|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 白藜芦醇抗氧化功能的研究进展显示文摘白藜芦醇是一种主要存在于葡萄中的多酚化合物。据报道,白藜芦醇具有抗氧化,延长生命以及能量代谢调节作用,同时白藜芦醇还对应激和携带疾病的啮齿类动物具有保护作用。文章主要综述了白藜芦醇抗氧化功能及其作用的相关分子机制,旨在为白藜芦醇在畜禽生产中的合理应用提供科学理论依据。 | 潘鹏 谢红月 蒋钦杨 黄艳娜 | 2020 | 饲料工业2020，41，2： | 9 |
| 2 | lncRNA MEG3在2型糖尿病伴Hp感染患者血清中的表达及意义显示文摘目的探讨长链非编码RNA MEG3(lncRNA MEG3)在2型糖尿病(T2DM)伴幽门螺杆菌(Hp)感染患者血清中的表达及意义。方法选取132例T2DM患者为研究对象,根据是否感染Hp分为非感染Hp组(49例)和感染Hp组(83例)。实时荧光定量PCR(RT-qPCR)检测两组患者血清lncRNA MEG3水平,全自动分析仪检测血清游离脂肪酸(FFA)水平,乳胶增强免疫(超敏)比浊法检测超敏C反应蛋白(hs-CRP)、酶联免疫吸附法检测肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)、丙二醛(MDA)水平、超氧化物歧化酶(SOD)和谷胱甘肽还原酶(GSH-Px)表达水平。比较两组患者各检测指标水平差异,并进行Pearson相关性分析。Logistic回归分析影响T2DM患者感染Hp的因素。结果与非感染Hp组比较,感染Hp组患者血清lncRNA MEG3、SOD和GSH-Px水平显著降低(P<0.05),FFA、TNF-α、IL-6、hs-CRP和MDA水平均显著升高(P<0.05)。Pearson相关性分析结果显示,T2DM伴Hp感染患者血清lncRNA MEG3水平与FFA、TNF-α、IL-6、hs-CRP和MDA呈负相关(P<0.05),与SOD和GSH-Px呈正相关(P<0.05)。Logistic多因素分析结果显示,lncRNA MEG3是影响T2DM患者感染Hp的独立因素(P<0.05)。结论lncRNA MEG3在T2DM伴Hp感染患者血清中表达降低,其可能通过调节机体内脂肪酸水平、炎性因子表达及氧化应激水平参与T2DM感染Hp过程。 | 田艳娟 杨小东 刘成功 | 2021 | 广东医学2021，42，2： | 6 |
| 3 | 基于mTOR通路探讨中医药调控心肌缺血/再灌注损伤中自噬的研究进展显示文摘自噬是广泛存在于真核细胞中用以维持细胞稳态的一种降解途径,在多种心血管疾病尤其是心肌缺血/再灌注损伤的发生、发展中起着重要的调控作用,是治疗心血管疾病的新靶点。哺乳动物雷帕霉素靶蛋白(mTOR)通路是自噬调控的诸多信号通路中的下游,在心血管疾病自噬过程中起重要作用,可调控心脏自噬,促进自噬流以改善心脏功能。中医药治疗心肌缺血/再灌注损伤的作用机制与自噬密切相关,且具有双向调节自噬的优势。文章拟围绕mTOR通路,对当前可通过调控自噬以保护心肌缺血/再灌注损伤的中药成分及复方研究进展作一综述,以期为未来相关中医药干预自噬研究提供思路。 | 李檫 李益萍 王肖龙 | 2021 | 中华中医药杂志2021，36，12： | 5 |
| 4 | Effects of dietary tributyrin on intestinal mucosa development,mitochondrial function and AMPK-mTOR pathway in weaned pigs显示文摘Background:The objective of this experiment was to investigate the influence of dietary tributyrin on intestinal mucosa development,oxidative stress,mitochondrial function and AMPK-mTOR signaling pathway.Methods:Seventy-two pigs were divided into two treatments and received either a basal diet or the same diet supplemented with 750 mg/kg tributyrin.Each treatment has six replicates of six pigs.After 14 days,6 pigs from each treatment were selected and the jejunal samples were collected.Results:Results showed that supplemental tributyrin increased(P<0.05)villus height and villus height:crypt depth of weaned pigs.Pigs fed tributyrin had greater(P<0.05)RNA/DNA and protein/DNA ratios than pigs on the control group.The mRNA levels of sodium glucose transport protein-1 and glucose transporter-2 in the jejunum were upregulated(P<0.05)in pigs fed the tributyrin diet.Dietary tributyrin supplementation lowered(P<0.05)the malondialdehyde and hydrogen peroxide(H2O2)content in jejunum,enhanced(P<0.05)the mitochondrial function,as demonstrated by decreased(P<0.05)reactive oxygen species level and increased(P<0.05)mitochondrial membrane potential.Furthermore,tributyrin increased(P<0.05)mitochondrial DNA content and the mRNA abundance of genes related to mitochondrial functions,including peroxisomal proliferator-activated receptor-γcoactivator-1α,mitochondrial transcription factor A,nuclear respiratory factor-1 in the jejunum.Supplementation with tributyrin elevated(P<0.05)the phosphorylation level of AMPK and inhibited(P<0.05)the phosphorylation level of mTOR in jejunum compared with the control group.Conclusions:These findings suggest that dietary supplementation with tributyrin promotes intestinal mucosa growth,extenuates oxidative stress,improves mitochondrial function and modulates the AMPK-mTOR signal pathway of weaned pigs. | Chunchun Wang Shuting Cao Zhuojun Shen Qihua Hong Jie Feng Yan Peng Caihong Hu | 2020 | Journal of Animal Science and Biotechnology2020，11，2： | 3 |
| 5 | Autophagy-associated signal pathways of functional foods for chronic diseases显示文摘Functional foods,namely as natural or processed foods containing bioactive compounds,can provide health-promoting effects beyond basic nutrition,or offer the prevention or supplementary treatment of chronic diseases.The bioactive components in functional foods usually have pleiotropic effects,including antioxidant,anti-inflammatory,hypolipidemic,glycemic-regulating,cytoprotective,and neuroprotective functions.Autophagy is one of the highly conserved cellular processes for the clearance of aberrant components in eukaryotic cells,and plays an essential role in health promotion and prevention and treatment of a series of chronic diseases.Once the cells are in the stress environment,the induced autophagy will accelerate the clearance of cellular damaged or toxic protein aggregates or dysfunctional cellular organelles to maintain homeostasis in cells.In this article,we summarize several widely investigated bioactive components used as functional foods,such as resveratrol,epigallocatechin-3-gallate,curcumin and trehalose,with the regulatory function for autophagy during the intervention of chronic diseases,which will provide the references or novel thoughts for the development of functional foods with the modulation of autophagy. | Jinfeng Xie Jiling Liang Ning Chen | 2019 | Food Science and Human Wellness2019，8，1： | 2 |
| 6 | Resveratrol induces proliferation in preosteoblast cell MC3T3-E1 via GATA-1 activating autophagy显示文摘Resveratrol (RSV) could promote osteogenic activity, but its clinical application has been hampered in view of its poor bioavailability. Therefore, it is desirable to identify with certainty the molecular target of its bone mass boosting function, which is crucial to the design of an effective therapeutic strategy for the optimal treatment of osteoporosis. Emerging evidence has indicated that GATA-1, an important transcription factor in megakaryocyte and erythrocyte differentiation, can directly activate autophagy in erythrocytes, alluding to its impact on bone metabolism. In light of this, we sought to determine whether GATA-1 would be a putative target by which RSV would act on osteoblast proliferation and, if so, to explore the underlying mechanism involved in the process. We examined the cell viability, colony formation, cell cyclin expression, autophagy level, and the expression levels of GATA-1 and adenosine 5'-monophosphate {AMP}-activated protein kinase α {AMPKα} in osteoblastic cell strain MC3T3-E1. The results showed that RSV promoted the proliferation process in MC3T3-E1 coupled with increased expression of GATA-1. | Xiang Liu Jun Tao Yueyi Yao Ping Yang Juhui Wang Mali Yu Jianhong Hou Ying Zhang Li Gui | 2021 | Acta Biochimica et Biophysica Sinica2021，53，11： | 0 |
| 7 | 白藜芦醇抑制大鼠高原缺氧心肌损伤的作用及机制研究显示文摘目的探究白藜芦醇抑制大鼠高原缺氧心肌损伤作用及机制。方法选用8周龄雄性Wistar大鼠,将其随机分为常氧对照组(0.5%羧甲基纤维素钠,n=15)、低氧暴露组(0.5%羧甲基纤维素钠,n=15)、白藜芦醇干预组(低氧暴露+400 mg/kg白藜芦醇,n=15)。各组动物预先灌胃15 d,低氧暴露组和白藜芦醇干预组转至低压氧舱进行低氧干预(固定海拔高度6000 m,20 h/d,30 d)。小动物超声仪测定超声心动图。干预完成后,腹主动脉取血,分离心脏用于后续检测。TUNEL染色检测心肌细胞凋亡情况;DHE染色检测心脏组织活性氧(ROS)水平;TBA法和比色法检测血清超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)水平。酶联免疫吸附法测定血清中白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平。免疫荧光染色测定自噬标志蛋白LC3和线粒体外膜蛋白TOMM20。结果与常氧对照组相比,低氧暴露组大鼠心脏指数显著升高(P<0.05),心室壁增厚,心腔容积减少,射血分数和左心室缩短分数降低(P<0.05);与低氧暴露组相比,白藜芦醇干预组心脏指数显著下降(P<0.05),室壁增厚现象有所改善,心腔容积显著扩大,射血分数和左心室缩短分数显著回升(P<0.05)。与常氧对照组相比,低氧暴露组细胞凋亡率升高;与低氧暴露组相比,白藜芦醇干预组细胞凋亡显著减少,差异均有统计学意义(P<0.05)。与常氧对照组相比,低氧暴露组ROS水平显著升高;与低氧暴露组相比,白藜芦醇干预组ROS水平显著降低,差异均有统计学意义(P<0.05)。与常氧对照组相比,低氧暴露组大鼠血清中SOD,GSH-Px含量显著降低;与低氧暴露组相比,白藜芦醇干预组大鼠血清中抗氧化酶SOD,GSH-Px含量显著升高,差异均有统计学意义(P<0.05)。与常氧对照组相比,低氧暴露组大鼠血清中炎症因子IL-6、TNF-α水平显著升高;与低氧暴露组比,白藜芦醇干预组血清中IL-6、TNF-α水平显著降低,差异均有统计学意义(P<0.05)。与常氧对照组相比,低氧暴露组LC3表达量显著升高,TOMM20表达量显著降低,自噬及线粒体自噬水平显著增强;与低氧暴露组相比,白藜芦醇干预组LC3表达量降低,TOMM20表达量升高,自噬及线粒体自噬水平减弱,差异均有统计学意义(P<0.05)。结论白藜芦醇可显著缓解长期低氧暴露引发的心肌损伤,推测白藜芦醇可通过调节氧化应激介导的自噬及线粒体自噬发挥对缺氧心肌细胞的保护作用。 | 张宝怡 沈思 王新兴 郭长江 | 2023 | 中国慢性病预防与控制2023，31，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员