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| 1 | 黏虫MagR和Cry2基因的时空表达分析显示文摘为了明确黏虫MagR、Cry2基因在定向行为中的作用,本研究采用基因序列分析及实时荧光定量技术,研究了黏虫MagR、Cry2基因的基因序列特征及其表达模式。结果表明,黏虫MagR基因编码131个氨基酸,蛋白分子量为14.17 kD,等电点为9.3,具有多个铁硫簇结合位点;黏虫Cry2基因编码757个氨基酸,蛋白分子量为87.04 kD,等电点为8.73。羽化后不同日龄迁飞型黏虫成虫各部位Cry2基因表达量存在显著差异。羽化后3 d成虫头部和胸部Cry2基因表达量显著高于其他日龄。迁飞型黏虫成虫羽化后不同日龄各部位MagR的表达量具有显著性差异;5 d时各部位的表达量达到最高,各日龄头部和胸部的表达量均显著高于腹部。 | 谢殿杰 张蕾 程云霞 江幸福 | 2021 | 植物保护2021，47，3： | 2 |
| 2 | Geomagnetic field absenee reduces adult body weight of a migratory insect by disrupt!ng feeding behavior and appetite regulation显示文摘The geomagnetic field(GMF)is well documented for its essential role as a cue used in animal orientation or navigation.Recent evidence indicates that the absence of GMF(mimicked by the near-zero magnetic field,NZMF)can trigger stress-like responses such as reduced body weight,as we have previously shown in the brown planthopper,Nilaparvata lugens.In this study,we found that consistent with the significantly decreased body weight of newly emerged female(—14.67%)and male(—13.17%)adult N.lugens,the duration of the phloem ingestion feeding waveform was significantly reduced by 32.02%in 5th instar nymphs reared under the NZMF versus GMF.Interestingly,5th instar nymphs that exhibited reduced feeding had significantly higher glucose levels(+16.98%and+20.05%;24 h and 48 h after molting),which are associated with food aversion,and expression patterns of their appetite-related neuropeptide genes(neuropeptide F,dow regulated overall;short neuropeptide F,dowregulated overall;adipokinetic hormone up-regulated overall;and adipokinetic hormone receptor,down-regulated overall)were also altered under the absence of GMF in a manner consistent with diminishing appetite.Moreover,the expressions of the potential magnetosensor croptochromes(Crys)were found significantly altered under the absence of GMF,indicating the likely upstream signaling of the Cry-mediated magnetoreception mechanisms.These findings support the hypothesis that strong changes in GMF intensity can reduce adult body weight through affecting insect feeding behavior and underlying regulatory processes including appetite regulation.Our results highlight that GMF could be necessary for the maintenanee of energy homeostasis in insects. | Gui-Jun Wan Shou-Lin Jiang Ming Zhang Jing-Yu Zhao Ying-Chao Zhang Wei-Dong Pan Gregory A.Sword Fa-Jun Chen | 2021 | Insect Science2021，28，1： | 1 |
| 3 | 铁硫簇组装蛋白在意大利蜜蜂耐受吡虫啉毒害中的应答分析显示文摘【目的】用生物信息学方法鉴定意大利蜜蜂(Apis mellifera ligustica)铁硫簇组装蛋白(AmIscA),分析它在意大利蜜蜂工蜂耐受杀虫剂吡虫啉毒性中的应答特征。【方法】多重序列比对分析AmIscA的氨基酸序列特征和结构域组成;在线预测该蛋白的结构以及互作蛋白网络,预测它的生物学功能;构建系统进化树分析该蛋白的分类和进化关系;构建半致死模型,实时荧光定量PCR分析AmIscA基因在工蜂耐受杀虫剂吡虫啉毒性0~48h中的表达特征。【结果】AmIscA基因cDNA序列全长514bp,编码130个氨基酸残基,计算出AmIscA的相对分子质量为14.1kDa,pI为9.06。AmIscA为疏水性蛋白,具有铁硫簇组装蛋白保守的半胱氨酸和天冬氨酸残基,并且不具备信号肽,定位于线粒体中。经48h的半致死剂量吡虫啉暴露后的工蜂存活率为45%±5%。AmIscA基因表达受吡虫啉胁迫而上调,且呈先升高后下降趋势。【结论】AmIscA积极响应吡虫啉导致的毒性胁迫,可能通过抗氧化作用潜在地参与了意大利蜜蜂对吡虫啉毒性的耐受。 | 何彪 王悦弟 秦启倩 陈兰春 段佳欣 许金山 李治 | 2021 | 重庆师范大学学报(自然科学版)2021，38，4： | 0 |
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