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    题名 作者 年代 出处 被引量
1Abscisic Acid Signaling Inhibits Bmssinosteroid Signaling through Dampening the Dephosphorylation of BIN2 by ABI1 and ABI2显示文摘Haijiao Wang Jie Tang Jing Liu Jin Hu Jingjing Liu Yuxiao Chen Zhenying Cai Xuelu Wang 2018Molecular Plant2018,11,2:17
2Response of axillary bud development in garlic(Allium sativum L.) to seed cloves soaked in gibberellic acid(GA3) solution显示文摘Gibberellins(GAs) are important phytohormones that regulate many developmental processes in plants. Clove, as the reproductive organ of garlic, dramatically affected garlic bulb development. Considering the potential of gibberellins in plant development and our previous studies, we investigated the effect of soaking two types of seed cloves(seed clove-I: without root/shoot sprouting;seed clove-II: with root/shoot sprouting) in GA3 solution on axillary bud development and examined the effect of soaking seed cloves in GA3 solution on bulb development, phytohormone level and sugar content in this study.Results indicated seed clove types, soaking liquids and their interaction significantly affected the number of cloves per bulb and the rate of single-clove bulb. Moreover, soaking seed cloves in 1 mmol L^-1 GA3 solution for 24 h not only promoted axillary bud formation and secondary plant growth(equal to tillering or branching), but also slightly increased the number of cloves per bulb and changed bulb structure with a low yield and marketable quality. On the 40 th day after GA3 treatment(at axillary bud outgrowth stage), zeatin riboside(ZR) and soluble protein in stem were sharply increased with the increase of GA3, sucrose, fructose and soluble protein in leaf. However, GA3, indole-3-acetic acid(IAA), soluble sugar and sucrose in stem(3.52 ng g^-1 fresh weight(FW), 19.88 ng g^-1 FW, 237.3 mg g^-1 FW, and 8.24 mg g^-1 FW, respectively) were significantly decreased on the 40 th day after GA3 treatment, compared to the control of water treatment(5.56 ng g^-1 FW, 32.96 ng g^-1 FW, 263.6 mg g^-1 FW, and 10.37 mg g^-1 FW, respectively). To our knowledge, these novel results indicate seed cloves soaked in GA3 solution promotes axillary bud formation and outgrowth that caused the changes in plant architecture and bulb structure. Meanwhile, our findings suggest that the level of endogenous plant hormone(GA3, IAA and ZR) cooperates with the content of sugar(sucrose and fructose) in leaf and stem to regulate axillary bud outgrowth in garlic.LIU Hong-jiu HUANG Cai-ping TONG Pei-jiang YANG Xue CUI Ming-ming CHENG Zhi-hui 2020Journal of Integrative Agriculture2020,19,4:1
3油菜素内酯调控蛋白OsCHRLK在水稻抗真菌过程中的功能显示文摘为探明油菜素内酯(BR)提高水稻(Oryza sativa)抗真菌的分子机制,采用双向电泳和质谱技术鉴定受BR调控的蛋白质,结果表明:OsCHRLK(Chitinase-Related Receptor-Like Kinase)受BR上调;通过RT-PCR技术对水稻叶片中编码几丁质酶(Chitinase)基因CHA1的表达量进行分析,发现CHA1受BR的上调;对BR处理后水稻叶片细胞间隙液中几丁质酶的活性分析表明,几丁质酶的活性也明显受BR上调;过表达OsCHRLK的转基因拟南芥表现为抗灰葡萄孢的能力增强,说明OsCHRLK在BR提高水稻抗性方面起正调控作用。以上结果表明BR可激活几丁质酶信号系统,加速对真菌细胞壁的降解,减少病菌对植物的伤害。高梦烛 耿同茹 高静 张昊 李生辉 邢继红 王凤茹 董金皋 2016河北农业大学学报2016,39,2:1
4High-Throughput MicroRNA and mRNA Sequencing Reveals that MicroRNAs May Be Involved in Pectinesterase-Mediated Cold Resistance in Potato显示文摘Since potato cultivars are sensitive to low temperature,cold injury severely affects the geographical distribution and yield of potato.Although some miRNAs have been identified in response to cold stress in plants,there is no report about the role of miRNAs in the response to cold stress in potato.Here,via high throughput sequencing,we described the profiling of cold stress response to miRNA and mRNA in potato.Two small RNA and six mRNA libraries were constructed and sequenced.296 known and 211 novel miRNAs were identified,in which 34 miRNAs in Cold Group(CG)had the higher expression quantity than which in Normal Group(NG)and 32 in CG had lower expression quantity than which in NG.3068 differentially expressed genes were detected between NG and CG,in which 1400 genes were up-regulated and 1668 genes were down-regulated.The metabolism pathway of starch and sucrose(ko00500)is the common KEGG pathway in differentially expressed miRNA and mRNA.In this pathway,StuPME21575 and StuPME42971 are pectinesterase which mainly catalyzes the pectin-forming pectate,which are controlled by stu-miR6023 and stu-novel-miR42365.As the potato suffering cold stress,these two miRNAs expression levels became higher,but their target genes expression levels were just opposite and this result is the same with qRT-PCR.Chongchong Yan Qianqian Wang Nan Zhang Jiajia Wang Xuexiang Ren Bingjie Xue Xiaojing Pu Zhihong Xu Huajun Liao 2020Phyton-International Journal of Experimental Botany2020,89,3:0
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