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| 1 | 抗生素诱导的小鼠肠动力减弱与肠胶质细胞过度活化相关显示文摘目的探讨抗生素处理小鼠肠动力与肠胶质细胞活化的关系。方法通过联合抗生素处理获得低肠道菌群的小鼠,通过胭脂红溶液灌胃后记录色素排出时间以及相同时间内排出粪便颗粒,比较抗生素组与对照组小鼠的肠动力;采用免疫荧光技术比较两组小鼠肌间神经丛内胶质细胞与神经元的形态与分布,采用实时荧光定量PCR检测两组小鼠结肠与回肠组织内肠胶质细胞活化与功能相关的基因胶质纤维酸性蛋白(GFAP)、胶质细胞源性神经营养因子(GDNF)、 S100B、神经生长因子(NGF)的mRNA水平,采用流式细胞术检测小鼠肠黏膜固有层淋巴细胞内调节性T细胞(Treg)和分泌白细胞介素17(IL-17)的Th17细胞的比例。结果抗生素处理后的小鼠肠道菌群数量显著降低,盲肠体积膨大,结肠萎缩变短,肠动力减弱,结肠内胶质细胞GFAP、 GDNF、 S100B、 NGF的mRNA水平增加,而结肠黏膜固有层内Treg和Th17细胞比例均降低。结论抗生素处理后肠动力的降低可能与肠胶质细胞的过度活化相关,且抗生素处理后肠胶质细胞的活化与肠道炎症无关。 | 谢韬 加潇坤 陈木彬 | 2019 | 细胞与分子免疫学杂志2019，35，3： | 6 |
| 2 | 胶囊内镜用于小肠疾病的诊断价值显示文摘目的探讨胶囊内镜在小肠疾病诊断中的应用及价值。方法对2010年3月至2016年10月到本院行胶囊内镜检查的疑似小肠疾病患者95例(试验组)和体检者21例(对照组)进行临床分析,评估胶囊内镜在小肠疾病诊断中的价值。结果检查过程中受检者耐受性较好。胶囊内镜在胃内运行时间为55.71±6.55 min,在小肠内运行时间259.54±94.63 min。小肠疾病总体检出率为43.1%(50/116),试验组小肠疾病检出率为47.4%(45/95),显著高于对照组的23.8%(5/21)(P<0.05)。结论胶囊内镜对小肠疾病具有较高的诊断价值。 | 朱骊 俞宪民 林周 | 2018 | 热带病与寄生虫学2018，16，4： | 4 |
| 3 | 双气囊小肠镜在小肠常见疾病中的临床应用显示文摘双气囊小肠镜(double-balloon enteroscopy,DBE)的临床应用,能够到达传统内镜检查的'盲区'。相对于传统的检查手段,DBE在小肠常见疾病中有很高的诊断率,同时可行镜下取活体组织送检病理及治疗,高效安全。本文就DBE在小肠常见疾病中的临床应用价值研究方面的进展作一概述。 | 奚腾飞 徐洪雨 | 2014 | 胃肠病学和肝病学杂志2014，23，12： | 3 |
| 4 | Clinical relevance of intestinal peptide uptake显示文摘AIM: To determine available information on an independent peptide transporter 1(Pep T1) and its potential relevance to treatment, this evaluation was completed.METHODS: Fully published English language literature articles sourced through Pub Med related to protein digestion and absorption, specifically human peptide and amino acid transport, were accessed and reviewed.Papers from 1970 to the present, with particular emphasis on the past decade, were examined. In addition,abstracted information translated to English in Pub Med was also included. Finally, studies and reviews relevant to nutrient or drug uptake, particularly in human intestine were included for evaluation. This work represents a summary of all of these studies with particular reference to peptide transporter mediated assimilation of nutrients and pharmacologically active medications.RESULTS: Assimilation of dietary protein in humans involves gastric and pancreatic enzyme hydrolysis to luminal oligopeptides and free amino acids. During the ensuing intestinal phase, these hydrolytic products are transported into the epithelial cell and, eventually, the portal vein. A critical component of this process is the uptake of intact di-peptides and tri-peptides by an independent Pep T1. A number of 'peptide-mimetic' pharmaceutical agents may also be transported through this carrier, important for uptake of different antibiotics, antiviral agents and angiotensin-converting enzyme inhibitors. In addition, specific peptide products of intestinal bacteria may also be transported by Pep T1, with initiation and persistence of an immune response including increased cytokine production and associated intestinal inflammatory changes. Interestingly, these inflammatory changes may also be attenuated with orallyadministered anti-inflammatory tripeptides administered as site-specific nanoparticles and taken up by this Pep T1 transport protein. CONCLUSION: Further evaluation of the role of this transporter in treatment of intestinal disorders, including inflammatory bowel disease is needed. | Hugh James Freeman | 2015 | World Journal of Gastrointestinal Pharmacology and Therapeutics2015，6，2： | 1 |
| 5 | 六味降脂汤对高脂血症大鼠肠道菌群及小肠NPC1L 1、ACAT2和APOB48蛋白表达的影响显示文摘目的:探究六味降脂汤对高脂血症可能的干预机制以及对肠道菌群的影响。方法:60只SPF级SD雄性大鼠随机分为正常对照组、模型对照组、阿托伐他汀0.9 mg/kg组及六味降脂汤5.4、10.7、21.4 g/kg。除正常对照组外,其余各组给予高脂饲料合并高脂乳剂灌胃6 w制备高脂血症大鼠模型,造模成功各组灌胃给予相应药物,8 w后采集血清、粪便及小肠组织样本,检测大鼠血清中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)含量;同时采用16S rDNA高通量测序分析肠道菌群的多样性和结构变化;免疫组织化学法检测大鼠小肠组织NPC1L1、ACAT2及APOB48蛋白表达。结果:与正常对照组比较,模型对照组大鼠血清TC、TG、LDL-C含量显著升高,小肠NPC1L1、ACAT2及APOB48蛋白表达显著上调(P<0.01),肠道菌群Beta多样性改变;与模型对照组比较,各给药组TC、TG、LDL-C含量均明显降低(P<0.05或P<0.01),六味降脂汤10.7 g/kg组小肠NPC1L1、ACAT2及APOB48蛋白表达明显下调(P<0.05或P<0.01),肠道菌群结构改变,在门水平上,厚壁菌门(Firmicutes)相对丰度明显降低,拟杆菌门(Bacteroidetes)相对丰度明显升高(P<0.05或P<0.01);属水平上,布劳特氏菌属(Blautia)相对丰度明显升高,乳杆菌属(Lactobacillus)及Ruthenibacterium相对丰度明显降低(P<0.05或P<0.01)。结论:六味降脂汤可能通过下调小肠NPC1L1、ACAT2和APOB48蛋白的表达,影响肠道微生物群,调节高脂血症大鼠体内脂质代谢,从而达到降脂作用。 | 杜京晏 张远哲 蔡琨 谢珂 全德森 田维毅 黎豫川 | 2023 | 中药药理与临床2023，39，6： | 0 |
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