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| 1 | Apoptosis of hepatoma cells SMMC-7721 induced by Ginkgo biloba seed polysaccharide显示文摘AIM: To study the apoptosis of hepatoma cells SMMC-7721induced by polysaccharide isolated from Ginkgo biloba seed.METHODS: Ginkgo biloba seed polysaccharide (GBSP) wasisolated by ethanol fractionation of Ginkgo biloba seed andpurified by Sephadex G-200 chromatography. The purity ofGBSP was verified by reaction with iodine-potassium iodideand ninhydrin and confirmed by UV spectrophotometer,cellulose acetate membrane electrophoresis and Sepharose4B gel filtration chromatography. The Scanning ElectronMicroscope (SEM) and Flow Cytometrv (FCM) were used toexamine the SMMC-7721 cells with and without GBSPtreatment at 500 mg/ml for 36 h.RESULTS: GBSP product obtained was of high purity withthe average molecular weight of 1.86 × 105. Quantitativeanalysis of SMMC-7721 cells in vitro with FCM showed thatthe percentages of G2-M cells without and with GBSPtreatment were 17.01±1.28 % and 11.77±1.50% (P<0.05),the debds ratio of the cells were 0.46±0.12 % and 0.06±0 .06 %(P<0.01), and the apoptosis ratio of cells was 3.84±0 .55 %and 9.13±1.48 %(P<0.01) respectively. Following GBSPtreatment, microvilli of SMMC-7721 cells appeared thinnerand the number of spherical cells increased markedly. Mostsignificantly, the apoptosis bodies were formed on andaround the spherical cells treated with GBSP.CONCLUSION: GBSP could potentially induce the apoptosisof SMMC-7721 cells. | Gui-Wen Yang Li-Guo An Department of Biology,Shandong Normal University,Jinan 250014,Shandong Province,China Qun Chen Department of Biology & Chemistry,Huainan Teachers College,Huainan 232001,Anhui Province,China | 2002 | World Journal of Gastroenterology2002，8，5： | 15 |
| 2 | 微波外科技术行兔脾部分切除术后病理及超微结构观察显示文摘目的 探讨微波外科技术在脾部分切除术中的应用价值。方法 微波组织凝固 (MTC)行兔脾部分切除术 0h、术后 7d和 1 4d分别切取脾脏行病理与电镜检查。结果 光镜下脾组织凝固止血带呈坏死区、致密区和边缘区三个区域。术后 1 4d,纤维隔在组织内形成 ,透射电镜显示脾的网状细胞接近正常 ,细胞线粒体嵴尚不完整。结论 MTC行脾部分切除对脾的损害短期内可修复。 | 鞠新华 夏振龙 魏林 张激杨 王之章 李承彬 刘冬娟 | 2003 | 中华物理医学与康复杂志2003，25，6： | 2 |
| 3 | Fos expression in tyrosine hydroxylase-containing neurons in rat brainstem after visceral noxious stimulation:an immunohistochemical study显示文摘AIM: To prove that neurons in the different structures of the brainstem that express tyrosine hydroxylase (TH) are involved in the transmission and modulation of visceral or somatic nociceptive information in rat.METHODS: Immunohistochemical double-staining method was used to co-localize TH and Fos expression in neurons of the rat brainstem in visceral or subcutaneous noxious stimulation models.RESULTS: Neurons co-expressing TH/Fos were observed in lateral reticular nucleus (LRT), rostroventrolateral reticular nucleus (RVL), solitary tract nucleus (SOL), locus coeruleus (LC), A5, A7 neuronal groups and ventrolateral subdivision of the periaqueductal gray (vlPAG) in both models. But the proportion and number of the double-labeled neurons responding to the two noxious stimuli were significantly different in the LRT, RVL and LC nuclei. The proportion and number of the TH/Fos double-labeled neurons in the visceral pain model were smaller than that in the subcutaneous pain model. However, in the case of SOL, they were similar in the two models.CONCLUSION: Differences of Fos expression in TH immunoreactive neurons in animals after visceral and somatic noxious stimulation indicate that the mechanisms of the transmission and modulation of visceral nociceptive information in the brainstem may be different from that of somatic nociceptive information. | Feng Han Yu-Fei Zhang Yun-Qing Li Department of Anatomy and K.K.Leung Brain Research Centre,the Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2003 | World Journal of Gastroenterology2003，9，5： | 2 |
| 4 | 烧创伤后免疫功能障碍识别研究进展显示文摘脓毒症是烧(创)伤后的严重并发症之一。欧洲一组临床流行病学资料显示,2527例全身炎性反应患者中,脓毒症发生率为26.0%,病死率16.0%。 | 于燕 杨红明 姚咏明 | 2007 | 中华烧伤杂志2007，23，6： | 1 |
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