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| 1 | 东帕米尔高原喜马拉雅雪鸡遗传多样性及系统发育地位显示文摘东帕米尔高原作为生物多样性丰富的区域之一,喜马拉雅雪鸡和藏雪鸡在此混群分布。以东帕米尔高原喜马拉雅雪鸡为研究对象,采用PCR和测序技术,研究了mt DNA D-loop区序列特征,下载Gen Bank已提交的雪鸡序列,利用最大似然法构建系统发育树和中介网络关系,以阐明东帕米尔高原喜马拉雅雪鸡遗传多样性水平和系统进化地位。结果表明:东帕米尔高原喜马拉雅雪鸡mt DNA D-loop序列富含A、T碱基,含量为59.8%,存在64个多态位点,占核苷酸总数的5.5%,其中单一多态位点29个,简约信息位点33个,两处插入或缺失,转换发生的频率远远高于颠换;25个个体存在23种单倍型,平均单倍型多样度(Hd)为0.92±0.0001,平均核苷酸多样度(π)为0.00958,平均核苷酸差异度(K)为11.067,说明东帕米尔高原喜马拉雅雪鸡核苷酸多样性较低,单倍型多样性高,具有较为丰富的遗传多样性;系统发育分析显示喜马拉雅雪鸡与藏雪鸡分为明显的两大簇群,本研究涉及的东帕米尔高原喜马拉雅雪鸡出现遗传分化,呈现明显的2个进化支;中介网络关系分析显示雪鸡具有明显的地理分布特征,本研究雪鸡84%的单倍型聚在以东帕米尔高原为中心的进化支上。因此,建议扩大塔什库尔干野生动物自然保护区(位于东帕米尔高原境内)范围,建立国家级自然保护区,恢复生态环境,以提高雪鸡栖息地的生存适宜性。 | 王玉涛 潘建飞 黄翠翠 胡平 韩建林 | 2018 | 生态学报2018，38，1： | 6 |
| 2 | Comparative phylogeography of two sister species of snowcock: impacts of species-specific altitude preference and life history显示文摘Background: Phylogeographical patterns and population dynamics are usually interpreted by environmental disturbances and geographic barriers of the past. However, sister species may exhibit disparate patterns of genetic structures and population dynamics due to their habitat preference and altitude segregation. In this study, we tested how species-specific altitude habitat affected phylogeographical patterns in two sister snowcock species, Tibetan(Tetraogallus tibetanus) and Himalayan Snowcocks(T. himalayensis).Methods: A panel of seven microsatellite loci and a fragment of Mitochondrial DNA Control Region were used to investigate genetic structures and population dynamics in hope of revealing the underlying evolutionary processes through the identification of possible past demographic events.Results: Our results suggest that T. himalayensis showed a significant phylogeographical signal in mtDNA(FST microsatellite(F the glacial-interglacial cycles in the = 0.66, p < 0.001) and ST Pleistocene and followed demog= 0.11, p < 0.001) data and is stable duringraphic contraction until 0.003 million years(Mys) ago. The phylogeographical signal of T. tibetanus is lower than the level of genetic difference among populations in mtDNA(FST icrosatellite(F= 0.41, p < 0.001) and mST s been experien= 0.09, p < 0.001) data, likely benefiting from stable habitats over a long period of time. T. tibetanus hacing expansion since 0.09 Mys ago. However, an abnormally haplotype H9 from T. himalayensis clustering with T. tibetanus was spotted.Conclusion: Our results indicate that differences in habitat preference and altitude specialities were reflected in the genetic structure patterns and population dynamics of these two species. These dissimilarities in life history traits might have affected the dispersal and survival abilities of these two species differently during environmental fluctuations. The results of this study also enriched our knowledge on population differentiation and connectivity in high altitude mountain ecosystems. | Bei An Lixun Zhang Yutao Wang Sen Song | 2020 | Avian Research2020，11，1： | 1 |
| 3 | 东帕米尔高原喜马拉雅雪鸡线粒体基因组序列测定与分析显示文摘喜马拉雅雪鸡属国家二级保护动物,是生存海拔最高的鸡类,同时在维持生态平衡和鸟类高海拔适应性研究中具有重要的科研价值。本研究以东帕米尔高原喜马拉雅雪鸡为研究对象,采用直接测序法,拼接组装获得该物种线粒体(mtDNA)全基因组序列,对mtDNA全序列进行注释和结构分析,并选择GenBank已提交的鸡形目鸟类mtDNA序列,利用邻接法构建系统发育树,为该物种的有效保护和开发利用提供分子水平的依据。结果表明:喜马拉雅雪鸡的mtDNA全长为16691 bp,基因种类、数量及排列顺序与鸟类线粒体基因典型排列顺序一致。基因组核苷酸含量为A:30.2%;T:23.9%;C:32.2%;G:13.8%;(A+T)含量(54%)略高于G+C(46%)含量,AT-skew为0.1167,有一定的碱基偏好。在本研究中,东帕米尔高原喜马拉雅雪鸡起始密码子以ATG为主,终止密码子以TAA为主。预测了22个tRNA的二级结构,均能形成典型的三叶草结构,共有错配25处,以GU错配为主,喜马拉雅雪鸡tRNASer(UCN)基因比藏雪鸡的tRNASer(UCN)基因多出3个碱基;系统发育树构建表明雪鸡与鹌鹑关系最近,我们发现东帕米尔高原喜马拉雅雪鸡与甘肃阿克塞自治县喜马拉雅雪鸡形成两个独立的进化支。 | 李静 潘建飞 何子璐 王玉涛 | 2020 | 基因组学与应用生物学2020，39，5： | 1 |
| 4 | Population structure and demographic history of the chukar partridge Alectoris chukar in China显示文摘 | Sen SONG Shijie BAO Ying WANG Xinkang BAO Bei AN Xiaoli WANG Naifa LIU | 2013 | Current Zoology2013，59，4： | 0 |
| 5 | 基于线粒体COX1基因探讨夜鹭的分歧时间显示文摘物种分化过程与原因是进化生物学和生态学研究的热点问题之一,分化的本质为基因的分化。研究采用全球分布的夜鹭作为研究对象,基于线粒体COX1基因,分别采用贝叶斯法和最大似然法构建其系统发育树;采用放松分子钟的方法,利用鹳形目与鹈形目的分歧时间结点68.92百万年,与企鹅目的分歧时间67.12百万年,及鹈形目与企鹅目的分歧时间72.25百万年作为校正点,使用BEAST软件构建其分歧时间树,目的是利用分子手段探究夜鹭的分歧时间,并尝试推测其可能的分化因素。结果表明:夜鹭可依分布区域而分为两个支系,亚洲支系和美洲支系;距今56.74百万年左右,两支系分开;分歧原因可能与亚洲大陆和美洲大陆的分离有关。 | 王野影 粟海军 熊勇 张明明 | 2019 | 生物学杂志2019，36，3： | 0 |
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