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| 1 | 外泌体提取及保存技术研究进展显示文摘外泌体是多种活细胞经过'内吞–融合–外排'等一系列过程主动向胞外分泌的纳米级双层膜结构小囊泡,广泛存在于血液和尿液等生物体液中。因其携带着多种蛋白质、核酸和脂质等生物活性分子,所以外泌体不仅在细胞间物质交换和信息传递中发挥重要作用,而且对疾病诊断、预后预测和治疗管理等均具有提示意义。外泌体的高效提取、分离和完整保存是研究其在机体内生物学作用和功能的重要前提,也是制约基于外泌体的临床检测技术和治疗载体技术的关键。该综述将针对目前国内外外泌体提取和保存领域的最新研究进展加以综述,并对其特点进行对比和分析,以促进外泌体研究方法的标准化,以及相关技术的研发和应用拓展。 | 韩杰 葛安 马晓霞 王佳月 杨彩婷 董丽 | 2021 | 中国细胞生物学学报2021，43，2： | 6 |
| 2 | 外泌体分离方法研究进展显示文摘外泌体是一种由细胞分泌的密度在1.18~1.19 kg/L纳米级膜性囊泡,它在生物进程中扮演者重要的角色,如信息传递、运输载体、抗原呈递等。近年来,外泌体在研究界引起了相当大的关注。一方面科学家及医疗工作者致力于寻找新的以外泌体为基础的生物标志物进行健康监测及医疗诊断;另一方面,现阶段缺乏技术标准化方法来保证分离出高产量、高纯度的外泌体,这为实现可靠的生物标志物的发现制造了障碍。本文综述了外泌体分离方法相关研究领域的进展,特别在分离机制、适用范围及应用前景等方面。最后,本文讨论了基于微流控技术的外泌体分离方法的新进展,并肯定了微流控技术在外泌体分离领域的重要性。 | 张玉星 张响 陈文杰 张丽果 | 2018 | 转化医学电子杂志2018，5，4： | 6 |
| 3 | The mini player with diverse functions:extracellular vesicles in cell biology,disease,and therapeutics显示文摘Extracellular vesicles(EVs)are tiny biological nanovesicles ranging from approximately 30–1000 nm in diameter that are released into the extracellular matrix of most cell types and in biofluids.The classification of EVs includes exosomes,microvesicles,and apoptotic bodies,dependent on various factors such as size,markers,and biogenesis pathways.The transition of EV relevance from that of being assumed as a trash bag to be a key player in critical physiological and pathological conditions has been revolutionary in many ways.EVs have been recently revealed to play a crucial role in stem cell biology and cancer progression via intercellular communication,contributing to organ development and the progression of cancer.This review focuses on the significant research progress made so far in the role of the crosstalk between EVs and stem cells and their niche,and cellular communication among different germ layers in developmental biology.In addition,it discusses the role of EVs in cancer progression and their application as therapeutic agents or drug delivery vehicles.All such discoveries have been facilitated by tremendous technological advancements in EV-associated research,especially the microfluidics systems.Their pros and cons in the context of characterization of EVs are also extensively discussed in this review.This review also deliberates the role of EVs in normal cell processes and disease conditions,and their application as a diagnostic and therapeutic tool.Finally,we propose future perspectives for EV-related research in stem cell and cancer biology. | Abhimanyu Thakur Xiaoshan Ke Ya-Wen Chen Pedram Motallebnejad Kui Zhang Qizhou Lian Huanhuan Joyce Chen | 2022 | Protein & Cell2022，13，9： | 3 |
| 4 | 外泌体提取方法及其miRNA在非小细胞肺癌中的应用显示文摘作为癌症诊断有前景的生物学标志物,外泌体在癌症的液体活检中受到很大关注。外泌体的生物学价值和临床应用价值凸显了开发有效提取和分离外泌体技术的重要性和必要性。外泌体miRNA越来越被认为是潜在的疾病非侵入性生物学标志物,有越来越多的证据表明外泌体miRNA表达失调与非小细胞肺癌的发生和发展相关。该文对几种不同外泌体分离提取方法及其miRNA在非小细胞肺癌中的作用进行综述。 | 吴静(综述) 沈佐君(审校) | 2020 | 国际检验医学杂志2020，41，15： | 2 |
| 5 | 离心微流控芯片技术用于核酸等温扩增的研究进展显示文摘常规聚合酶链式反应对反应条件的要求很高,需要在多个温度下进行循环,而核酸等温扩增仅需在恒定温度下就能进行核酸的高效快速扩增。离心式微流控芯片技术具有微型化、集成化、高通量、自动化等优势,可实现实时、低成本生化检测分析。本文介绍了离心微流控芯片技术在核酸扩增前处理和核酸等温扩增中的应用,主要包括核酸提取、试剂预存储和核酸等温扩增等方面,并且对离心微流控芯片技术用于核酸等温扩增中存在的问题与未来的发展方向进行分析讨论。 | 曹宁 周新丽 | 2020 | 工业微生物2020，50，6： | 2 |
| 6 | 外泌体应用于膀胱癌诊疗的基础与临床研究进展显示文摘膀胱癌是泌尿系统最常见的恶性肿瘤,其术后复发和进展一直是临床关注的焦点问题。外泌体是由细胞内多囊泡体与细胞膜融合后释放到细胞外基质中的膜性囊泡。通过携带DNA、RNA、miRNA、非编码RNA和蛋白质等,实现细胞间生物信息的交换和传递。近来的研究表明外泌体参与了膀胱癌的侵袭、转移及进展过程,文章就外泌体应用于膀胱癌诊断和治疗的基础和临床研究进展进行综述。 | 刘维辉 陈俊毅 李毅宁 朱绍兴 | 2019 | 中国肿瘤2019，28，1： | 1 |
| 7 | Small extracellular vesicles in cancer显示文摘Extracellular vesicles(EV)are lipid-bilayer enclosed vesicles in submicron size that are released from cells.A variety of molecules,including proteins,DNA fragments,RNAs,lipids,and metabolites can be selectively encapsulated into EVs and delivered to nearby and distant recipient cells.In tumors,through such intercellular communication,EVs can regulate initiation,growth,metastasis and invasion of tumors.Recent studies have found that EVs exhibit specific expression patterns which mimic the parental cell,providing a fingerprint for early cancer diagnosis and prognosis as well as monitoring responses to treatment.Accordingly,various EV isolation and detection technologies have been developed for research and diagnostic purposes.Moreover,natural and engineered EVs have also been used as drug delivery nanocarriers,cancer vaccines,cell surface modulators,therapeutic agents and therapeutic targets.Overall,EVs are under intense investigation as they hold promise for pathophysiological and translational discoveries.This comprehensive review examines the latest EV research trends over the last five years,encompassing their roles in cancer pathophysiology,diagnostics and therapeutics.This review aims to examine the full spectrum of tumor-EV studies and provide a comprehensive foundation to enhance the field.The topics which are discussed and scrutinized in this review encompass isolation techniques and how these issues need to be overcome for EV-based diagnostics,EVs and their roles in cancer biology,biomarkers for diagnosis and monitoring,EVs as vaccines,therapeutic targets,and EVs as drug delivery systems.We will also examine the challenges involved in EV research and promote a framework for catalyzing scientific discovery and innovation for tumor-EV-focused research. | Komal Abhange Amy Makler Yi Wen Natasha Ramnauth Wenjun Mao Waseem Asghar Yuan Wan | 2021 | Bioactive Materials2021，6，11： | 1 |
| 8 | 单细胞转录组测序在家畜生产中的应用显示文摘常规转录组学研究大多是在器官或组织水平上进行转录组测序,忽略了细胞间基因表达的异质性,而单细胞转录组测序(single cell RNA-seq, scRNA-seq)技术可从动物组织分离出的单个细胞的微量全转录组RNA扩增后进行高通量测序,从而在单细胞水平上揭示基因与表现型的关系。现概述scRNA-seq的关键技术要点,并根据其本身所具有的技术优势,基于家畜相关研究的特点分析该技术在家畜研究中应用的适用性,重点综述现阶段scRNA-seq技术在家畜中应用的研究进展,以期为家畜相关研究提供方法指导和理论依据。 | 夏银钊 何天乐 陈俊材 唐志如 杨震国 | 2022 | 中国兽医学报2022，42，2： | 1 |
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