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| 1 | A Domestication-Associated Gene GmPRR3b Regulates the Circadian Clock and Flowering Time in Soybean显示文摘Improved soybean cultivars have been adapted to grow at a wide range of latitudes,enabling expansion of cultivation worldwide.However,the genetic basis of this broad adaptation is still not clear.Here,we report the identification of GmPRR3b as a major flowering time regulatory gene that has been selected during domestication and genetic improvement for geographic expansion.Through a genome-wide association study of a diverse soybean landrace panel consisting of 279 accessions,we identified 16 candidate quantitative loci associated with flowering time and maturity time.The strongest signal resides in the known flowering gene E2,verifying the effectiveness of our approach.We detected strong signals associated with both flowering and maturity time in a genomic region containing GmPRR3b.Haplotype analysis revealed that GmPRR3bH6 is the major form of GmPRR3b that has been utilized during recent breeding of modern cultivars.mRNA profiling analysis showed that GmPRR3bH6 displays rhythmic and photoperiod-dependent expression and is preferentially induced under long-day conditions.Overexpression of GmPRR3bH6 increased main stem node number and yield,while knockout of GmPRR3bH6 using CRISPR/Cas9 technology delayed growth and the floral transition.GmPRR3bH6 appears to act as a transcriptional repressor of multiple predicted circadian clock genes,including GmCCAIa,which directly upregulates J/GmELF3a to modulate flowering time.The causal SNP(Chr12:5520945)likely endows GmPRR3bH6 a moderate but appropriate level of activity,leading to early flowering and vigorous growth traits preferentially selected during broad adaptation of landraces and improvement of cultivars. | Cong Li Ying-hui Li Yanfei Li Hongfeng Lu Huilong Hong Yu Tian Hongyu Li Tao Zhao Xiaowei Zhou Jun Liu Xinan Zhou Scott A.Jackson Bin Liu Li-juan Qiu | 2020 | Molecular Plant2020，13，5： | 11 |
| 2 | 中国转基因植物产业化的研究进展及存在问题显示文摘综合分析了全球与中国转基因植物的产业化现状,结果表明中国转基因植物产业化已取得重大成果,在某些作物上已达到国际先进水平,转基因作物的种植面积居全球第5位。但总体看尚处于发展阶段,与发达国家相比还有一定差距。本文分析了中国转基因植物研究中存在的不足及其产业化进程滞后的原因,并对中国今后的转基因植物产业化发展提出了建议。 | 王关林 孙月剑 那杰 李洪艳 方宏筠 | 2006 | 中国农业科学2006，39，7： | 10 |
| 3 | 骆驼刺发根农杆菌转化系的原生质体培养和植株再生显示文摘从发根农杆菌A4转化的荒漠植物——骆驼刺毛状根愈伤组织中分离的原生质体培养的结果表明,酶解新转代7~10d的淡黄色松软愈伤组织,可获得大量有活力的原生质体。原生质体在附加有1.5mg·L-12,4-D、0.2mg·L-16-BA、0.3mol·L-1甘露醇、2%(W/V)蔗糖和500mg·L-1水解酪蛋白的DPD培养基中进行液体浅层培养可持续分裂。培养基的最适渗透压为(450±3)mOsm·kg-1,原生质体的最适植板密度为4×105个·mL-1。制备原生质体的愈伤组织以低温(4℃)预处理后,原生质体的产率和分裂频率均提高,分裂频率最高可达50%。原生质体分裂形成的愈伤组织转移在附加1~2mg·L-16-BA(或KT)和0.2mg·L-1NAA的MS培养基上培养后,可以分化并获得再生植株。纸电泳检测表明,原生质体再生的愈伤组织和分化植株仍然含有毛状根转化系的特异产物——冠瘿碱。 | 张改娜 贾敬芬 | 2009 | 植物生理学通讯2009，45，4： | 4 |
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