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| 1 | Phot2-regulated relocation of NPH_3 mediates phototropic response to high-intensity blue light in Arabidopsis thaliana显示文摘Two redundant blue-light receptors, known as phototropins(phot1 and phot2), influence a variety of physiological responses, including phototropism, chloroplast positioning, and stomatal opening in Arabidopsis thaliana.Whereas phot1 functions in both low-and high-intensity blue light(HBL), phot2 functions primarily in HBL. Here, we aimed to elucidate phot2-specific functions by screening for HBLinsensitive mutants among mutagenized Arabidopsis phot1 mutants. One of the resulting phot2 signaling associated(p2 sa) double mutants, phot1 p2 sa2, exhibited phototropic defects that could be restored by constitutively expressing NON-PHOTOTROPIC HYPOCOTYL 3(NPH3), indicating that P2 SA2 was allelic to NPH3. It was observed that NPH3-GFP signal mainly localized to and clustered on the plasma membrane in darkness. This NPH3 clustering on the plasma High-Immembrane was not affected by mutations in genes encoding proteins that interact with NPH3, including PHOT1, PHOT2 and ROOT PHOTOTROPISM 2(RPT2). However, the HBL irradiationmediated release of NPH3 proteins into the cytoplasm was inhibited in phot1 mutants and enhanced in phot2 and rpt2-2 mutants. Furthermore, HBL-induced hypocotyl phototropism was enhanced in phot1 mutants and inhibited in the phot2 and rpt2-2 mutants. Our findings indicate that phot1 regulates the dissociation of NPH3 from the plasma membrane, whereas phot2 mediates the stabilization and relocation of NPH3 to the plasma membrane to acclimate to HBL. | Xiang Zhao Qingping Zhao Chunye Xu Jin Wang Jindong Zhu Baoshuan Shang Xiao Zhang | 2018 | Journal of Integrative Plant Biology2018，60，7： | 6 |
| 2 | 华蟾毒精对小鼠巨噬细胞功能的影响显示文摘【目的】阐明华蟾毒精(CBG)对巨噬细胞功能的影响,为CBG的开发和利用提供理论依据。【方法】利用Balb/c小鼠制备与纯化腹腔巨噬细胞,采用不同质量浓度(0.5,1.0,1.5,2.0mg/L)CBG作用于巨噬细胞,采用MTT法检测CBG对巨噬细胞吞噬金黄色葡萄球菌的影响;采用Griess法检测CBG对巨噬细胞分泌NO的影响;采用ELISA法检测CBG对巨噬细胞分泌IL-6、TNF-α和GM-CSF的影响。【结果】CBG质量浓度为1.0,1.5和2.0mg/L组均能极显著提高巨噬细胞的吞噬功能;CBG质量浓度为0.5 mg/L组能显著促进巨噬细胞NO的分泌,而1.0,1.5和2.0mg/L CBG组均能极显著促进巨噬细胞NO的分泌;CBG质量浓度为1.0,1.5和2.0mg/L组均能极显著促进巨噬细胞IL-6的分泌;1.0mg/L CBG组能显著促进巨噬细胞TNF-α的分泌,1.5和2.0mg/L CBG组均能极显著促进巨噬细胞TNF-α的分泌;CBG质量浓度为0.5,1.0,1.5和2.0mg/L组均能极显著促进巨噬细胞GM-CSF的分泌。【结论】CBG能提高巨噬细胞的吞噬功能和分泌功能,说明CBG具有良好的抗炎、抗肿瘤和提高免疫等作用。 | 孟显华 于洋 郝璐 刘天明 付越 李敬双 | 2016 | 西北农林科技大学学报(自然科学版)2016，44，8： | 1 |
| 3 | AtRGS1胞吞动态调控G蛋白参与拟南芥生长发育和抗性反应显示文摘异源三聚体G蛋白由Gα、Gβ和Gγ 3个亚基组成,是普遍存在于真核细胞中的跨膜信号转导因子。植物细胞通过定位于细胞质膜的G蛋白信号调节子RGS蛋白(regulator of G protein signaling),调控异源三聚体G蛋白的活性,进而参与生长发育、激素和糖信号转导以及抗病反应等多个重要生物学过程。膜蛋白可通过胞吞循环调控其在细胞质膜上的数量,以响应外界环境因子和自身发育信号。近年来,研究表明多种外界信号诱导拟南芥AtRGS1蛋白的胞吞,进而促进其与Gα亚基的解离,游离的Gα-GTP、Gβγ亚基和定位于内含体的AtRGS1蛋白均可能调控下游信号转导,进而影响相应生物学过程。本文综述了AtRGS1通过胞吞作用调控G蛋白参与的生长发育和抗性反应的分子细胞学机制研究进展,以期为深入理解G蛋白信号调节子影响植物发育进程和抗性反应的作用机制提供理论参考,为植物膜蛋白胞吞调控信号转导提供新的视角。 | 古盼 齐学影 李莉 张曦 单晓昳 | 2022 | 生物技术通报2022，38，6： | 0 |
| 4 | Cell Polarity and Development显示文摘All cells show some degree of polarity,either by asymmetrically distributed membrane or cytosolic components.Even in bacterial cells that do not have the eukaryotic membrane compartmentalization of the cytoplasm,proteins can be localized at specifc areas.In rod‐shaped bacteria,many processes such as signaling,fagella formation,and DNA uptake occur at the cell poles.In addition,the symmetry of cell division in | Remko Offringa Jürgen Kleine‐Vehn | 2013 | Journal of Integrative Plant Biology2013，55，9： | 0 |
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