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| 1 | NEAT1/miR-140-3p/MAPK1 mediates the viability and survival of coronary endothelial cells and affects coronary atherosclerotic heart disease显示文摘Studies have shown that long non-coding RNAs(lncRNA)play critical roles in coronary atherosclerotic heart disease(CAD).However,the function of lncRNA nuclear enriched abundant transcript 1(NEAT1)in CAD is unclear.In this study,we aimed to investigate the functions of lncRNA NEAT1 in CAD.RTPCR and western blot analysis were carried out to examine the expressions of related RNAs.Colony formation assay,cell proliferation assay,apoptosis assay,and dual-luc讦erase reporter assay were con ducted to investigate the abilities of colony migration,cell proliferatio n,apoptosis,and targeting.The results showed that NEAT1 was up-regulated in CAD blood samples and in human coronary endothelial cells(HCAECs).Transfection of pcNEATI sign讦icantly inhibited the survival rate of HCAECs and induced apoptosis of HCAECs.MiR-140-3p was down-regulated in HCAECs.NEAT1 directly targeted miR-140-3p,and the expression of miR-140-3p was inversely correlated with the expression of NEAT1 in CAD patients.In addition,co-transfection of NEAT1 with miR-140-3p mimic reversed the effect of pcNEATI on cell viability and apoptosis,mitogenactivated protein kinase 1(MAPK1)was proved to be a target gene of miR-140-3p,and the miR-140-3p mimic was shown to reduce the expression of MAPK1 in HCAECs.pcNEATI sigrdficantly increased the expression level of MAPK1,while shNEATI significantly reduced the expression level of MAPK1.Our results revealed that lncRNA NEAT1 increased cell viability and inhibited CAD cell apoptosis possibly by activating the miR-140-3p/MAPK1 pathway,and lncRNA NEAT1 might serve as a potential therapeutic target for CAD. | Hui Zhang Ningning Ji Xinyan Gong Shimao Ni Yu Wang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，9： | 9 |
| 2 | 长链非编码RNA与性激素依赖性肿瘤显示文摘随着基因测序技术与核酸定量分析技术的发展,近年的大量研究表明,长链非编码RNA(long non-coding RNA,LncRNA)通过多种途径调控基因表达,具有调节细胞功能的重要作用。LncRNA的异常表达与肿瘤发生发展之间的联系被广泛关注。其中,关于LncRNA与3种最常见的性激素依赖性肿瘤乳腺癌、子宫内膜癌和前列腺癌的研究,揭示其在肿瘤细胞或组织中扮演着类似于原癌基因或抑癌基因的双重角色。并通过多种调控机制,参与癌细胞的侵袭、增殖、转移等过程。因性激素受体分布的特异性,使得与之相关的多种LncRNA的表达也具有较高的特异性。本文总结LncRNA与乳腺癌、子宫内膜癌和前列腺癌的相关研究进展,包括涉及到的LncRNA种类、表达差异、作用机制及作为生物标志物或治疗靶点的可行性评价。 | 赵明 杨芝芸 丁先锋 | 2016 | 中国生物化学与分子生物学报2016，32，4： | 3 |
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