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| 1 | BnWRI1 coordinates fatty acid biosynthesis and photosynthesis pathways during oil accumulation in rapeseed显示文摘Photosynthesis in 'green' seeds, such as rapeseed,soybean, and Arabidopsis, plays a substantial role in the improved efficiency of oil accumulation. However, the molecular mechaResearchnism underpinning the coordinated expression of fatty acid(FA)biosynthesis- and photosynthesis-related genes in such developing seeds remains to be elucidated. Here, we found that seed-specific overexpression of BnWRI1, a WRI1 homolog from rapeseed(Brassica napus cv. ZGY2), results in enhanced chlorophyll content in developing seeds and increased oil content and seed mass in matured seeds. BnWRI1 was co-expressed with BnBCCP and BnCAB, two marker genes of FA biosynthesis and photosynthesis during seed development, respectively. Overexpression of BnWRI1 increased expression of both marker genes. Further, the nuclear-localized BnWRI1 protein was found to act as a transcription activator. It could bind to the GT1-element and/or GCC-box, which are widespread in the upstream regions of genes involved in FA biosynthesis and photosynthesis pathways.Accordingly, BnWRI1 could interact with promoters of BCCP2 and LHB1B2 in vivo. These results suggested that BnWRI1 may coordinate FA biosynthesis and photosynthesis pathways in developing seeds via directly stimulating expression of GT1-element and/or GCC-box containing genes. | Xue-Long Wu Zhi-Hong Liu Zhang-Hua Hu Rui-Zhi Huang | 2014 | Journal of Integrative Plant Biology2014，56，6： | 9 |
| 2 | Construction and validation of a gene co-expression network ingrapevine (Vitis vinifera. L.)显示文摘Gene co-expression analysis has been widely used for predicting gene functions because genes within modules of a co-expression network may be involved in similar biological processes and exhibit similar biological functions.To detect gene relationships in the grapevine genome,we constructed a grapevine gene co-expression network(GGCN)by compiling a total of 374 publically available grapevine microarray datasets.The GGCN consisted of 557 modules containing a total of 3834 nodes with 13479 edges.The functions of the subnetwork modules were inferred by Gene ontology(GO)enrichment analysis.In 127 of the 557 modules containing two or more GO terms,38 modules exhibited the most significantly enriched GO terms,including‘protein catabolism process’,‘photosynthesis’,‘cell biosynthesis process’,‘biosynthesis of plant cell wall’,‘stress response’and other important biological processes.The‘response to heat’GO term was highly represented in module 17,which is composed of many heat shock proteins.To further determine the potential functions of genes in module 17,we performed a Pearson correlation coefficient test,analyzed orthologous relationships with Arabidopsis genes and established gene expression correlations with real-time quantitative reverse transcriptase PCR(qRT-PCR).Our results indicated that many genes in module 17 were upregulated during the heat shock and recovery processes and downregulated in response to low temperature.Furthermore,two putative genes,Vit_07s0185g00040 and Vit_02s0025g04060,were highly expressed in response to heat shock and recovery.This study provides insight into GGCN gene modules and offers important references for gene functions and the discovery of new genes at the module level. | Ying-Hai Liang Bin Cai Fei Chen Gang Wang Min Wang Yan Zhong Zong-Ming(Max)Cheng | 2014 | Horticulture Research2014，1，1： | 4 |
| 3 | Restriction of iron loading into developing seeds by a YABBY transcription factor safeguards successful reproduction in Arabidopsis显示文摘Iron(Fe)storage in plant seeds is not only necessary for seedling establishment following germination but is also a major source of dietary Fe for humans and other animals.Accumulation of Fe in seeds is known to be low during early seed development.However,the underlying mechanism and biological significance remain elusive.Here,we show that reduced expression of Arabidopsis YABBY transcription factor INNER NO OUTER(INO)increases embryonic Fe accumulation,while transgenic overexpression of INO results in the opposite effect.INO is highly expressed during early seed development,and decreased INO expression increases the expression of NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 1(NRAMP1),which encodes a transporter that contributes to seed Fe loading.The relatively high embryonic Fe accumulation conferred by decreased INO expression is rescued by the nramp1 loss-of-function mutation.We further demonstrated that INO represses NRAMP1 expression by binding to NRAMP1-specific promoter region.Interestingly,we found that excessive Fe loading into developing seeds of ino mutants results in greater oxidative damage,leading to increased cell death and seed abortion,a phenotype that can be rescued by the nramp1 mutation.Taken together,these results indicate that INO plays an important role in safeguarding reproduction by reducing Fe loading into developing seeds by repressing NRAMP1 expression. | Li Sun Yun Qi Wei Kang Hao Wu Jing Ying Yan Jie Na Xu Yun Rong Wu Gui Xin Li Ji Ming Xu Nicholas P.Harberd Zhong Jie Ding Shao Jian Zheng | 2021 | Molecular Plant2021，14，10： | 3 |
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