共被期刊论文引用了2次
您的检索式：您选中1篇文献正在查看引证文献汇总
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | NORHA,a novel follicular atresia-related lncRNA,promotes porcine granulosa cell apoptosis via the miR-183-96-182 cluster and FoxO1 axis显示文摘Background:Follicular atresia has been shown to be strongly associated with a low follicle utilization rate and female infertility,which are regulated by many factors such as microRNAs(miRNAs),which constitute a class of noncoding RNAs(ncRNAs).However,little is known about long noncoding RNAs(lncRNAs),which constitute another ncRNA family that regulate follicular atresia.Results:A total of 77 differentially expressed lncRNAs,including 67 upregulated and 10 downregulated lncRNAs,were identified in early atretic follicles compared to healthy follicles by RNA-Sequencing.We characterized a noncoding RNA that was highly expressed in atretic follicles(NORHA).As an intergenic lncRNA,NORHA was one of the upregulated lncRNAs identified in the atretic follicles.To determine NORHA function,RT-PCR,flow cytometry and western blotting were performed,and the results showed that NORHA was involved in follicular atresia by influencing GC apoptosis with or without oxidative stress.To determine the mechanism of action,bioinformatics analysis,luciferase reporter assay and RNA immunoprecipitation assay were performed,and the results showed that NORHA acted as a‘sponge’,that directly bound to the miR-183-96-182 cluster,and thus prevented its targeted inhibition of FoxO1,a major sensor and effector of oxidative stress.Conclusions:We provide a comprehensive perspective of lncRNA regulation of follicular atresia,and demonstrate that NORHA,a novel lncRNA related to follicular atresia,induces GC apoptosis by influencing the activities of the miR-183-96-182 cluster and FoxO1 axis. | Wang Yao Zengxiang Pan Xing Du Jinbi Zhang Honglin Liu Qifa Li | 2022 | Journal of Animal Science and Biotechnology2022，13，2： | 2 |
| 2 | 哺乳动物卵泡发育过程中组蛋白甲基化修饰的研究进展显示文摘卵泡的正常发育涉及有序的基因转录激活和抑制等一系列复杂的生命过程,对雌性获得生殖能力至关重要。组蛋白甲基化修饰可以改变细胞内染色质的状态,影响基因的转录活性。现阶段的研究表明,组蛋白甲基化等表观遗传学修饰在雌性哺乳动物卵泡发育的过程中发挥了重要的调控作用。本文总结了组蛋白赖氨酸甲基化(H3K4及H3K9)等甲基化修饰与生殖细胞发育的关系及作用机制,包括其在卵泡发育过程中的有序动态变化规律,以及H3K4me3等通过结合在不同基因启动子区来调控其表达,进而影响生殖细胞表观遗传学重编程、卵母细胞转录和减数分裂等过程的进展。本综述将为开展与组蛋白甲基化修饰与性腺中实质细胞发育和成熟相关机制研究提供参考。 | 张春娇 王超 | 2021 | 生理学报2021，73，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员