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| 1 | 家蝇几丁质酶基因8(MDCht8)的cDNA克隆及功能初探显示文摘目的从家蝇的EST测序数据库中筛选获得家蝇几丁质酶基因8(MDCht8),对其进行分子特性分析,探讨Cht8基因在家蝇不同组织和发育时期的时空表达模式及其在家蝇免疫防御中的功能。方法克隆MDCht8基因,借助MEGA 5.0软件采用邻接法构建系统进化树;采用实时荧光定量PCR检测MDCht8基因时空表达模式及微生物诱导后不同时间点(3、6、12、18、24、36、48h)MDCht8基因表达水平的变化。结果 MDCht8基因的ORF框全长1 512bp,编码一个含503个氨基酸的蛋白多肽,理论相对分子质量约为56.801 7×10^3,进化树分析显示该蛋白与果蝇Cht8遗传距离较近。时空表达模式分析显示,MDCht8基因在2龄幼虫和3龄幼虫内表达量较高,家蝇3龄幼虫的各主要组织中以唾液腺和中肠中的表达水平较高。经白色念珠菌和大肠埃希菌诱导后,家蝇MDCht8基因出现明显上调,金黄色葡萄球菌诱导后未表现出明显上调。成功克隆了MDCht8基因,PCR扩增片段为1 512bp,与预期相符。结论初步揭示了MDCht8基因在家蝇不同虫期,不同组织表达量不同,提示几丁质酶基因在家蝇生长发育过程中具有重要作用;MDCht8基因在家蝇幼虫受到病原微生物攻击后表达上调,且攻击后的不同时间点表达量不同,提示MDCht8基因编码的几丁质酶参与家蝇的免疫防御过程。 | 吴沁怡 国果 赵文静 苏佩佩 杨隆兵 修江帆 | 2020 | 中国病原生物学杂志2020，15，2： | 1 |
| 2 | Knockdown of specific cuticular proteins analogous to peritrophin 3 genes disrupt larval and ovarian development in Bactrocera dorsalis(Diptera:Tephritidae)显示文摘Cuticular proteins(CPs)are critical components of the insect cuticle and play important roles in maintaining normal insect development and defense against various environmental stresses.The oriental fruit fly(Bactrocera dorsalis)is one of the most destructive pests worldwide,and its eight CPs analogous to peritrophin 3(BdCPAP3)family genes have been identified in our prev ious study.In the present study,we further explored the possible roles of CPAP3 genes in B.dorsalis development.Each sequence of BdCPAP3 genes contained three conserved ChtBD2(chitin-binding)domains.Spatial and temporal expression patterns revealed that the four BdCPAP3 genes(BdCPAP3-AI,B,E,and E2)might play important roles in larval pupariation of B.dorsalis.Moreover,treatment with a juvenile hormone analog(methoprene)significantly restricted expression of these four CPAP3 genes,whereas treatment with 20-hydroxy-ecdysone induced expression.The RNA interference(RNAi)results revealed that down-regulated CPAP3 genes led to signif-icant delay of pupariation,and injection of dsBdCPAP3-E into 5-d-old B.dorsalis larvae caused approximately 40%mortality.Interestingly,we also confirmed that BdCPAP3-D2 was involved in B.dorsalis ovarian development.This study showed that some specific CP4P3 genes had crucial roles in B.dorsalis development,and these CP genes could be used as potential targets to control this pest via RNAi. | Qiu-Li Hou Er-Hu Chen Wei Dou Jin-Jun Wang | 2021 | Insect Science2021，28，5： | 0 |
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