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| 1 | 固体微生物菌剂在克拉玛依石油污染土壤生物修复中的应用显示文摘分析了克拉玛依石油污染土壤的理化性质,采用固体微生物菌剂对该土壤进行生物修复,考察了最优修复条件及修复过程中土壤微生物数量、酶活性和石油烃组分的变化。结果表明,克拉玛依石油污染土壤是以粉砂为主的灰漠土,含水率低,含油率高,弱碱性,土壤中三大营养元素(氮、磷、钾)的有效含量低,不利于微生物的生长繁殖。最优修复条件为土壤孔隙度55%、含水率25%、固体菌剂添加量5%、氮/磷摩尔比10、生物表面活性剂添加量0.5%,在此条件下经过60d的生物修复,含油率由最初的4.07%下降到1.81%,降解率为55.53%,小于C_(27)的正构烷烃得到了明显的降解,土壤中的微生物数量、酶活性(脱氢酶活性、过氧化氢酶活性和多酚氧化酶活性)均有所提高。在生物修复过程中,单靠改善外在环境条件进行生物刺激,无法有效去除石油烃,添加微生物菌剂进行生物强化是去除土壤中石油类污染物的关键因素。 | 李政 顾贵洲 宁春莹 李法云 杨磊 | 2016 | 石油学报(石油加工)2016，32，6： | 19 |
| 2 | 海洋石油降解菌的筛选及复合菌系的构建显示文摘为采用生物法治理海洋石油污染,以原油为唯一碳源,从深圳海域5个采样点取样,通过富集、涂布平板分离高效石油降解菌,并以复配、正交等方式构建石油降解复合菌系;通过生理生化实验和16S r RNA基因序列分析对菌株进行鉴定;采用单因素实验对复合菌系降解石油的条件进行优化,并使用气相色谱-质谱法(GC-MS)研究其对石油的降解特性。结果显示,共分离得到22株高效石油降解菌,对石油的降解率为34.5%-52.2%;由S1-30、S1-38和S2-13构建了复合菌系SQ1,对石油降解率可达68.3%,3株菌分别鉴定为棒杆菌(Corynebacterium sp.)、迪茨氏菌(Dietzia sp.)和拉布伦茨氏菌(Labrenzia sp.);经优化,SQ1在最适条件下(30℃、p H7.6、石油浓度20 g/L),11 d内对石油的去除率高达73.5%;GC-MS结果表明,复合菌系SQ1对石油总烷烃的去除率为91.7%,对较难降解的C21-C35烷烃组分的降解率接近100%。研究表明,复合菌系SQ1在海洋石油污染的生物修复中具有较强的应用潜力。 | 吴秉奇 刘淑杰 陈福明 周楚莹 | 2016 | 生物技术通报2016，32，8： | 10 |
| 3 | 废弃矿井微生物群落演替特征实验研究显示文摘矿井被关闭前后,井下环境条件有很大的差异,尤其是温度、压力、光照、水质、氧化还原条件会发生明显的改变,由此引起废弃矿井中微生物群落结构、多样性、演替规律发生变化,微生物的演替也将影响到井下污染物的降解和矿井水水质。该研究利用井下原煤、煤泥、矿井水进行了废弃矿井封闭和半封闭状态下的模拟实验,探讨了2种模拟中的微生物群落演替及多样性变化特征,实验周期为135 d。实验结果表明,矿井关闭后,细菌为环境中可培养好氧微生物的绝对优势菌群,占比例达到90%以上。封闭和半封闭条件下细菌丰度和多样性均呈增加趋势,但受环境中氧含量的影响,封闭条件下厌氧微生物丰度和多样性出现明显的增加。利用高通量测序技术,分析了细菌群落的演替规律,结果表明,封闭及半封闭条件下实验体系中优势细菌主要为芽孢杆菌(Bacillus)和乳球菌(Lactococcus)等兼性厌氧菌,随着时间变化,亚硝化单胞菌(Nitrosomonadaceae)、硝化螺菌(Nitrospira)和节杆菌(Arthrobacter)等好氧菌逐渐衰亡,乳酸杆菌(Lactobacillus)、拟杆菌(Bacteroides)、链球菌(Streptococcus)、明串珠菌(Leuconostoc)等厌氧菌含量逐渐增加。 | 单爱琴 张燕婷 肖洁 冯启言 高波 | 2019 | 环境科学与技术2019，42，4： | 4 |
| 4 | 炼油废水中筛选的一株高效降解石油烃菌株的鉴定及降解特性显示文摘近年来,石油污染造成的环境问题日益严重,寻找高效的石油降解菌株成为石油污染微生物修复的关键因素。通过对炼油废水中筛选出的一株高效降解石油烃菌株L4进行菌株形态特征观察、生理生化特性实验以及16SrDNA序列分析,鉴定其为苍白杆菌(Ochrobactrum ciceri)。并对L4降解石油烃效果、降解动力学及降解过程中的菌体浓度变化进行了研究,结果表明:在初始质量浓度为4 000mg/L的石油烃中加入该菌株,120r/min、30℃下振荡培养6d后,石油烃降解率高达98.98%。L4降解石油烃的动力学曲线与零级动力学方程拟合效果良好,半衰期为2.3d。L4在降解石油烃的0~1d,菌体生长较为迟缓,随后进入对数生长期;4d后,生长速率逐渐降低直至为零,石油烃降解率也缓慢增加,直至趋于稳定。 | 高鹏飞 刘虹 刘翠珠 温钢 | 2018 | 环境污染与防治2018，40，12： | 4 |
| 5 | Exploring the Degradation Potential of Halomonas Bacteria from Oil-contaminated Marine Environment显示文摘The degradation potential of a diesel-degrading bacteria(HDMP1) isolated from the oil-contaminated marine environment was explored systematically by analyzing the environmental conditions and synergistic action with other dieseldegrading bacteria. The result indicated that HDMP1 was confirmed as a strain of Halomonas by the method of strain identification. And, HDMP1 showed good diesel degradation performance with a diesel degradation rate of up to 79.59%after 7 days. By analyzing the effect of environmental conditions on HDMP1, the best carbon and nitrogen sources were found to be lactose and peptone, respectively, at a pH value of 7.5 and a salinity of 4 g/(100 mL). Additionally, the synergistic effect of HDMP1 combined with other diesel-degrading bacteria was analyzed by orthogonal experimental design. The inocula of HDMP1, HDMP2, HDMP3 and HDMB3 were optimized, with the best results equating to 0.4%, 0.1%, 0.4% and0.9% in 100 mL of MSM, respectively, while the degradation rate of diesel was identified to be 73.5% within 5 days in the presence of optimum inocula. | Wu Yanan Liu Yingying Xue Jianliang Shi Ke Gao Yu Xiao Xiaolong | 2018 | China Petroleum Processing & Petrochemical Technology2018，20，4： | 2 |
| 6 | Preparation and Lubricating Properties of A New Antibacterial Emulsion Containing Nano-TiO_2 for Cold Rolling Strips显示文摘A new kind of emulsion containing nano TiO_2 was developed through the dispersion experiment. A commercial emulsion and a prepared by our lab emulsion without nano particles were chosen as controls to test the tribological and antibacterial properties of this new emulsion. The load carrying capacity, friction coefficient and average diameter of wear scars were tested by a four-ball machine and the comprehensive antifriction parameter ω was calculated. The wetting angle was also tested using a JC200C1 wetting angle tester. The micro surface and roughness of rolled strips were analyzed to investigate the tribological performance of the recommended new emulsion in strip production. It is shown that the new nano-emulsion possesses a higher load carrying capacity and wetting ability. Therefore the abrasive/plowing wear is reduced more efficiently with the addition of nano particles, and the micro surface is improved. The density of bacteria in the emulsions was tested after the cold rolling experiment. The emulsion breaking ratio and bacteria density were also tested in different time intervals after the cold rolling experiment. The final p H values and bacteria density of different layers of emulsions were measured and the sediment was analyzed by TEM to evaluate the antibacterial behavior of this new emulsion. It is shown that the density of microbial colonies which led to a corruption of emulsions was decreased about 90% and the effective antibacterial period was prolonged. | Lu Yudi Sun Jianlin Zhang Bingtao | 2016 | China Petroleum Processing & Petrochemical Technology2016，18，3： | 2 |
| 7 | 不同微生物组合对石油烷烃降解性能的比较分析显示文摘以大连石油污染海域的海洋沉积物作为分离样品,以天然海水培养基分离获得13种海洋石油降解微生物,并通过模拟海底低温微氧环境,以原油作为唯一碳源,评价了13种微生物的石油降解性能。GC-MS分析结果表明,随着降解时间的增加,组合G6及其构建菌株P73和P83对烷烃的降解率逐渐升高。对于C>32的长链烷烃部分,尤其对于石油中的难降解组分如姥鲛烷(Pr)和植烷(Ph)的降解,组合G6的降解效果明显优于单菌株。由高效石油降解菌菌株P73和菌株P83构建的组合G6对于石油烷烃中很难降解和风化的长链烷烃具有强大的协同降解作用。 | 陈帅 刘秋 富洋 袁烽皓 张宛星 赵乃琦 | 2019 | 大连民族大学学报2019，21，3： | 0 |
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