共被期刊论文引用了5次
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| 1 | 甲基丙烯酸环氧丙酯与DNA的结合作用显示文摘运用吸收光谱移动法观察到甲基丙烯酸环氧丙酯Glycidyl Mcthacrylate,GMA与哺乳类基因组DNA具有强的结合作用。GMA浓度在0—2.8mmol/L范围内,DNA的吸收光谱随GMA浓度的增加而增加了向长波方向位移,并伴随吸收强度降低。若固定GMA浓度,改变DNA浓度,GMA吸收光谱也出现与上类似现象。实验还表明GMA与DNA的结合键型为共价型。 | 谢大英 张伟 孙文庆 高惠兰 李忠生 许建宁 方福德 | 1992 | 癌变.畸变.突变1992，4，5： | 8 |
| 2 | Genotoxic and Nongenotoxic Effects of Glycidyl Methacrylate on Human Lung Fibroblast Cells显示文摘To evaluate the genotoxic and nongenotoxic effects of short-term exposure to glycidyl mathacrylate (GMA) on human lung fibroblast cells (2BS cells) in vitro. Methods DNA strand breakage was determined by single cell gel electrophoresis, and DNA ladder formation assay and flow cytometric analysis were carried out to detect apoptic responses of cells to GMA exposure.The HPRT gene mutation assay was used to evaluate the mutagenicity, and the effect of GMA on gap junctional intercellular communication (GJIC) in the exposed cells was examined with the scrape loading/dye transfer technique. The ability of GMA to transform 2BS cells was also tested by an in vitro cell transformation assay. Results Exposure to GMA resulted in a dose-dependent increase in DNA strand breaks but not apoptic responses. GMA was also shown to significantly induce HPRT gene mutations and morphological transformation in 2BS cells in vitro. In contrast, GMA produced a concentration-dependent inhibition of GJIC. Conclusions GMA elicits both genotoxic and nongenotoxic effects on 2BS cells in vitro. The induction of DNA damage and gene mutations and inhibition of GJIC by GMA may casually contribute to GMA-induced cell transformation. | XUE-JUNYIN FU-DEFANG JIAN-NINGXU CHANG-QIZOU FENG-SHENGHE | 2003 | Biomedical and Environmental Sciences2003，16，3： | 5 |
| 3 | Prevention of chemical carcinogenesis using glutathine S-transferase-pi (GST-pi)显示文摘In order to explore whether the protective function of GST-pi can prevent transformation in vitro, NIH3T3 cells and carcinogen glycidyl methacrylate (GMA) have been used in cell transformation study. NIH3T3 cells have been transfected with GST-pi cDNA inserted retrovirus vector, pXT1, and then G418 resistant clones have been analyzed by Southern and Northern analyses. NIH3T3/pXGST clones that stably express GST-pi and control cells, | Minlei Zhang Fude Fang | 1999 | Chinese Science Bulletin1999，44，23： | 0 |
| 4 | 双链DNA直接测序法在环境诱变剂致突变分子机制研究中的应用Ⅱ.甲基丙烯酸环氧丙酯诱导质粒抗性基因突变的测定显示文摘运用我所建立的双链DNA直接测序法测定了GMA诱导的质粒抗性基因片段(Tc^R)EcnRI/SaII片段(~0.3Kb)的序列,并与相应正常基因片段序列作一比较,所得结果具有一定意义。本实验表明:双链DNA直接测序法可应用于环境诱变剂研究。 | 高惠兰 谢大英 左瑾 方福德 | 1993 | 癌变.畸变.突变1993，5，1： | 0 |
| 5 | 分子生物学技术在遗传毒理学中的应用显示文摘遗传毒理学是一门年轻的分支学科,仅为20多年的发展历史。在过去的20几年中,正逢分子生物学鼎盛发展时期,她以DNA重组技术所获得的巨大成就有力地促进了生命科学各学科之间的相互渗透和交流,从而使研究推向分子水平并逐步深入。遗传毒理学亦不例外,她已经并正在继续借鉴和应用分子生物学理论和技术来解决传统遗传毒理学难以解决的问题,这里拟简要介绍一些主要的情况。 | 方福德 高惠兰 | 1993 | 癌变.畸变.突变1993，5，1： | 0 |
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