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| 1 | EGCG上调神经细胞自噬相关蛋白Beclin-1表达改善脊髓损伤后大鼠运动功能显示文摘目的观察表没食子儿茶素没食子酸酯(EGCG)对脊髓损伤(SCI)大鼠神经元自噬和相关调控蛋白Beclin-1表达的影响,探讨其SCI保护机制。方法 SD大鼠90只,分为假手术组、SCI组、EGCG组(n=25)。钳夹法建立SCI模型,EGCG组和SCI组损伤后即刻和1h后分别腹腔注射EGCG (50 mg/kg)和等量生理盐水,三组大鼠于术后不同时间1d、3d、1周、2周、3周、4周(n=5),分别进行斜板试验和运动功能(BBB)评分实验,测定各组大鼠的运动功能;用结晶紫(CV)染色法和免疫组化方法,观察SCI后6h、12h、24h、3d和7d时间内,从损伤中心到头端0~5.00 mm空间范围内,EGCG对脊髓神经元存活、自噬以及相关调控蛋白Beclin-1表达的影响。结果 SCI组在各个时间点斜板角度均明显小于假手术组,EGCG组相比于SCI组,除1d和3 d组外,其余1周~4周各个时间点斜板角度明显增加。SCI后各个时间点,SCI组评分明显低于假手术组,EGCG组相比于SCI组,术后3d~4周各个时间点BBB评分明显增加;SCI后6 h~7d,在0~0.50mm空间范围内均未观察到存活的神经元;在1.00~3.50mm距离内, EGCG组和SCI组相比,存活的神经元数明显增加,4.00mm处达峰值。SCI后6h~7d,在1.05~4.55mm范围内,EGCG组和SCI组相比,Beclin-1阳性表达的神经元数明显增加,其中在3d,2.55mm,Beclin-1表达达高峰,7d时减少。结论 EGCG干预改善脊髓损伤大鼠运动功能与上调神经元自噬蛋白Beclin-1表达相关。 | 廖琳丹 刁尧 王姝 杜补林 李雪娜 刘洁 李亚明 | 2019 | 解剖科学进展2019，25，2： | 7 |
| 2 | RNA sequencing screening of differentially expressed genes after spinal cord injury显示文摘In the search for a therapeutic schedule for spinal cord injury,it is necessary to understand key genes and their corresponding regulatory networks involved in the spinal cord injury process.However,ad hoc selection and analysis of one or two genes cannot fully reveal the complex molecular biological mechanisms of spinal cord injury.The emergence of second-generation sequencing technology(RNA sequencing)has provided a better method.In this study,RNA sequencing technology was used to analyze differentially expressed genes at different time points after spinal cord injury in rat models established by contusion of the eighth thoracic segment.The numbers of genes that changed significantly were 944,1362 and 1421 at 1,4 and 7 days after spinal cord injury respectively.After gene ontology analysis and temporal expression analysis of the differentially expressed genes,C5ar1,Socs3 and CCL6 genes were then selected and identified by real-time polymerase chain reaction and western blot assay.The mRNA expression trends of C5ar1,Socs3 and CCL6 genes were consistent with the RNA sequencing results.Further verification and analysis of C5ar1 indicate that the level of protein expression of C5ar1 was consistent with its nucleic acid level after spinal cord injury.C5ar1 was mainly expressed in neurons and astrocytes.Finally,the gene Itgb2,which may be related to C5ar1,was found by Chilibot database and literature search.Immunofluorescence histochemical results showed that the expression of Itgb2 was highly consistent with that of C5ar1.Itgb2 was expressed in astrocytes.RNA sequencing technology can screen differentially expressed genes at different time points after spinal cord injury.Through analysis and verification,genes strongly associated with spinal cord injury can be screened.This can provide experimental data for further determining the molecular mechanism of spinal cord injury,and also provide possible targets for the treatment of spinal cord injury.This study was approved ethically by the Laboratory Animal Ethics Committee of Jiangsu Province,China(approval No.2018-0306-001)on March 6,2018. | Yi Li Ying Chen Xiang Li Jian Wu Jing-Ying Pan Ri-Xin Cai Ri-Yun Yang Xiao-Dong Wang | 2019 | Neural Regeneration Research2019，14，9： | 4 |
| 3 | 脊柱微创术后椎旁肌缺血坏死2例显示文摘脊柱微创术(minimally invasive spine surgery,MISS)是脊柱外科发展进程中的必然,其中经皮椎弓根螺钉技术(percutaneous pedicle screw,PPS),通道辅助下微创经椎间孔腰椎椎体间融合术(MISTLIF),以其微小的创伤和术后迅速的康复被推崇。我科自2014年6月至2017年10月,采用PPS和通道辅助下MIS-TLIF完成246例患者,在取得满意疗效的同时,有2例患者术后出现了椎旁肌大范围的缺血坏死,在经过漫长治疗期,最终获得了痊愈,现报告总结如下。 | 薛文 王栋 杨阳 王增平 刘林 钱耀文 | 2018 | 中国骨伤2018，31，11： | 4 |
| 4 | RNA-Seq筛选脊髓损伤后的差异基因及部分基因功能分析显示文摘目的应用RNA-Seq技术分析脊髓损伤后不同阶段基因表达差异情况。方法使用IH脊髓打击仪制备脊髓损伤模型,SD大鼠48只随机分成两组,假手术组和实验组,实验组分别于损伤后1d、4d和7d取材。运用RNA-Seq技术筛选脊髓损伤后不同时间点的差异表达基因,对差异基因进行表达模式分析,功能注释,通路分析等,筛选出血红素氧化酶1(Hmox1)等感兴趣基因进行mRNA和蛋白水平的验证及功能分析。结果 RNA-Seq结果表明,与假手术组相比,脊髓损伤后1d、4d和7d发生显著变化的基因分别有944、1362和1421个。上述差异基因在损伤后不同时间点的变化趋势大致可分为8种形式。Real-time PCR结果显示,Hmox1、尿激酶型纤溶酶原激活剂(Plau)、纤溶酶原激活物抑制剂(Serpine1)和中性粒细胞胞质因子(Ncf2)的表达变化与RNA-Seq测序结果基本一致。Western blotting结果显示,脊髓损伤后Hmox1的蛋白表达变化趋势与核酸水平一致;免疫组织化学结果显示,损伤之后Hmox1主要表达于脊髓组织的神经元中。结论 RNA-Seq技术能高效分析脊髓损伤后的差异基因,从大量差异基因中筛选出感兴趣基因的验证和功能分析为阐述脊髓损伤的分子机制提供了部分资料。 | 陈颖 王迪 牛学英 王晓冬 陈雪 | 2017 | 解剖学报2017，48，2： | 1 |
| 5 | Changes in neurological and pathological outcomes in a modified rat spinal cord injury model with closed canal显示文摘Most animal spinal cord injury models involve a laminectomy,such as the weight drop model or the transection model.However,in clinical practice,many patients undergo spinal cord injury while maintaining a relatively complete spinal canal.Thus,open spinal cord injury models often do not simulate real injuries,and few previous studies have investigated whether having a closed spinal canal after a primary spinal cord injury may influence secondary processes.Therefore,we aimed to assess the differences in neurological dysfunction and pathological changes between rat spinal cord injury models with closed and open spinal canals.Sprague-Dawley rats were randomly divided into three groups.In the sham group,the tunnel was expanded only,without inserting a screw into the spinal canal.In the spinal cord injury with open canal group,a screw was inserted into the spinal canal to cause spinal cord injury for 5 minutes,and then the screw was pulled out,leaving a hole in the vertebral plate.In the spinal cord injury with closed canal group,after inserting a screw into the spinal canal for 5 minutes,the screw was pulled out by approximately 1.5 mm and the flat end of the screw remained in the hole in the vertebral plate so that the spinal canal remained closed;this group was the modified model,which used a screw both to compress the spinal cord and to seal the spinal canal.At 7 days post-operation,the Basso-Beattie-Bresnahan scale was used to measure changes in neurological outcomes.Hematoxylin-eosin staining was used to assess histopathology.To evaluate the degree of local secondary hypoxia,immunohistochemical staining and western blot assays were applied to detect the expression of hypoxia-inducible factor 1α(HIF-1α)and vascular endothelial growth factor(VEGF).Compared with the spinal cord injury with open canal group,in the closed canal group the Basso-Beattie-Bresnahan scores were lower,cell morphology was more irregular,the percentage of morphologically normal neurons was lower,the percentages of HIF-1α-and VEGF-immunoreactive cells were higher,and HIF-1αand VEGF protein expression was also higher.In conclusion,we successfully established a rat spinal cord injury model with closed canal.This model could result in more serious neurological dysfunction and histopathological changes than in open canal models.All experimental procedures were approved by the Institutional Animal Care Committee of Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,China(approval No.HKDL201810)on January 30,2018. | Xin Sun Xing-Zhen Liu Jia Wang Hai-Rong Tao Tong Zhu Wen-Jie Jin Kang-Ping Shen | 2020 | Neural Regeneration Research2020，15，4： | 1 |
| 6 | 丹曲林对急性脊髓损伤大鼠脊髓白质的保护作用显示文摘目的:探讨丹曲林(dantrolene,DA)对大鼠急性脊髓损伤后脊髓白质的保护作用。方法:采用钳夹法制作大鼠急性脊髓损伤模型(SCI)。将48只成年雄性Sprague—Dawley大鼠体重280~310g,随机分成3组,每组16只,假手术组(Sham组):只行椎板切除术;SCI组:术前1d腹腔注射生理盐水;丹曲林处理组(DA组):术前1d腹腔注射10ms/kg丹曲林。SCI损伤后3、7、14、21、28d利用BBB评分量表评估大鼠后肢运动功能恢复情况;术后24h,利用抗NF200单克隆抗体的免疫荧光组织化学法标记轴突,观察各组轴突的形态和数量变化;WesternBlot方法检测各组脊髓损伤部位的髓鞘碱性蛋白(Myelin basic protein,MBP)和环核苷酸磷酸二酯酶(2’,3’-cyclic nucleotide phosphodiesterase,CNPase)的蛋白表达水平。结果:不同时间点SCI组大鼠的BBB评分显著低于Sham组,提示SCI模型制备成功。注射丹曲林后,不同时间点SCI动物的BBB评分明显升高(P〈0.05),提示丹曲林可促进大鼠后肢运动功能的恢复。NF200免疫荧光组织化学法显示:Sham组轴突长,排列有序、分布均匀;SCI后24h,NF200’纤维断裂、数量减少、排列紊乱、部分甚至卷曲;给予丹曲林后,NF200+纤维数量显著增多且较长,排列较规则。Western Blot结果显示:SCI组CNPase,MBP蛋白的表达量较Sham组显著降低(P〈0.01),给予丹曲林后可明显升高两者的表达(P〈0.01)。结论:丹曲林对急性脊髓损伤大鼠白质具有一定的保护作用。 | 瓮调调 廖博 毕一鸣 史明 曾联婷 赵永 付炜 韩军良 | 2016 | 神经解剖学杂志2016，32，6： | 1 |
| 7 | 完全性脊髓损伤急诊手术:安全或有效?显示文摘脊髓损伤的手术时机是一个复杂且存在较大争议的问题,全身和局部(也就是神经功能)的安全性和有效性决定了脊柱脊髓损伤的手术时机。《伴完全性脊髓损伤胸腰椎骨折急诊手术的临床意义》一文(下称该文)对胸腰段完全性脊髓损伤进行了急诊手术治疗的有益尝试,工作值得肯定。笔者从三个方面谈谈自己的观点。 | 孙天胜 | 2015 | 中华创伤杂志2015，31，6： | 0 |
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