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| 1 | 异枝江蓠切段培养的初步研究显示文摘文章分别从不同部位、不同的消毒处理方法、不同的切段方法、以及不同的切段长度4个方面对异枝江蓠(Gracilariabailinae)进行了组织切段培养的研究。研究结果表明,在不同部位的切段实验中,发现主干部位出芽数量显著多于分枝中部与茎尖的出芽数量,为最适合异枝江蓠切段培养的取材部位。在不同消毒方法的实验中,综合成活率与杂藻污染率来看,先经0.5g/L青霉素钠+0.5g/L硫酸链霉素+1mg/L GeO2处理16h,然后用消毒海水培养48h的预处理,再用70%的酒精处理5s或1.5%KI处理10min消毒效果较好。在不同切段方法的实验中,斜切与横切的出芽数差异不显著。在不同切段长度的实验中,长度为2mm的切段芽密度最大,为最经济的切段长度。大多数切段的下切面均未发现有新芽长出,表现出明显的极性。 | 陈海红 陈伟洲 | 2017 | 海洋湖沼通报2017，，6： | 1 |
| 2 | Isolation and callus formation of Gracilariopsis bailiniae(Gracilariales, Rhodophyta) protoplasts显示文摘This paper reports the first successful isolation of protoplasts from G racilariopsis bailiniae and their callus formation. The base solution type, concentration of isolating enzymes, concentration of sorbitol, incubation time, temperature and pH of the enzyme solution were tested to optimize the protoplast yield. The optimized isolation conditions were: 40% base solution 3(deionized water containing 25 mmol/L MESTris and 25 mmol/L CaCl 2 ·2 H 2 O) and 60% crude Marinomonas sp. YS-70 agarase solution, containing 2% w/v cellulase, 1% w/v macerozyme R-10 and 0.4 mol/L sorbitol, with incubation for 4 h at 28°C and pH 6.5. The highest yield of viable protoplasts, which was obtained in these conditions, was(1.75±0.25)×10 6 cells/g fresh weight. Cell wall regeneration of most protoplasts from G. bailiniae was complete within 60 h and the first division of cells happened after ≥3 days. Two division types were observed in the first division of protoplasts from G. bailiniae— asymmetric division and symmetric division. After the first division, the cells underwent a series of divisions to form callus cell masses. | 陈海红 陈伟洲 石经仪 陈泽攀 张毅 | 2018 | Journal of Oceanology and Limnology2018，36，6： | 0 |
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