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| 1 | 烟草NtD14基因的遗传转化及其功能显示文摘D14是独脚金内酯信号传导关键基因。为了研究烟草D14基因(Nt D14)的功能,笔者利用同源克隆的方法克隆Nt D14基因,然后构建p CAMBIA1302-Nt D14植物过表达载体,通过农杆菌介导的叶盘转化法将其转化烟草。结果表明,过表达Nt D14基因烟草的株高低于野生型,叶片变短变小,但叶片长宽比无显著差异,转化株分枝数减少。ELISA分析发现,所有过表达Nt D14转基因株系生长素含量低于野生型,说明Nt D14基因转化烟草株高和叶片变小可能与生长素含量减低有关。此外,转基因株系的丙二醛(MDA)含量和过氧化氢酶(CAT)活性均显著高于野生型。转基因株系的过氧化物酶(POD)活性显著低于野生型,CAT和POD的含量成反比,但超氧化物歧化酶(SOD)的含量无显著性差异,说明过表达Nt D14基因影响烟草体内的氧化还原反应。 | 徐琳 张泽 吕贤哲 李肇通 黄惜 | 2018 | 热带生物学报2018，9，4： | 2 |
| 2 | 水稻分蘖与植株碳氮化合物含量和分蘖相关基因表达量关系分析显示文摘以分蘖特性不同的4个寒地粳稻品种和2个籼稻品种为供试材料,通过盆栽试验研究水稻分蘖发生与植株可溶性糖、全氮含量及分蘖相关基因转录表达量关系。结果表明,分蘖发生起始时间、速度及最高茎数等分蘖特性主要受遗传因素控制,穗数型品种分蘖早生快发;在分蘖过程中高分蘖品种植株可溶性糖含量和糖氮比均显著高于低分蘖品种,全氮含量与此相反;分蘖过程中高分蘖品种OsTB1、OsD3和OsD14基因转录表达量均显著低于低分蘖品种,OsMADS57基因转录表达量均显著高于低分蘖品种;分蘖发生数与分蘖不同时期植株全氮含量呈不显著负相关,与可溶性糖含量、糖氮比及OsMADS57基因转录表达量呈显著正相关,与OsTB1、OsD3、OsD14基因转录表达量呈显著负相关。 | 金正勋 张玉磊 潘冬 同拉嘎 李丹 李明月 王海微 韩云飞 张忠臣 | 2017 | 东北农业大学学报2017，48，7： | 1 |
| 3 | Dissecting the genetic basis of maize deep-sowing tolerance by combining association mapping and gene expression analysis显示文摘Deep-sowing is an important method for avoiding drought stress in crop species,including maize.Identifying candidate genes is the groundwork for investigating the molecular mechanism underlying maize deep-sowing tolerance.This study evaluated four traits(mesocotyl length at 10 and 20 cm planting depths and seedling emergence rate on days 6 and 12)related to deep-sowing tolerance using a large maize population containing 386 inbred lines genotyped with 0.5 million high-quality single nucleotide polymorphisms(SNPs).The genomewide association study detected that 273 SNPs were in linkage disequilibrium(LD)with the genetic basis of maize deep-sowing tolerance.The RNA-sequencing analysis identified 1944 and 2098 differentially expressed genes(DEGs)in two comparisons,which shared 281 DEGs.By comparing the genomic locations of the 273 SNPs with those of the 281 DEGs,we identified seven candidate genes,of which GRMZM2G119769 encoded a sucrose non-fermenting 1 kinase interactor-like protein.GRMZM2G119769 was selected as the candidate gene because its homologs in other plants were related to organ length,auxin,or light response.Candidate gene association mapping revealed that natural variations in GRMZM2G119769 were related to phenotypic variations in maize mesocotyl length.Gene expression of GRMZM2G119769 was higher in deep-sowing tolerant inbred lines.These results suggest that GRMZM2G119769 is the most likely candidate gene.This study provides information on the deep-sowing tolerance of maize germplasms and identifies candidate genes,which would be useful for further research on maize deep-sowing tolerance. | YANG Yue MA Yu-ting LIU Yang-yang Demar LYLE LI Dong-dong WANG Ping-xi XU Jia-liang ZHEN Si-han LU Jia-wen PENG Yun-ling CUI Yu FU Jun-jie DU Wan-li ZHANG Hong-wei WANG Jian-hua | 2022 | Journal of Integrative Agriculture2022，21，5： | 0 |
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