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| 1 | 青春期排卵障碍性异常子宫出血危险因素及治疗方法显示文摘目的分析青春期排卵障碍性异常子宫出血危险因素及治疗方法。方法选取2015年1月-2019年3月对患有子宫异常出血的青春期女生90例进行问卷调查,随机数字表法分成对照组45例和观察组45例,对选取治疗的病例所得调查问卷结果进行单因素和多因素分析,找出青春期女生异常子宫出血的危险因素,通过采用单独的西医治疗采用口服达英-35和中西医治疗在对照组的基础上联合使用补肾固冲汤,对比两种治疗方法的效果。结果青春期排卵障碍性异常子宫出血危险因素有患者的母亲有子宫异常出血史、学习压力大、睡眠状况不佳、经期不注重饮食导致身体受凉等。采用口服达英-35联合使用补肾固冲汤的观察组患者症状改善比采用口服达英-35的对照组明显,复发情况减少,治疗效果显著,差异有统计学意义(P<0.05)。结论青春期排卵障碍性异常子宫出血的主要危险因素包括青春期女生母亲功血史、学习压力、饮食和睡眠情况等;对于这种疾病的治疗采用中西医结合的方式比单独的西医治疗更为有效。 | 申净美 郑燕红 宋丹丹 胡超峰 | 2021 | 中国妇幼保健2021，36，7： | 14 |
| 2 | 基于LC-Q/TOF-MS代谢组学方法筛选奶牛妊娠识别阶段乳样生物标志物的研究显示文摘本研究旨在筛选奶牛妊娠识别阶段乳汁生物标志物,为早期妊娠诊断提供新的思路和方法。选择胎次和体况相近的荷斯坦健康高产奶牛10头,同期发情后,采集人工授精当天和第17天乳样,采用高效液相色谱-四极杆-飞行时间质谱(LC-Q/TOF-MS)代谢组学技术,结合主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLSDA)对两组乳样代谢轮廓进行模式识别;根据OPLS-DA模型中的VIP值(VIP>1)和Kruskal-Wallis检验的P值(P<0.05)筛选两组乳样差异代谢物;ROC曲线进一步考察这些差异代谢物对两组乳样分类的识别能力。结果表明:1)人工授精当天与第17天乳样代谢轮廓存在明显差异;2)10个代谢物(甲羟戊酸内酯、色胺、L-酪氨酸、磷酸二氢新蝶呤、乙酰半胱氨酸、N-乙酰基-L-酪氨酸、L-脯氨酸、四氢1-苯甲基-1,2,3,4-四氢异喹啉、鸟苷、十七烷基肉碱)被初步确定为两组乳样的差异代谢物;3)这10个差异代谢物中仅有6个代谢物(L-酪氨酸、N-乙酰基-L-酪氨酸、L-脯氨酸、四氢1-苯甲基-1,2,3,4-四氢异喹啉、鸟苷和十七烷基肉碱)对两组乳样具有显著的区分能力,最终被视为妊娠识别阶段潜在乳样生物标志物。综上表明,牛奶中L-酪氨酸、N-乙酰基-L-酪氨酸、L-脯氨酸、四氢1-苯甲基-1,2,3,4-四氢异喹啉、鸟苷和十七烷基肉碱有望成为妊娠识别期妊娠诊断指标,也可为深入研究奶牛早期妊娠识别机理提供新的视角。 | 郭延生 陶金忠 | 2018 | 畜牧兽医学报2018，49，8： | 10 |
| 3 | hCG日高孕酮对卵巢正常反应者子宫内膜容受性相关基因表达影响研究显示文摘目的探讨人绒毛膜促性腺激素(hCG)注射日高孕酮(P)对卵巢正常反应者种植窗期子宫内膜基因表达变化的影响。方法选取2014年4月至2015年12月在郑州大学第二附属医院生殖医学中心行长方案体外受精-胚胎移植(IVF-ET)助孕卵巢正常反应患者12例,该次取卵周期取消移植。按hCG日P水平分组:高P组6例(P≥7.13nmol/L);P未升高组6例(P<7.13nmol/L)。取卵后5d采集子宫内膜组织,RNA-seq分析两组内膜基因表达变化,筛选差异表达基因,qRT-PCR验证差异基因,Western blot检测差异基因产物蛋白水平表达,分析相关基因功能。结果两组之间共有31个显著性差异基因。qRT-PCR验证,其中SLC7A4、DDX5、IFI6、HLA-DRB4、HLADRB5、SCGB1D2与RNA-seq结果一致。Western blot结果显示,高P组中SLC7A4、IFI6、HLA-DRB4、HLA-DRB5蛋白表达量低于P未升高组,而DDX、SCGB1D2蛋白表达量高于P未升高组。结论hCG日高P可能影响了种植窗期子宫内膜容受性相关基因表达,为进一步探索其具体分子作用机制提供相应基因靶点。 | 李朋粉 谭丽 马丽影 赵冬梅 项云改 张丹 卢娜 禹果 | 2020 | 中国实用妇科与产科杂志2020，36，6： | 9 |
| 4 | Perturbations in the uterine luminal fluid composition are detrimental to pregnancy establishment in cattle显示文摘Background: A major,unresolved issue is how the uterine microenvironment determines pregnancy success in cattle.Before implantation,conceptus development depends on the uterine secretome(i.e.,histotroph).Despite its pivotal role,little is known about the dynamics of histotroph synthesis and changes in composition throughout the early diestrus and the relevance to pregnancy establishment.We hypothesize that disturbances on histotroph composition affect the establishment of pregnancy.Aim was to disturb histotroph composition at early diestrus and verify the effects on:(Exp.1) timing to restore its composition; and(Exp.2) pregnancy rate after multiple-embryo transfer.Estrous cycle of multiparous Nelore cows were synchronized and estrus was considered d 0(D0) of the experiments.Disturbance was through flushing each uterine horn with 30 m L of DMPBS and collecting the resulting uterine luminal flushing(ULF) on D1; D4; D7; D1 + D4 + D7.Control group remained not-collected.In Exp.1,ULF was collected on D7.5 from all animals and used for quantification of total protein concentration and abundance of albumin.In Exp.2,three in vitro-produced embryos were transferred to the uterine horn ipsilateral to the ovary containing the CL on D7.5 and pregnancy was checked on D25 by ultrasound.Results: In Exp.1,ULF collection on D4 or D7 increased(1.5-to 2.2-folds) the total protein concentration and albumin abundance.ULF collection on D1 did not alter(P > 0.10) these endpoints.In Exp.2,ULF collected on D4 or D7 decreased pregnancy rates to approximately half of that measured in the remaining groups.Conclusions: Subtle perturbations imposed to the native intrauterine milieu,such as those caused by a single,low-volume collection of ULF,profoundly disturbs intrauterine composition and pregnancy success.At least 4 d were necessary for the uterus to recover its composition and the functional capacity to carry post-implantation gestation. | Thiago Martins Guilherme Pugliesi Mariana Sponchiado Angela M.Gonella-Diaza Oscar A.Ojeda-Rojas Frederich D.Rodriguez Roney S.Ramos Andrea C.Basso Mario Binelli | 2019 | Journal of Animal Science and Biotechnology2019，10，1： | 0 |
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