| 1 | 绝经后骨质疏松症差异microRNA表达及靶基因分析显示文摘目的分析绝经后骨质疏松症(PMOP)女性外周血及骨组织中microRNA(miRNA)表达谱的变化,并对差异miRNA进行靶基因分析,为研究PMOP发病机制、筛选早期诊断标志物及骨质疏松性骨折的预防提供依据.方法获取公共数据平台NCBI-GEO DataSets中的PMOPmiRNA研究数据:第一组为GSE64433数据集,包含23例PMOP患者及25例对照者的外周血样本miRNA表达谱;第二组为GSE74209数据集,包含6例PMOP患者及6例对照者的股骨颈骨组织样本miRNA表达谱.运用R/Bioconductor进行数据处理及差异miRNA分析,筛选每组中PMOP与对照者间表达倍数改变>2,P<0.05的miRNA为组间差异表达miRNA.结合TargetScan、miRDB、miRTarBase数据库进行miRNA靶基因预测,选取在三个数据库中均存在的靶基因,运用Cytoscape绘制并分析miRNA-靶基因调控网络图.结果在第一组中(GSE64433数据集),PMOP外周血筛选出224个差异表达miRNA(75个上调miRNA,149个下调miRNA);在第二组中(GSE74209数据集),PMOP股骨颈骨组织中筛选出132个差异表达miRNA(58个上调miRNA,74个下调miRNA).将第一组及第二组中的差异miRNA取交集,得到8个miRNA在PMOP均呈下调改变,共调控327个靶基因,且其中10个靶基因受到两个miRNA的共同调控.结论PMOP miRNA-靶基因调控网络中的核心miRNA及受到多个miRNA共同调控的靶基因可能在其发病与进展中起到了一定的作用,有作为疾病分子标志物的潜在应用价值.这对PMOP的发生发展、诊断标志物筛选及骨质疏松性骨折预防等相关研究有重要意义. | 黄大耿 郝定均 张峰 贺宝荣 孙宏慧 赵勤鹏 刘翠翠 王冀邯 | 2019 | 中华创伤杂志2019，35，11： | 7 |
| 2 | Transcriptomic analysis reveals essential microRNAs after peripheral nerve injury显示文摘Studies have shown that microRNAs(miRNAs) mediate posttranscriptional regulation of target genes and participate in various physiological and pathological processes, including peripheral nerve injury. However, it is hard to select key miRNAs with essential biological functions among a large number of differentially expressed miRNAs. Previously, we collected injured sciatic nerve stumps at multiple time points after nerve crush injury, examined gene changes at different stages(acute, sub-acute, and post-acute), and obtained mRNA expression profiles. Here, we jointly analyzed mRNAs and miRNAs, and investigated upstream miRNAs of differentially expressed mRNAs using Ingenuity Pathway Analysis bioinformatic software. A total of 31, 42, 30, and 23 upstream miRNAs were identified at 1, 4, 7, and 14 days after rat sciatic nerve injury, respectively. Temporal expression patterns and biological involvement of commonly involved upstream miRNAs(miR-21, let-7, miR-223, miR-10 b, miR-132, miR-15 b, miR-127, miR-29 a, miR-29 b, and miR-9) were then determined at multiple time points. Expression levels of miR-21, miR-132, miR-29 a, and miR-29 b were robustly increased after sciatic nerve injury. Biological processes involving these miRNAs include multicellular organismal response to stress, positive regulation of the epidermal growth factor receptor signaling pathway, negative regulation of epithelial cell differentiation, and regulation of myocardial tissue growth. Moreover, we constructed mechanistic networks of let-7, miR-21, and miR-223, the most significantly involved upstream miRNAs. Our findings reveal that multiple upstream miRNAs(i.e., let-7, miR-21, and miR-223) were associated with gene expression changes in rat sciatic nerve stumps after nerve injury, and these miRNAs play an important role in peripheral nerve regeneration. This study was approved by the Experimental Animal Ethics Committee of Jiangsu Province of China(approval No. 20190303-18) on March 3, 2019. | Yu Wang Shu Wang Jiang-Hong He | 2021 | Neural Regeneration Research2021，16，9： | 4 |
您所在的机构暂未开通此部分的功能，请联系管理员