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| 1 | Drought Responses of Leaf Tissues from Wheat Cultivars of Differing Drought Tolerance at the Metabolite Level显示文摘干旱在谷物庄稼的生理学上有严肃的效果。在细胞并且明确地代谢物水平,许多单个混合物被增加提供 osmoprotective 功能,并且减少,阻止酶的分离在房间在场的反应的氧种类的数字。我们使用了一条指向的 GCMS 途径识别在忍耐的不同层次在干旱应力下面描绘到干旱的面包小麦的三不同栽培变种不同的混合物(反曲刀的一种,偏狭;Excalibur 和 RAC875,容忍) 。氨基酸,最尤其是脯氨酸,色氨酸,和分叉的链氨基酸白氨酸,异白氨酸,和缬氨酸的层次在所有栽培变种在干旱应力下面被增加。在二容忍的栽培变种,在很多器官的酸的小减少也是明显的。Excalibur,栽培变种 genotypically 与反曲刀的一种有关,显示出更类似于在浇得好的条件下面的反曲刀的一种的反应的一个模式。在干旱应力下面, Excalibur 和 RAC875 有类似的回答;然而, Excalibur 没有象 RAC875 的反应的一样的大小。这里,结果在以前的工作的上下文被讨论在生理并且在干旱应力下面的这些栽培变种的 proteomic 分析。 | Jairus B. Bowne Tim A. Erwin Juan Juttner Thorsten Schnurbusch Peter Langridge Antony Bacic Ute Roessner | 2012 | Molecular Plant2012，5，2： | 25 |
| 2 | 超低温保存花粉技术在温室玉米加代育种上的应用显示文摘授粉是作物品种改良的重要技术环节。本研究对玉米花粉超低温保存过程中的干燥、液氮保存、快速解冻和精量授粉等技术环节进行了研究,并对超低温保存花粉技术在温室玉米加代育种上应用的可行性进行了分析。结果表明:花粉相对含水量是影响玉米花粉超低温保存成功的主要因素,其中玉米花粉超低温保存的最适宜相对含水量为11.3%~14.7%。超低温保存玉米花粉为温室玉米加代的顺利开展提供了保障。 | 孙果忠 | 2013 | 河北农业科学2013，17，1： | 8 |
| 3 | 植物响应温度胁迫的代谢组学研究进展显示文摘在极端温度胁迫下,植物体内会发生一系列复杂的生理、生化变化来响应外界环境。代谢组学已成为系统生物学的重要组成部分之一,是对某一生物、组织或细胞中小分子量的代谢产物进行定性和定量分析,以揭示生命现象与过程。综述了植物响应温度胁迫的代谢谱分析,并展望了代谢组学急需解决的问题。这将为今后引种、驯化、改良和培育抗寒或耐热植物提供充分的理论依据。 | 周连玉 李园媛 王文妮 钟松 | 2017 | 山西农业科学2017，45，2： | 6 |
| 4 | Effect of water deficiency on relationships between metabolism,physiology,biomass,and yield of upland cotton(Gossypium hirsutum L.)显示文摘Drought is a common abiotic stress that considerably limits crop production. The objective of this study is to explore the influence of water deficiency on the yield, physiologic and metabolomic attributes in upland cotton cultivars(Gossypium hirsutum L). Cotton cultivars, 'Ishonch' and 'Tashkent-6' were selected to study the relationships among their physiologic, metabolomic and yield attributes during water deficiency. Deficit irrigation was designed by modifying the traditional watering protocol to reduce water use. Results indicate that cotton cultivars respond differently to water deficit stress. Water deficit significantly influenced plant height, the number of internodes, and sympodial branches in both cultivars. However, yield components such as the number of bolls, boll seed, lint mass, and individual plant yield were significantly reduced only in 'Tashkent-6'. The leaf area decreased and the specific leaf weight increased in 'Ishonch' under deficit irrigation conditions. However, 'Tashkent-6' demonstrated significant water loss compared to 'Ishonch', and both cultivars showed reduced transpiration rates. Untargeted metabolite profiles of leaves showed clear separation in 'Ishonch', but not in 'Tashkent-6' under deficit irrigation compared to full irrigation. The individual metabolites such as proline and galactinol showed strong association with yield under water deficit stress. Moreover, this study indicates that leaf area and transpiration intensity influence yield during water deficiency. In summary, the correlation among morpho-physiologic, metabolic, and yield components significantly varied between the two cultivars under water deficiency. The flowering stage was sensitive to water stress for both cultivars. The direct relationship between physiology, metabolism, and yield may be a useful selection criterion for determining candidate parents for cotton drought tolerance breeding. | Tohir A BOZOROV Rustam M USMANOV YANG Honglan Shukhrat A HAMDULLAEV Sardorbek MUSAYEV Jaloliddin SHAVKIEV Saidgani NABIEV ZHANG Daoyuan Alisher A ABDULLAEV | 2018 | Journal of Arid Land2018，10，3： | 5 |
| 5 | Catabolism of Branched Chain Amino Acids Supports Respiration but Not Volatile Synthesis in Tomato Fruits显示文摘分支链氨基酸 transaminases (BCAT ) 在分支链氨基酸白氨酸,异白氨酸,和缬氨酸的新陈代谢有一个关键角色。这些酶催化合成的最后步和这些氨基酸的降级的起始的步。尽管在植物的分叉的链氨基酸的 biosynthetic 小径广泛地被调查了,很多基因被描绘了,他们在植物的分解代谢完全还没被理解。我们以前在西红柿描绘了分叉的链氨基酸 transaminase 基因家庭,揭示 subcellular 本地化和六基因编码的酶的运动性质。这里,我们在水果开发期间检验了酶的可能的功能。我们进一步描绘了在分叉的链氨基酸 transaminases 的表示不同的转基因的植物 1 和 3,在线 overexpressing 任何一个 BCAT1 或 BCAT3 在在 BCAT1 和 volatiles 的层次缺乏的水果评估呼吸的率。我们测试的份量上经由喂实验的先锋和同位素,在水果 volatiles 的形成的分叉的链氨基酸和他们的相应 keto 酸的重要性。我们的结果不仅第一次在水果呼吸表明分叉的链氨基酸的重要性,而且揭示那 keto 酸,而非氨基酸,是为分叉的链风味 volatiles 的可能的先锋。 | Andrej Kochevenko Wagner L. Araujo Gregory S. Maloney Denise M. Tieman Phuc Thi Do Mark G. Taylor Harry J. Klee Alisdair R. Fernie | 2012 | Molecular Plant2012，5，2： | 4 |
| 6 | 基于颜色和纹理特征的玉米干旱识别显示文摘探究以颜色和纹理为特征,以神经网络建模为方法,识别玉米干旱的效果。利用可见光成像方式采集不同干旱胁迫下的玉米图像,通过编程从玉米图像中自动提取颜色和纹理特征变量,以多个BP神经网络集成学习的方法构建玉米不同生长发育阶段的干旱识别模型,识别不同干旱程度的玉米植株。结果表明:玉米出苗—拔节阶段的模型在训练和验证时的平均识别准确率和平均识别精度均在90%以上,识别误差均值小于0.1;拔节—抽雄阶段的模型在训练和验证时识别干旱的平均准确率和平均识别精度均在85%以上,识别误差均值为0.1和0.14;抽雄—成熟阶段的模型在训练和验证时识别干旱的平均准确率分别为85.36%和84.27%,平均精度在均在80%以上,识别误差均值为0.15和0.16。玉米出苗—拔节、拔节—抽雄、抽雄—开花3个阶段的干旱识别模型对田间玉米干旱的平均识别准确率在75%左右,平均识别精度在80%以上,平均识别误差依次为0.22、0.21、0.29。总之,出苗—拔节阶段的玉米干旱识别模型识别干旱的能力最强,拔节—抽雄阶段的模型次之,抽雄—成熟阶段的模型较差,同一模型对同一干旱程度的识别,模型训练时的识别效果最好,验证时的识别效果次之,测试效果较差,同一模型对不同干旱程度的识别,总体表现为对中旱水平识别效果最不理想,对适宜和特旱水平的识别效果最好。研究结果为玉米干旱特征模式识别和利用表型特征实现玉米旱情自动监测预警提供参考。 | 岳焕然 李茂松 安江勇 | 2018 | 中国农学通报2018，34，24： | 3 |
| 7 | 地膜覆盖对黄土高原地区两种种植密度下玉米叶片代谢组的影响显示文摘在黄土高原地区,对覆膜条件下玉米叶片代谢组变化规律的研究是探索覆膜增产生理机制的重要方面。本研究采用气相色谱-四极杆-飞行时间质谱(GC-QTOF)技术,对2种覆膜方式(塑料膜覆盖和不覆盖)、2个密度(7.5×10^(4)株hm^(–2)和10.5×10^(4)株hm^(–2))和2个品种(郑单958和先玉335)的吐丝期玉米叶片进行了代谢组学分析。从差异代谢物个数来看,先玉335对覆膜的响应大于郑单958,较高的种植密度缩小了覆膜与无覆膜处理间叶片代谢的差异。主成分分析表明,覆膜处理和品种均对代谢物组成产生了重大影响。覆膜引起的代谢谱差异主要是由柠檬酸等有机酸和氨基酸造成的;品种引起的代谢组差异主要是由辛酸等烷酸和酚类造成的。相关分析表明,叶绿醇、白藜芦醇、葡萄糖-6-磷酸与玉米籽粒产量呈显著正相关关系,而甘油与之呈显著负相关关系。在覆膜条件下,与呼吸作用及清除光呼吸产物相关的缬氨酸、异亮氨酸及蛋氨酸,与三羧酸循环相关的异柠檬酸以及可减轻光抑制的蛋氨酸、N-乙酰基天冬氨酸等代谢物的水平整体呈升高趋势。以上结果表明,在覆膜条件下,消除光呼吸产物及减少光抑制的相关代谢物的积累是叶片净光合速率提高的代谢基础;抗氧化及能量代谢相关的代谢物在提高产量的过程中发挥了重要作用。 | 牛丽 白文波 李霞 段凤莹 侯鹏 赵如浪 王永宏 赵明 李少昆 宋吉青 周文彬 | 2021 | 作物学报2021，47，8： | 3 |
| 8 | Performance and yield stability of maize hybrids in stress-prone environments in eastern Africa显示文摘Identification and deployment of high-yielding and stress-tolerant maize hybrids adapted to stress-prone agro-ecologies is important for improving the food security and livelihoods of smallholder farmers in eastern Africa.The objectives of this study were to(i)assess the performance of maize hybrids under well-watered and drought stress conditions;(ii)evaluate grain yield stability of 65 intermediate-maturing and 55 early-maturing hybrids in 24 well-watered locations and seven drought stress locations;and(iii)identify representative and/or discriminative testing locations for increasing genetic gains for the target traits.There were significant differences for grain yield among early-and intermediatematuring hybrids tested under well-watered and drought stress environments.Among the early-maturing hybrids,the top 10 hybrids produced 46.8%–73.9%and 31.2%–42.1%higher mean grain yields than the best commercial check under drought and well-watered conditions,respectively.Among the intermediate-maturing hybrids,the top 10 hybrids produced 25.2%–47.7%and 8.5%–13.5%higher grain yield than commercial checks under drought stress and well-watered conditions,respectively,suggesting improvement in the levels of drought tolerance in both early-and intermediate-maturing hybrids.GGE biplot analysis and a bi-segmented regression linear method identified specific early-maturing and intermediate-maturing hybrids that performed well under both well-watered and drought stress conditions.These hybrids could be recommended for commercial production in eastern Africa.Kakamega in Kenya was found to be the most representative and highly discriminating site among well-watered testing locations,while Kabuku in Tanzania was the least representative of test locations.For testing under drought stress conditions,Kiboko in Kenya was identified as the most representative location.This information could be useful for allocating resources and streamlining CIMMYT maize hybrid testing in eastern Africa. | Wender Santos Rezende Yoseph Beyene Stephen Mugo Eric Ndou Manje Gowda Julius Pyton Sserumaga Godfrey Asea Ismail Ngolinda McDonald Jumbo Sylvester O.Oikeh Michael Olsen Aluízio Borém Cosme Damião Cruz Boddupalli M.Prasanna | 2020 | The Crop Journal2020，8，1： | 2 |
| 9 | 油茶高产与低产无性系代谢表达谱标记鉴别显示文摘于2020年,在福建省沙县水南国有林场的油茶无性系评比试验林中选择3株高产油茶树和3株低产油茶树,采用GC-MS技术开展与油茶产量性状相关的代谢表达谱分析与代谢标记鉴定。研究结果表明:从高产组和低产组代谢表达谱中筛选出2种含量达显著性差异的化合物:角鲨烯的相对含量高产组为4.07%,低产组为35.87%,低产组显著高于高产组(P<0.01);保留时间为17.227 min的化合物A在高产组中相对含量为31.85%,而在低产组中没有检测到。这些代谢表达物可能作为油茶优良无性系选择的代谢标记,为油茶高产品种的选择提供新方法。 | 曹汉洋 何祯祥 李志真 卢世明 郑登耿 陈耀升 赖标祯 | 2021 | 福建林业科技2021，48，4： | 1 |
| 10 | Translate Plant Metabolism into Modern Agriculture: A Starting Point显示文摘 | Xiao-Ya Chen Lai-Geng Li Ji-Rong Huang Yong-Ling Ruan Xue-Min Wang Li Li | 2012 | Molecular Plant2012，5，2： | 0 |
| 11 | Arabidopsis PED_2 positively modulates plant drought stress resistance显示文摘Abscisic acid(ABA) is an important phytohormone that functions in seed germination,plant development,and multiple stress responses.Arabidopsis Peroxisome defective 2(AtPED2)(also known as AtPEXOX/N14,AtPEX14),is involved in the intracellular transport of thiolase from the cytosol to glyoxysomes,and perosisomal matrix protein import in plants.In this study,we assigned a new role for AtPED2in drought stress resistance.The transcript level of AtPED2was downregulated by ABA and abiotic stress treatments.AtPED2 knockout mutants were insensitive to ABA-mediated seed germination,primary root elongation,and stomatal response,while AtPED2 over-expressing plants were sensitive to ABA in comparison to wide type(WT).AtPED2 also positively regulated drought stress resistance,as evidenced by the changes of water loss rate,electrolyte leakage,and survival rate.Notably,AtPED2 positively modulated expression of several stress-responsive genes(RAB18,RD22,RD29 A,and RD29B),positively affected underlying antioxidant enzyme activities and negatively regulated reactive oxygen species(ROS) level under drought stress conditions.Moreover,multiple carbon metabolites including amino acids,organic acids,sugars,sugar alcohols,and aromatic amines were also positively regulated by AtPED2.Taken together,these results indicated a positive role for AtPED2 in drought resistance,through modulation of stress-responsive genes expression,ROS metabolism,and metabolic homeostasis,at least partially. | Haitao Shi Tiantian Ye Fan Yang Zhulong Chan | 2015 | Journal of Integrative Plant Biology2015，57，9： | 0 |
| 12 | 枳橙四倍体与二倍体干旱生理应答及水通道蛋白基因表达分析显示文摘选盆栽2年生植株为试材,采用叶片离体脱水试验,自然干旱处理后形态、生理应答及水通道蛋白基因表达比较分析,对枳橙[Citrus sinensis(L.)Osb.×Poncirus trifoliata(L.)Raf.]二倍体及其同源四倍体的干旱胁迫耐受性进行评价。结果表明,离体脱水条件下,四倍体叶片相对失水率低于二倍体;自然干旱胁迫条件下,二倍体叶片萎蔫、黄化更严重;随着干旱胁迫的进行,两种倍性叶片丙二醛(MDA)、脯氨酸(Pro)含量及相对电导率都呈上升趋势,四倍体的MDA含量和相对电导率始终低于二倍体,Pro含量则始终高于二倍体;叶片超氧化物歧化酶(SOD)和过氧化物酶(POD)活性在干旱处理中表现为先升后降,四倍体的SOD及POD活性总体高于二倍体;在干旱15d时,在测定的9个水通道蛋白基因中,除PtrNIP1;1相对表达量是四倍体低于二倍体外,其余基因(PtrPIP1;2、PtrPIP1;3、PtrPIP2;2、PtrNIP1;2、PtrTIP1;3、PtrTIP4;1、PtrSIP1;1、PtrSIP1;2)的相对表达量均是四倍体高于二倍体。枳橙四倍体相比二倍体具有更强的耐干旱能力,与其干旱胁迫下的生理响应及水通道蛋白基因表达发生变化有关。 | 彭滢 李晓妍 陈芝琳 彭婷 钟八莲 肖璇 | 2021 | 中国南方果树2021，50，4： | 0 |
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