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| 1 | Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity显示文摘The cellulase cocktail produced by marine Aspergillus niger exhibits a property of salt-tolerance,which is of great potential in cellulose degradation in high salt environment.In order to explain the mechanism on the salttolerance of the cellulase cocktail produced by marine A.niger,six cellulase components(AnCel6,AnCel7A,AnCel7B,AnEGL,AnBGL1 and AnBGL2)were obtained by directed expression.Studies on their enzymatic properties revealed that oneβ-glucosidase(AnBGL2)and one endoglucanase(AnEGL)exhibited an outstanding salttolerant property,and one cellobiohydrolase(AnCel7B)exhibited a certain salt-tolerant property.Subsequent study revealed that the salt-tolerant An EGL and AnCel7B endowed the cellulase cocktail with stronger salttolerant property,while the salt-tolerant An BGL2 had no positive effect.Moreover,after overexpression of AnCel6,AnCel7A,AnCel7B and AnEGL,the activity of cellulase cocktail increased by 80%,70%,63%and 68%,respectively.However,the activity of cellulase cocktail was not improved after overexpression of AnBGL1 and AnBGL2.After mixed-strain fermentation with cellobiohydrolase recombinants(cel6 a,cel7a and cel7b recombinants)and endoglucanase recombinant(egl recombinant),the the activity of cellulase cocktail increased by 114%,102%and91%,respectively. | Linian Cai Shengnan Xu Tao Lu Dongqiang Lin Shanjing Yao | 2020 | Chinese Journal of Chemical Engineering2020，28，4： | 2 |
| 2 | 松嫩平原盐碱地改良治理研究进展显示文摘松嫩平原是我国北方重要的农牧业基地,其西部是我国盐碱地主要分布区之一,属于典型内陆苏打盐碱地类型。由于土壤中含有大量可溶性盐,导致土壤理化性质恶化,抑制植物生长发育,严重影响农业生态环境和生产效益,有必要进行治理改良。盐碱地作为最重要的后备耕地资源,在转化为农业用地过程中具有潜力大的独特优势。采用物理、化学、水利工程和生物措施等针对盐碱地进行治理,可以将盐碱地转换为农业用地,由此扩大农业用地资源,从而保障国家粮食安全,改善区域农业生态环境。本文通过综述松嫩平原西部地区盐碱地治理研究,为我国盐碱地综合治理提供理论依据和决策参考,对于改善盐碱地区域生态环境,促进区域农业经济、社会和生态的绿色可持续发展具有重要意义。 | 王世睿 黄迎新 | 2023 | 土壤与作物2023，12，2： | 1 |
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