共被期刊论文引用了2次
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| 1 | 神经生长因子及其在眼科领域的应用显示文摘神经生长因子(nerve growth factor,NGF)是神经经营养因子的一种。广泛存在于神经组织和周围靶组织,在神经系统的发生、发育和损伤修复过程中起着重要作用,可促进神经细胞的存活增殖、分化及功能表达。本文就NGF的生物学特性、眼组织中表达及对眼病的影响进行综述。 | 朱丹丹 陈辉 | 2007 | 中国实用眼科杂志2007，25，9： | 2 |
| 2 | A novel xeno-free culture system for human retinal pigment epithelium cells显示文摘AIM: To find out an animal-free, xeno-free culture method for human fetal retinal pigment epithelium(fRPE) cells aiming for cell-replacement therapy. METHODS: Human AB serum, knock-out serum replacement(KSR) and B27 were supplemented as a substitute of fetal bovine serum(FBS) in culture medium of human fRPE cells. Cell morphology was examined by light microscope and transmission electron microscope. Proliferation ability was detected by cell cycle analysis and examination of KI67 expression. Apoptosis was analyzed using FACS. The expression ofRPE-specific markers was demonstrated by quantitative real-time polymerase chain reaction(qPCR), Western blot(WB) and immunocytochemistry. Paracrine function was determined using enzyme-linked immunosorbent assay method.RESULTS: Our results indicated that the optimum concentration of KSR was 15%, the optimum concentration of B27 was 2%, and the optimum concentration of human AB serum was 10%. fRPE cells cultured in 15% KSR and 2% B27 media showed repressed propagation and differentiation ability, and gradually lost epithelial morphology and RPE function. While fRPE cells cultured in 10% human AB serum media showed a typical cobblestone morphology with pigmentation, elevated proliferation ability, satisfying paracrine function and expressed RPE-specific markers. CONCLUSION: Our study indicates that culturing fRPE cells in 10% human AB serum-supplemented medium is more favorable compared with KSR, B27 and traditional FBS-supplemented mediums when fRPE cells are to be applied in cell-based therapy. | Han Shen Min Wang Duo Li Song-Tao Yuan Qing-Huai Liu | 2019 | International Journal of Ophthalmology(English edition)2019，12，4： | 1 |
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