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| 1 | Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling fourdimensional separation and characterization of the multicomponents from white ginseng and red ginseng显示文摘Inherent complexity of plant metabolites necessitates the use of multi-dimensional information to accomplish comprehensive profiling and confirmative identification.A dimension-enhanced strategy,by offline two-dimensional liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry(2 D-LC/IM-QTOF-MS)enabling four-dimensional separations(2 D-LC,IM,and MS),is proposed.In combination with in-house database-driven automated peak annotation,this strategy was utilized to characterize ginsenosides simultaneously from white ginseng(WG)and red ginseng(RG).An offline 2 DLC system configuring an Xbridge Amide column and an HSS T3 column showed orthogonality 0.76 in the resolution of ginsenosides.Ginsenoside analysis was performed by data-independent high-definition MSE(HDMSE)in the negative ESI mode on a Vion?IMS-QTOF hybrid high-resolution mass spectrometer,which could better resolve ginsenosides than MSEand directly give the CCS information.An in-house ginsenoside database recording 504 known ginsenosides and 58 reference compounds,was established to assist the identification of ginsenosides.Streamlined workflows,by applying UNIFI?to automatedly annotate the HDMSEdata,were proposed.We could separate and characterize 323 ginsenosides(including 286 from WG and 306 from RG),and 125 thereof may have not been isolated from the Panax genus.The established 2 D-LC/IM-QTOF-HDMSEapproach could also act as a magnifier to probe differentiated components between WG and RG.Compared with conventional approaches,this dimensionenhanced strategy could better resolve coeluting herbal components and more efficiently,more reliably identify the multicomponents,which,we believe,offers more possibilities for the systematic exposure and confirmative identification of plant metabolites. | Tiantian Zuo Chunxia Zhang Weiwei Li Hongda Wang Ying Hu Wenzhi Yang Li Jia Xiaoyan Wang Xiumei Gao Dean Guo | 2020 | Journal of Pharmaceutical Analysis2020，10，6： | 9 |
| 2 | Panax japonicus and chikusetsusaponins: A review of diverse biological activities and pharmacology mechanism显示文摘Panax japonicus, which in the Tujia dialect is known as 'Baisan Qi' and 'Zhujieshen', is a classic 'qi' drug of Tujia ethnomedicine and it has unique effects on disease caused by 'qi' stagnation and blood stasis.This paper serves as the basis of further scientific research and development of Panax japonicus. The pharmacology effects of molecular pharmacology were discussed and summarized. P. japonicus plays an important role on several diseases, such as rheumatic arthritis, cancer, cardiovascular agents, and this review provides new insights into P. japonicus as promising agents to substitute ginseng and notoginseng. | Xiao-Juan Wang Qian Xi Yang Liu Sai Jiang Wei Li Bin Li Wei Wang Chang-Xiao Liu | 2021 | Chinese Herbal Medicines2021，13，1： | 3 |
| 3 | 基于数据挖掘和整合药理学平台牛膝芍药治疗骨关节炎成分-靶标-通路分析显示文摘目的以牛膝-白芍药对为研究对象,借助整合药理学平台(www.tcmip.cn)探究其对骨关节炎的作用机制。方法利用整合药理学平台将牛膝-白芍的化学成分进行靶标预测,与关节炎(osteoarthritis,OA)相关疾病靶标信息进行蛋白-蛋白相互作用(PPI)网络构建,富集分析该药对治疗OA的关键靶标,构建牛膝-白芍药对化学成分、关键作用靶标和疾病相关通路的相互作用网络,绘制'中药-化学成分-关键靶标-通路'的可视化多维网络,结合相关文献报道对牛膝-白芍药对治疗OA的作用机制进行预测。结果预测牛膝化学成分15种,包括多糖类、皂苷类、甾酮类、黄酮类等。预测白芍化学成分50种,包括苷类、萜类、黄酮类、鞣质类等。其中药物和疾病的共同作用靶标PRKAA1、PRKAA2、PRKAB1、NF-KB1、NF-KB2、NF-KB1A、CXCR1、BCL2、TP53。其中疾病通路包括内分泌系统、前列腺癌、乙型肝炎、甲状腺激素、癌症、循环系统、神经系统、神经营养因子、细胞生长和死亡、FoxO信号通路等。结论在整合药理学平台的基础上,该研究初步揭示了牛膝-白芍药对治疗OA的潜在活性成分和可能作用机制,为进一步探究牛膝-白芍治疗骨关节炎作用机制提供了理论支持和指导。 | 杨永菊 杨国元 于河山 关雪峰 | 2020 | 辽宁中医药大学学报2020，22，3： | 3 |
| 4 | 虎潜丸治疗骨质疏松症研究进展显示文摘骨质疏松症(Osteoporosis,OP)具有高患病率、高致残率,对中国中老年人生命安全及生活质量造成了极大的威胁,寻求安全有效的治疗方案防治OP具有重大的临床意义。近年来研究表明,虎潜丸临床治疗骨质疏松症效果显著,但其作用机制尚未完全明确。综述近几年来有关虎潜丸的文献报道,从虎潜丸组方中单味中药及虎潜丸组方应用于治疗骨质疏松症的药理作用机制研究、虎潜丸临床应用研究3个方面进行探讨总结,以期为临床诊疗提供思路。 | 姚鹏程 马斌祥 关永林 孙乐伟 张洋 邓梦 常斌 | 2022 | 中医药临床杂志2022，34，6： | 1 |
| 5 | Deep chemical identification of phytoecdysteroids in Achyranthes bidentata Blume by UHPLC coupled with linear ion trap-Orbitrap mass spectrometry and targeted isolation显示文摘Achyranthes bidentata Blume is widely used as a traditional Chinese medicine with the effects of nourishing the liver and kidneys and strengthening muscles and bones.In this work,a rapid and simple strategy was developed for characterizing phytoecdysteroids by ultra-high-performance liquid chromatography coupled with liner ion trap-Orbitrap mass spectrometry using electrospray ionization in the negative mode.As a result,47 phytoecdysteroids were unambiguously or tentatively characterized.Among them,seven known compounds were identified according to the reference standards along with molecular formula,retention time and fragmentation patterns,while others were mostly potential new compounds.Through targeted isolation,the structures of three new compounds were determined by NMR spectra,which were consistent with LC-MS characterization.The present study provides an efficient method to deeply characterize phytoecdysteroids. | WANG Ying-Ying LI Jia-Yuan YAO Chang-Liang ZHANG Jian-Qing YU Yang YAO Shuai GAO Min WU Shi-Fei WEI Wen-Long BI Qi-Rui GUO De-An | 2022 | Chinese Journal of Natural Medicines2022，20，7： | 0 |
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