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| 1 | 脂质组学分析方法及其应用进展显示文摘脂质组学是一种基于高通量的测序技术,可有效研究脂类家族的各种脂质分子,进而阐明脂类物质在生物体中的代谢途径和调控机理。本研究综述了近几年脂质组学分析方法的优缺点及其应用进展,主要介绍了散弹枪脂质组学和基于色谱分离的质谱方法,为今后脂质组学的系统研究奠定基础。 | 韩梅 黄有军 | 2022 | 现代园艺2022，45，7： | 0 |
| 2 | Fast determination and quantification of triacylglycerols in plant oil by direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS)显示文摘Triacylglycerols(TGs)are the main constituents(95%-98%)of plant oil with high nutritional value.They are usually determined by high performance liquid chromatography coupled with mass spectrometry(HPLC-MS)which takes a relatively long time during chromatographic separation.Therefore,in this study,a method about fast determination and quantification of TGs in plant oil based on MALDI-TOF-MS(matrix-assisted laser desorption ionization time-of-flight mass spectrometry)was established.OCNT(oxidized carbon nanotube)and CNT(carbon nanotube)were firstly used as matrix of MALDI-TOF-MS for TGs identification.Satisfyingly,OCNT showed good ionization efficiency,especially when used with CHCA(α-Cyano-4-hydroxy cinnamic acid).Sandwich method was optimized to solve the problem that how matrix and sample were deposited to the plate well and to improve ionization efficiency.Nine calibration curves were established for TGs quantification analysis in plant oil.To verify the accuracy of this method,relative content(%)of fatty acids(FAs)calculated from TGs in peanut oil,rapeseed oil,and soybean oil,respectively,by MALDI-TOF-MS were compared with that calculated from FAMEs(fatty acid methyl esters)by gas chromatography.In general,the results were relatively consistent,proving that this method was convenient,fast and accurate for the determination of main TG molecular species in plant oil. | Xin Lv Fang Wei Hong Chen | 2019 | Oil Crop Science2019，4，4： | 0 |
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