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| 1 | Ras信号通路通过激活转录因子Myc促进核内复制细胞生长显示文摘【目的】果蝇Ras信号通路在细胞增殖与生长过程中发挥着重要的作用。Myc基因是bHLH转录因子家族基因,可调控细胞生长、竞争和再生增殖等生理过程。本研究旨在明确Ras信号通路与Myc的关系,探索Ras信号调控核内复制细胞生长的作用机制。【方法】生物信息学分析转基因家蚕Bombyx mori后部丝腺Myc基因的转录水平,并通过qPCR验证;在黑腹果蝇Drosophila melanogaster Kc细胞中,分别转染pAc5.1-HisB-Ras^(V12)-V5或pAc5.1-HisB-Raf-Flag过表达Ras^(V12)或Raf后,通过qPCR和Western blot技术分别检测Myc基因在mRNA和蛋白水平的相对表达量;在黑腹果蝇幼虫脂肪体和唾液腺中,结合黑腹果蝇遗传工具和分子生物学手段,验证Ras信号通路对Myc基因的调控作用。【结果】家蚕后部丝腺过表达Ras1^(CA)上调Myc转录水平。激活Ras信号使得黑腹果蝇Kc细胞内Myc在转录水平和蛋白水平上的表达量上调;黑腹果蝇幼虫唾液腺和脂肪体中,游走期Myc基因的表达量高于取食期;过表达Myc或激活Ras信号可以促进细胞核内周期进程;激活Ras信号促进Myc表达。【结论】Ras信号通路激活Myc表达,促进细胞核内周期进程,促进器官发育。 | 张祥乐 马俐 马倩 李胜 刘素宁 | 2018 | 昆虫学报2018，61，8： | 2 |
| 2 | Dual role of FGF in proliferation and endoreplication of Drosophila tracheal adult progenitor cells显示文摘Adult progenitor cells activation is a key event in the formation of adult organs.In Drosophilat formation of abdominal adult trachea depends on the specific activation of tracheal adult progenitors(tracheoblasts)at the Tr4 and Tr5 spiracular branches.Proliferation of these tracheoblasts generates a pool of tracheal cells that migrate toward the posterior part of the trachea by the activation of the branchless/fibroblast growth factor(Bnl/FGF)signaling to form the abdominal adult trachea.Here,we show that,in addition to migration,Bnl/FGF signaling,mediated by the transcription factor Pointed,is also required for tracheoblast proliferation.This tracheoblast activation relies on the expression of the FGF ligand bnl in their nearby branches.Finally,we show that,in the absence of the transcription factor Cut(Ct),Bnl/FGF signaling induces endoreplication of tracheoblasts partially by promoting fizzy-related expression.Altogether,our results suggest a dual role of Bnl/FGF signaling in tracheoblasts,inducing both proliferation and endoreplication,depending on the presence or absence of the transcription factor Ct,respectively. | Cristina de Miguel Josefa Cruz David Martin Xavier Franch-Marro | 2020 | Journal of Molecular Cell Biology2020，12，1： | 0 |
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