| 1 | 急性脊髓损伤动物模型的建立与评估显示文摘背景:目前国内外脊髓损伤模型的设计种类繁多,各有其优缺点,但尚未有一种客观化、标准化的模型以满足临床研究。目的:设计一种简易打击器并建立脊髓损伤模型,通过与NYU(New York University)打击器建立的脊髓损伤模型进行对比,评估自制改良Allen’s脊髓损伤模型的参数及稳定性。方法:雌性SD大鼠随机分为5组,即假手术组(A组6只),自制打击器5,10 cm组(B1,B2组),NYU打击器1.25,2.5 cm组(C1,C2组),A组不打击,其余各组根据分组的不同高度进行打击,每组6只;各组分别与造模术后1,3,5,7 d进行行为学评分,冰冻切片后用尼氏染色法观察病理学改变,并进行半定量分析。结果与结论:(1)在行为学评分、脊髓损伤面积比和脊髓前、后角神经元半定量分析中,同一时间点,其他4组与A组比较差异均有显著性意义(P<0.05),B1组和C1组、B2组和C2组差异无显著性意义(P>0.05),B1组和B2组、C1组和C2组差异均有显著性意义(P<0.05);(2)病理学改变:A组见脊髓灰、白质分界清晰,呈蝴蝶状,神经元细胞核大、核仁明显、数量较多,胞质内斑块状或虎斑样尼氏体清晰;余各组出现不同程度的淤血灶,神经元数目减少,损伤处可见细胞肿胀,轴突脱髓鞘改变,部分细胞呈空泡样改变,尼氏体模糊或消失;(3)结果证实,自制打击器能制备出不同损伤程度的脊髓损伤模型,与NYU脊髓损伤模型效果相近,可靠性高,稳定性好,且操作简单,易于推广,可为大鼠脊髓损伤的研究提供基础。 | 吴杨鹏 范筱 张俐 | 2016 | 中国组织工程研究2016，20，49： | 27 |
| 2 | Tamoxifen and Src kinase inhibitors as neuroprotective/neuroregenerative drugs after spinal cord injury显示文摘Spinal cord injury(SCI) is a devastating condition that produces significant changes in the lifestyle of patients. Many molecular and cellular events are triggered after the initial physical impact to the cord. Two major phases have been described in the field of SCI: an acute phase and late phase. Most of the therapeutic strategies are focused on the late phase because this provides an opportunity to target cellular events like apoptosis, demyelination, scar formation and axonal outgrowth. In this mini-review, we will focus on two agents(tamoxifen and a Src kinase family inhibitor known as PP2) that have been shown in our laboratory to produce neuroprotective(increase cell survival) and/or regenerative(axonal outgrowth) actions. The animal model used in our laboratory is adult female rat(~250 g) with a moderate contusion(12.5 mm) to the spinal cord at the T10 level, using the MASCIS impactor device. Tamoxifen or PP2 was administered by implantation of a 15 mg pellet(Innovative Research of America, Sarasota, FL, USA) or by intraperitoneal injections(1.5 mg/kg, every 3 days), respectively, to produce a long-term effect(28 days). Tamoxifen and the Src kinase inhibitor, PP2, are drugs that in rats with a moderate spinal cord injury promote functional locomotor recovery, increase spared white matter tissue, and stimulate axonal outgrowth. Moreover, tamoxifen reduces the formation of reactive oxygen species. Therefore, these drugs are possible therapeutic agents that have a neuroprotective/regenerative activity in vertebrates with SCI. | Iris K.Salgado Aranza I.Torrado Jose M.Santiago Jorge D.Miranda | 2015 | Neural Regeneration Research2015，10，3： | 5 |
| 3 | 增液汤联合开塞露治疗脊髓损伤后长期便秘患者的疗效观察显示文摘目的探讨增液汤联合开塞露治疗脊髓损伤后长期便秘患者的临床疗效。方法选取2014年3月至2016年4月于广东省工伤康复医院就诊治疗的60例脊髓损伤后长期便秘患者作为研究对象,依据数字表法将上述患者随机分为两组,对照组患者给予常规治疗配合开塞露,观察组患者在对照组治疗基础上联合使用增液汤进行治疗。观察并比较两组患者的治疗效果、舒适率及生命质量评分。结果观察组患者经治疗后临床治疗总有效率达96.7%,相较于对照组的80.0%明显提高,差异有统计学意义(P<0.05);另外比较两组舒适率及生命质量评分,观察组较对照组明显提高,差异均有统计学意义(均P<0.05)。结论采用增液汤联合开塞露治疗脊髓损伤后长期便秘可有效提高该病症的临床治疗效果,提高患者诊疗舒适度,减轻痛苦,改善其生命质量。 | 王秀丽 钟伟湘 张海平 李尚 梁碧彦 | 2017 | 中国药物经济学2017，12，3： | 1 |
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