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| 1 | RNA沉默转基因技术在植物抗病虫害研究中的作用显示文摘RNA沉默技术由于其简便、高效及高特异性在各种模式和非模式生物系统中得到了广泛的应用。植物转基因技术是将植物、动物或微生物中分离到的目的基因,通过各种手段整合到植物基因组上,使其稳定遗传并赋予植物新的优良性状的生物技术。RNA沉默在植物转基因工程领域的研究十分广泛,目前已经成功研究培育出许多抗病毒、抗虫转基因植物,为农业病虫害的防控提供了新的思路和方法。本文重点综述了近年来植物转基因介导RNA沉默在抗虫与抗病毒研究方面的进展,加深人们对转基因植物抗虫与抗病毒研究的认识。 | 杨美玲 李琦 陈佳琦 张晓明 | 2020 | 植物保护2020，46，4： | 5 |
| 2 | RNAi递送系统在害虫防治中的研究进展显示文摘RNAi是由dsRNA引发的,靶向目的基因的高效与特异的基因沉默技术。由于其高效性、特异性和便捷性,RNAi技术广泛应用于基因功能研究、高通量目的基因筛选、基因治疗、药物靶标预测和农业病虫害防治等领域。考虑到RNAi效率、安全性和预期靶基因下调的潜在障碍,RNAi提供高效防治应用的前提是以合适的方式递送效应RNA。本文对近期农业重要性害虫和病媒害虫防治应用中开发的RNAi递送系统进行综述,对这些RNAi递送系统的干扰效率和多重进入位点作了比较,并展望了RNAi递送系统在害虫防治中的应用,以期更好地完善RNAi技术在昆虫学研究和害虫防治中的应用。 | 肖蓓 幸诗斯 邓丹 彭威 翟宗昭 | 2021 | 应用昆虫学报2021，58，4： | 4 |
| 3 | RNA interference-mediated silencing of vATPase subunits A and E affect survival and development of the 28-spotted ladybeetle,Henosepilachna vigintioctopunctata显示文摘RNA interference(RNAi)has emerged as a powerful tool for developing novel management strategies for controlling insect pests.The 28-spotted ladybeetle,Henosepilachna vigintioctopunctata is one of the most important pests attacking solanaceous plants in Asia.In this study,the potential of dietary RNAi to manage H.vigintioctopunctata was investigated using both in vitro synthesized and bacterially expressed double-stranded RNAs(dsRNAs)of HvvATPase A and HvvATPase E.The expression levels of HvvATPase A and HvvATPase E were higher in Malpighian tubules than in other tissue types.The silencing of HvvATPase A and HvvATPase E led to significant mortality in H.vigintioctopunctata larvae.In addition,the ingestion of HvvATPase A and HvvATPase E significantly deterred feeding behavior and subsequently arrested the development of H.vigintioctopunctata.Notably,the bacterially expressed dsRNAs consistently caused higher mortality in larvae and adults.Finally,the nontarget effects of the dsRNAs of H.vigintioctopunctata on the predatory ladybeetle Propylaea japonica were evaluated.P.japonica 1st instar larvae were administered vATPase A and vATPase E dsRNAs from H.vigintioctopunctata and P.japonica under the worst-case scenario,in which dsGFP served as negative control.There were significant effects of dsHvvATPase A on P.japonica at the transcriptional level but not at the organismal level,whereas dsHvvATPase E did not effect P.japonica at either the transcriptional or the organismal level.Collectively,the results of the study suggest that HvvATPase A and HvvATPase E can act as novel molecular targets for the control of H.vigintioctopunctata. | Wei Guo Mujuan Guo Chunxiao Yang Zhuoqi Liu Shimin Chen Jing Lu Baoli Qiu Youjun Zhang Xuguo Zhou Huipeng Pan | 2021 | Insect Science2021，28，6： | 3 |
| 4 | Insect resistance management in Bacillus thuringiensis cotton by MGPS(multiple genes pyramiding and silencing)显示文摘The introduction of Bacillus thuringiensis(Bt)cotton has reduced the burden of pests without harming the environment and human health.However,the efficacy of Bt cotton has decreased due to field-evolved resistance in insect pests over time.In this review,we have discussed various factors that facilitate the evolution of resistance in cotton pests.Currently,different strategies like pyramided cotton expressing two or more distinct Bt toxin genes,refuge strategy,releasing of sterile insects,and gene silencing by RNAi are being used to control insect pests.Pyramided cotton has shown resistance against different cotton pests.The multiple genes pyramiding and silencing(MGPS)approach has been proposed for the management of cotton pests.The genome information of cotton pests is necessary for the development of MGPS-based cotton.The expression cassettes against various essential genes involved in defense,detoxification,digestion,and development of cotton pests will successfully obtain favorable agronomic characters for crop protection and production.The MGPS involves the construction of transformable artificial chromosomes,that can express multiple distinct Bt toxins and RNAi to knockdown various essential target genes to control pests.The evolution of resistance in cotton pests will be delayed or blocked by the synergistic action of high dose of Bt toxins and RNAi as well as compliance of refuge requirement. | ZAFAR Muhammad Mubashar RAZZAQ Abdul FAROOQ Muhammad Awais REHMAN Abdul FIRDOUS Hina SHAKEEL Amir MO Huijuan REN Maozhi | 2020 | Journal of Cotton Research2020，3，4： | 0 |
| 5 | VIGS技术在昆虫基因功能研究中的应用显示文摘RNAi作为一种基因沉默的分子生物学技术,广泛应用于生物基因功能研究。在昆虫RNAi研究中,病毒诱导的基因沉默(Virus-induced gene silencing,VIGS)技术是一种将dsRNA导入昆虫体内的独特策略。目前,这种方法在半翅目和鳞翅目昆虫的研究中有诸多报道。通过携带靶标昆虫基因片段的重组病毒载体侵染寄主植物,病毒在复制过程中形成dsRNA。实验昆虫取食被侵染的寄主植物后会摄入大量的dsRNA,从而达到dsRNA导入的目的。与其它dsRNA导入方法相比,VIGS技术具有高效、高通量、昆虫接受度高等优势。本文综合分析了VIGS技术的作用机理、应用研究、影响效率的因素及其优缺点,将来应围绕这些问题进一步深化该技术在昆虫学研究中的应用。 | 王鑫仪 靖湘峰 | 2021 | 应用昆虫学报2021，58，3： | 0 |
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