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| 1 | 磁性固相萃取技术在食品真菌毒素分析中的应用显示文摘真菌毒素常见于食品和各种粮食作物中,具有致癌性、诱变性、致畸性等危害,严重威胁人类和动物的健康。由于真菌毒素在食品基质中存在种类多、浓度低、极性范围广等特点,因此建立快速高效的食品样品前处理方法对真菌毒素的痕量分析十分重要。磁性固相萃取(magnetic solid-phase extraction,MSPE)是一种基于磁相互作用的样品前处理技术,凭借简单快速、绿色安全、高效经济等特点,已广泛应用于食品样品真菌毒素分析的前处理中。本文介绍了MSPE的萃取过程和磁性纳米材料的制备和修饰,综述了以无机材料(氧化硅基和碳基)、有机材料(印迹型、有机高分子、表面活性剂和有机小分子)和其他材料(离子液体和单克隆抗体)为代表的MSPE吸附剂在食品样品真菌毒素检测中的应用,并对MSPE技术的发展趋势进行了展望。 | 徐洪文 徐华 朱瑜 叶永丽 孙嘉笛 孙秀兰 张晓娟 | 2021 | 食品科学2021，42，11： | 4 |
| 2 | Analytical methods for locating modifications in nucleic acids显示文摘In addition to the canonical nucleobases, a variety of chemical modifications have been identified presence in nucleic acids. These modifications have been demonstrated to involve in regulating the spatiotemporal expression of genes. Up to date, over 150 types of chemical modifications have been found existence in nucleic acids. Understanding the functional roles of modifications relies on deciphering the location information of modifications in nucleic acids. Analytical methods for studying nucleic acid modifications have greatly advanced in the last decade. To locate the modifications in nucleic acids, various mass spectrometry (MS)-based analytical strategies have been established. Recent progress in next-generation sequencing (NGS) in conjugation with immunoprecipitation, chemical reaction, enzyme-mediated mutation, or nanomaterials offer genome-wide or transcriptome-wide mapping of modifications, which greatly revolutionize the field of epigenetic modifications. Herein, we reviewed and summarized the established methods and the breakthrough of the techniques for locating modifications in nucleic acids. In addition, we discussed the principles, applications, advantages and drawbacks of these methods. We believe that with the rapid advancement of techniques and methods,the functions of nucleic acid modifications will be fully understood in the future. | Chubo Qi Jianghui Ding Bifeng Yuan Yuqi Feng | 2019 | Chinese Chemical Letters2019，30，9： | 2 |
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