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1Whole-genome sequencing of leopard coral grouper(Plectropomus leopardus) and exploration of regulation mechanism of skin color and adaptive evolution显示文摘Leopard coral groupers belong to the Plectropomus genus of the Epinephelidae family and are important fish for coral reef ecosystems and the marine aquaculture industry. To promote future research of this species, a high-quality chromosome-level genome was assembled using PacBio sequencing and Hi-C technology. A 787.06 Mb genome was assembled, with 99.7%(784.57 Mb) of bases anchored to 24 chromosomes. The leopard coral grouper genome size was smaller than that of other groupers, which may be related to its ancient status among grouper species. A total of 22 317 proteincoding genes were predicted. This high-quality genome of the leopard coral grouper is the first genomic resource for Plectropomus and should provide a pivotal genetic foundation for further research. Phylogenetic analysis of the leopard coral grouper and 12 other fish species showed that this fish is closely related to the brown-marbled grouper.Expanded genes in the leopard coral grouper genome were mainly associated with immune response and movement ability, which may be related to the adaptive evolution of this species to its habitat. In addition, we also identified differentially expressed genes(DEGs) associated with carotenoid metabolism between red and brown-colored leopard coral groupers. These genes may play roles in skin color decision by regulating carotenoid content in these groupers.Yang Yang Li-Na Wu Jing-Fang Chen Xi Wu Jun-Hong Xia Zi-Ning Meng Xiao-Chun Liu Hao-Ran Lin 2020Zoological Research2020,41,3:2
2海南野生豹纹鳃棘鲈染色体核型及银染研究显示文摘以野生豹纹鳃棘鲈Plectropomus leopardus为材料,采用抽血、植物血球凝集素(PHA)和秋水仙素体腹腔注射法,取头肾细胞制备染色体标本,对其染色体核型和Ag-NORs进行了分析。结果表明:豹纹鳃棘鲈二倍体染色体数目为48,其核型公式为2n=2st(t)+46t,NF=48;仅有1对NORs位于第1对染色体长臂靠近着丝粒的位置。高宁 蔡岩 周永灿 李根浪 袁卫 刘海天 谢珍玉 王世锋 2015大连海洋大学学报2015,30,3:0
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