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| 1 | TNF-α suppresses sweat gland differentiation of MSCs by reducing FTO-mediated m^6 A-demethylation of Nanog mRNA显示文摘An effect of inhibition of tumor necrosis factor-α(TNF-α)on differentiation of mesenchymal stromal cells(MSCs)has been demonstrated,but the exact mechanisms that govern MSCs differentiation remain to be further elucidated.Here,we show that TNF-αinhibits the differentiation of MSCs to sweat glands in a specific sweat gland-inducing environment,accompanied with reduced expression of Nanog,a core pluripotency factor.We elucidated that fat mass and obesity-associated protein(FTO)-mediated m^6 A demethylation is involved in the regulation of MSCs differentiation potential.Exposure of MSCs to TNF-αreduced expression of FTO,which demethylated Nanog m RNA.Reduced expression of FTO increased Nanog m RNA methylation,decreased Nanog m RNA and protein expression,and significantly inhibited MSCs capacity for differentiation to sweat gland cells.Our finding is the first to elucidate the functional importance of m^6 A modification in MSCs,providing new insights that the microenvironment can regulate the multipotency of MSCs at the post-transcriptional level.Moreover,to maintain differentiation capacity of MSCs by regulating m^6 A modification suggested a novel potential therapeutic target for stem cellmediated regenerative medicine. | Yihui Wang Rui Wang Bin Yao Tian Hu Zhao Li Yufan Liu Xiaoli Cui Liuhanghang Cheng Wei Song Sha Huang Xiaobing Fu | 2020 | Science China(Life Sciences)2020，63，1： | 9 |
| 2 | 具核梭杆菌感染的巨噬细胞对人舌鳞癌细胞系Cal-27细胞增殖与迁移的影响显示文摘目的:观察具核梭杆菌(F.nucleatum)诱导的巨噬细胞对人舌鳞癌细胞系Cal-27细胞增殖、迁移的影响。方法:F.nucleatum以感染复数(MOI)100∶1,刺激THP-1细胞来源的巨噬细胞,24 h后收集细胞条件培养液,以THP-1细胞及THP-1细胞来源巨噬细胞的条件培养液为对照。将获得的各组条件培养液与Cal-27细胞共培养,CCK-8法检测各组细胞增殖水平的变化,划痕实验检测Cal-27细胞迁移能力的变化。ELISA检测各组条件培养液中肿瘤坏死因子α(TNF-α)及白细胞介素(IL)-6表达水平。结果:与2个对照组相比,F.nucleatum诱导的巨噬细胞培养液上清使Cal-27细胞增殖减少(P<0.05),相对迁移率增高(P<0.05)。F.nucleatum刺激巨噬细胞后,条件培养液中TNF-α和IL-6水平均较刺激前明显增加(P<0.05)。结论:F.nucleatum感染能促进巨噬细胞分泌TNF-α和IL-6,进而可能促进Cal-27细胞迁移,抑制其增殖。 | 方媛春 孙颖 胡永 | 2021 | 口腔生物医学2021，12，3： | 1 |
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