(P<0.05);②苏木精-伊红染色显示,各组骨骼肌卫星细胞轮廓清晰可见,密集分布,胞核较大且深染,组间无明显差异;③免疫组化染色显示,各组均可见α-肌动蛋白阳性表达,3组间α-肌动蛋白阳性表达吸光度值比较差异无显著性意义(P>0.05);④CCK-8检测显示,低、高浓度组细胞活力高于对照组(P=0.000);⑤结果表明,高、低浓度的神经生长因子均能促进骨骼肌卫星细胞的增殖,但对α-肌动蛋白表达无影响。 | 颜南 司晓凤 曾亮 田伟 单广东 熊礼硕 杨炜杰 王正东 | 2021 | 中国组织工程研究2021，25，13： | 2 |
| 2 | Inflammation induces zebrafish regeneration显示文摘Tissue or organ regeneration is a complex process with successful outcomes depending on the type of tissue and organism. Upon damage, mammals can only efficiently restore a few tissues including the liver, skin, epithelia of the lung, kidney, and gut. In contrast, lower vertebrates such as zebrafish possess an extraordinary regeneration ability, which restores the normal function of a broad spectrum of tissues including heart, fin, brain, spinal cord, and retina. This regeneration process is either mediated by the proliferation of resident stem cells, or cells that dedifferentiate into a stem cell-like. In recent years, evidence has suggested that the innate immune system can modulate stem cell activity to initiate the regenerative response to damage. This review will explore some of the newer concepts of inflammation in zebrafish regeneration in different tissues. Understanding how inflammation regulates regeneration in zebrafish would provide important clues to improve the therapeutic strategies for repairing injured mammalian tissues that do not have an inherent regenerative capacity. | Maria Iribarne | 2021 | Neural Regeneration Research2021，16，9： | 1 |
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