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1不同地域野生欧李及其近缘植物亲缘关系的RAPD分析显示文摘利用RAPD分子标记技术对21个不同地域欧李及其近缘种属植物(桃、杏、李、樱桃)进行了遗传亲缘关系及分类研究。结果表明,用于不同地域野生欧李分析的25条引物中,共扩增出441个清晰可用的DNA片段,其中多态性位点366个,多态性比为82.99%。在遗传相似系数为0.71时,可将中国野生欧李资源划分为3个大的区域,分别为东北分布群、华北华东分布群与中西部分布群。用于欧李及其近缘种属植物分析的20条引物中,共扩出多态性带683条,多态性比率为98.99%。聚类分析表明,在遗传相似系数为0.61时,欧李与其近缘种属植物分为3大类;欧李与麦李的亲缘关系最近,与桃亲缘关系最远。王鹏飞 李利锋 杜俊杰 张建成 纪薇 穆霄鹏 曹琴 2015植物遗传资源学报2015,16,1:13
2Genetic diversity analysis of Sinojackia microcarpa, a rare tree species endemic in China, based on simple sequence repeat markers显示文摘Although most Sinojackia species are endangered, they contribute greatly to the biodiversity of local ecosystems. Sinojackia microcarpa, an endangered species, is distributed only in three provinces of eastern China. Determining the genetic diversity of S. microcarpa provides key information for germplasm evaluation and species conservation. Here we used simple sequence repeat (SSR) markers to investigate the genetic diversity of eight natural populations of S. microcarpa. Leaf samples were collected from 144 individuals in 8 wild populations. The 156 bands were generated from 14 pairs of informative SSR primers, with an average percentage of polymorphic bands of 45.67%. The average values of Nei’s genetic diversity (He) and Shannon’s diversity index (I) were 0.1007 and 0.1658, respectively. The total genetic variation of S. microcarpa existed mainly within the eight populations, rather than among populations, and reached 86.41%. A cluster analysis showed that the eight wild populations of S. microcarpa could be classified into four groups, at a threshold of 4.0, based on an analysis of the SSR genotypes. Furthermore, there was a significant association between the phylogenetic relationships and the geographic locations of the S. microcarpa populations. In particular, populations from Fuyang, Jiande, and Lin’an in Zhejiang Province had close phylogenetic relationships and geographic distances. In addition, these three populations had the highest genetic diversity and the most individuals, suggesting that these three locations may be the S.microcarpa distribution center. This study serves as a model for studying the genetic diversity of endangered plant species.Tailin Zhong Guangwu Zhao Yongfeng Lou Xinchun Lin Xiaomin Guo 2019Journal of Forestry Research2019,30,3:2
3Biotechnology of the multipurpose tree species Arbutus unedo:a review显示文摘Arbutus unedo L.(strawberry tree,Ericaceae)is a woody species with a circum-Mediterranean distribu-tion.It has considerable ecological relevance in southern European forests due to its resilience to abiotic and biotic stressors.Its edible red berries are used in the production of traditional products,including an expensive spirit.Several compounds extracted from the species have bioactive prop-erties used by the cosmetic and pharmaceutical industries.The strawberry tree has gone from a neglected species to become a highly valuable crop with large cultivated areas in southern European and North African countries.Due to an increasing demand from farmers for plants with improved features,researchers have been trying to improve this spe-cies through conventional and biotechnological tools,focus-ing mainly on population analysis using molecular markers,in vitro cloning,tolerance to biotic and abiotic stresses,and intraspecific crosses to obtain genotypes with new char-acteristics.The objective of this review is to gather and update information about the species and make it available to researchers and stakeholders.Future research areas that are considered a priority for this species are highlighted.João Martins Glória Pinto Jorge Canhoto 2022Journal of Forestry Research2022,33,2:0
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