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| 1 | The MADS transcription factor CmANR1 positively modulates root system development by directly regulating CmPIN2 in chrysanthemum显示文摘Plant root systems are essential for many physiological processes,including water and nutrient absorption.MADS-box transcription factor(TF)genes have been characterized as the important regulators of root development in plants;however,the underlying mechanism is largely unknown,including chrysanthemum.Here,it was found that the overexpression of CmANR1,a chrysanthemum MADS-box TF gene,promoted both adventitious root(AR)and lateral root(LR)development in chrysanthemum.Whole transcriptome sequencing analysis revealed a series of differentially expressed unigenes(DEGs)in the roots of CmANR1-transgenic chrysanthemum plants compared to wild-type plants.Functional annotation of these DEGs by alignment with Gene Ontology(GO)terms and biochemical pathway Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis indicated that CmANR1 TF exhibited“DNA binding”and“catalytic”activity,as well as participated in“phytohormone signal transduction”.Both chromatin immunoprecipitation–polymerase chain reaction(ChIP-PCR)and gel electrophoresis mobility shift assays(EMSA)indicated the direct binding of CmPIN2 to the recognition site CArG-box motif by CmANR1.Finally,a firefly luciferase imaging assay demonstrated the transcriptional activation of CmPIN2 by CmANR1 in vivo.Overall,our results provide novel insights into the mechanisms of MADS-box TF CmANR1 modulation of both AR and LR development,which occurs by directly regulating auxin transport gene CmPIN2 in chrysanthemum. | Cui-Hui Sun Jian-Qiang Yu Xi Duan Jia-Hui Wang Quan-Yan Zhang Kai-Di Gu Da-Gang Hu Cheng-Shu Zheng | 2018 | Horticulture Research2018，5，1： | 1 |
| 2 | 番茄SlCBL1基因的克隆与生物学功能初探显示文摘类钙调磷酸酶B亚基蛋白(calcineurin B-like proteins,CBLs)是植物中一类特殊的钙离子感受器,在应对多种生物与非生物胁迫反应中起到十分重要的作用.目前有关CBLs基因生物学功能研究主要集中于拟南芥与水稻,番茄中相关功能报道较少.研究利用同源克隆方法,从番茄中分离出1个SlCBL1基因;应用半定量PCR技术研究SlCBL1基因在不同组织的相对表达水平,将该基因构建超表达载体并遗传转化本氏烟.结果表明:SlCBL1基因cDNA全长639 bp,编码212个氨基酸残基;基因组全长5000 bp,包含8个外显子及7个内含子;推导的氨基酸序列与烟草NtCBL1蛋白相似度最高,且包含CBL蛋白结合Ca2+所必需的4个EF手型结构.SlCBL1在番茄不同组织中均有表达,但在营养器官中的表达最高,并且响应多种非生物胁迫.与对照相比,过超量表达SlCBL1基因的本氏烟株系提高了对盐胁迫的抗性.上述研究表明,番茄SlCBL1基因能够响应并拮抗非生物胁迫,这为进一步研究CBL1生物学功能提供了基础. | 刘敏 张云 闵康康 陈丽双 杨玲 王长春 | 2020 | 浙江师范大学学报(自然科学版)2020，43，4： | 0 |
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