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| 1 | High frequencies of HGV and TTV infections in blood donors in Hangzhou显示文摘ALM To determine the frequencies of HGV and TTVinfections in blood donors in Hangzhou.METHODS RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed.``RESULTS Thirty-two ( 15.8%) and 30 (14.8%) of the 203serum samples were positive for HGV RNA and TTV DNA,respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA.Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3serum samples compared with the reported HGV and TTV sequences was 89.36%. 87.94%, 88.65% and 63.51%.65.77% and 67.12%. respectively.``CONCLUSION The infection rates of HGV and/or TTV inblood donors are relatively high. and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas. | Jie Yan~1 Li-Li Chen~2 Yi-Hui Luo~1 Ya-Fei Mao~1 Meng He~1 1 Department of Pathogenic Biology,Medical School of Zhejiang University,Hangzhou 310006,Zhejiang Province,China2 Department of Stomatology,The Second Affiliated Hospital,Medical School of Zhejiang University,Hangzhou 310009,Zhejiang Province,China | 2001 | World Journal of Gastroenterology2001，7，5： | 11 |
| 2 | 长春地区TTV感染与其他病毒性肝病关系的流行病学调查显示文摘目的了解长春地区TTV的感染状况方法采用酶联免疫吸附实验,对前来白求恩医科大学第三临床学院消化内科就诊或住院的213例长春地区患者进行抗-TTVIgG的检测,并进一步分析TTV感染情况与性别、年龄、传播途径的关系,探讨TTV的致病性以及TTV感染与其他病毒性肝病的关系。结果长春地区存在TTV感染者,感染率为7.98%;从感染与性别、年龄无关;TTV可经胃肠道和胃肠道外两种途径而传播;TTV感染可见于急性肝炎、慢性肝炎、肝硬变和肝癌患者,但也可表现为单纯携带;TTV可与乙型肝炎病毒(HBV)、丙型肝炎病毒(HCV)合并存在,但合并HCV感染的概率要明显高于合并HBV感染的概率。不过,TTV感染并不加重HBV,HCV对肝脏的损害,与HBV,HCV也无协同致病作用。结论长春地区存在TTV感染者,TTV感染可能与丙型肝炎病毒感染有一定联系。 | 赵岩 王江滨 | 2001 | 世界华人消化杂志2001，9，7： | 10 |
| 3 | Transfusion transmitted virus infection in general populations and patients with various liver diseases in south China显示文摘INTRODUCTIONAlthough several specific detecting methods hadbeen applied to determine the hepatitis virus,therewas a lot of cryptogenic hepatitis without anyknown hepatitis infectious marker.Theprevalence of hepatitis G virus (HGV) (also knownas GB-C virus) infection has been reported to be 5%-13% in patients with non-A-E hepatitis andcirrhosis,however,there is little evidencesuggesting that HGV causes hepatitis in human. | Chen YP Liang WF Zhang L He HT Luo KX | 2000 | World Journal of Gastroenterology2000，6，5： | 7 |
| 4 | Investigation of HGV and TTV infection in sera and saliva from non-hepatitis patients with oral diseases显示文摘AIM: To determine the frequencies of HGV and TTV infectionsin serum and saliva samples of non-hepatitis patients withoral diseases in Hangzhou area, and to understand thecorrelation between detected results of HGV RNA and/or TTVDNA in sera and in saliva from the same patientsMETHODS: RT-nested PCR for HGV RNA detection andsemi-nested PCR for nv DNA detection were performed inthe serum and saliva samples from 226 non-hepatitis patientswith oral diseases, and nucleotide sequence analysis.RESULTS: Twenty-seven (11.9 %) and 21 (9.3 %) of the226 serum samples were only positive for HGV RNA andlrv DNA, respectively. 10 (4.4 %) and 9 (3.9 %) of the226 saliva samples were only positive for HGV RNA andTTV DNA, respectively. And 7 (3.1%) of the serum samplesand 2 (0.9 %) of the saliva samples showed the positiveamplification results for both HGV RNA and Irv DNA. 12saliva samples from the 34 patients (35.3 %) with HGV orHGV/TTV viremia and 11 saliva samples from the 28 patients(39.3 %) with TTV or HGV/TTV viremia were HGV RNAdetectable, respectively, including two patients positive forboth HGV RNA and TTV DNA in serum and saliva samples.No saliva samples from the 226 patients were found to beHGV RNA or nv DNA detectable while their serum sampleswere negative for HGV or TTV. Homologies of the nucleotidesequences of HGV and TTV amplification products from theserum and saliva samples of the two patients comparedwith the reported sequences were 88.65-91.49 % and65.32-66.67 %, respectively. In comparison with thenucleotide sequences of amplification products betweenserum and from saliva sample from any one of the twopatients, the homologies were 98.58 % and 99.29 % forHGV, and were 98.65 % and 98.20 % for rTV, respectively.CONCLUSION: Relatively high carrying rates of HGV and/or TTV in the sera of non-hepatitis patients with oral diseasesin Hangzhou area are demonstrated. Parts of the carriersare HGV and/or TTV positive in their saliva. The results ofthis study indicate that dentists may be one of the populationswith high risk for HGV and/or TTV infection, and by way of saliva HGV and TTV may be transmitted among individuals. | Jie Yan Department of Pathogenic Biology,College of Medical Science,Zhejiang University,Hangzhou 310031,Zhejiang Province,China Li-Li Chen Department of Stomatology,The Second Affiliated Hospital,College of Medical Science,Zhejiang University,Hangzhou 310009,Zhejiang Province,China Yong-Liang Lou Xiao-Zhi Zhong Wenzhou Medical College,Wenzhou 325000,Zhejiang Province,China | 2002 | World Journal of Gastroenterology2002，8，5： | 6 |
| 5 | Clinicopathological study on TTV infection in hepatitis of unknown etiology显示文摘AIM: To investigate the state of infection, replication site,pathogenicity and clinical significance of transfusiontransmitted virus (TTV) in patients with hepatitis, especiallyin patients of unknown etiology.METHODS: Liver tissues taken from 136 cases of non-A non-G hepatitis were tested for Tr virus antigen and nucleic acidby in situ hybridization (ISH) and nested-polymerase chainreaction (PCR). Among them, TT virus genome and itscomplemental strand were also detected in 24 cases ofautopsy liver and extrahepatic tissues with ISH. Meanwhile,TTV DNA was detected in the sera of 187 hepatitis patientsby nested-PCR. The pathological and clinical data of thecases infected with nv only were analyzed.RESULTS: In liver, the total positive rate of rrv DNA was32.4 % and the positive signals were located in the nuclei ofhepatocytes. In serus, rrv DNA was detected in 21. 4 %cases of hepatitis A-G, 34.4 % of non-A non-G hepatitis and15 % of healthy donors. The correspondence rate of lrvDNA detection between liver tissue with ISH and sera withPCR was 63.2 % and 89.3 % in the same liver tissues by ISHand by PCR, respectively. Using double-strand probes andsingle-strand probes designed to detect TTV genome, thecorrespondence rate of Trv DNA detected in liver andextrahepatic tissues was 85. 7 %. Using single-strandprobes, TTV genome could he detected in liver andextrahepatic tissues by PCR, but its complemental strands(replication strands) could be observed only in livers. Theliver function of most cases infected with TTV alone wasabnormal and the liver tissues had different pathologicaldamage such es ballooning, acidophilia degeneration,formation of apoptosis bodies and focus of necrosis, but the inflammation in the Iobule and portal area was mild.CONCLUSION: The positive rate of TTV DNA among cases ofhepatitis was higher than that of donors, especially inpatients with nonA non-G hepatitis, but most of them werecoinfected with other hepatitis viruses. Lrv can infect notonly hepatocytes, but also extrahepstic tissues. However,the chief replication place may be liver. The infection of lrvmay have some pathogenicity. Although the pathogenicity iscomparatively weak, it can still damage the liver tissues.The lesions in acute hepatitis (AH) and chronic hepatitis(CH) am mild, but in severe hepatitis (SH) , it can be veryserious and cause liver function failure, therefore, we shouldpay more attention to TTV when studying the possiblepathogens of so-called 'liver hepatitis of unknown etiology'. | Zhong-Jie Hu Zhen-Wei Lang Yu-Sen Zhou Hui-Ping Yan De-Zhuang Huang Wan-Rong Chen Zhao-Xia Luo,Department of Pathology,Beijing You’an Hospital,Beijing 100054,China | 2002 | World Journal of Gastroenterology2002，8，2： | 6 |
| 6 | 非肝炎口腔科患者血清及唾液中HGV和TTV感染率的调查显示文摘目的 检测杭州地区非肝炎口腔科患者血清和唾液标本中庚型肝炎病毒 (HGV)和输血传播病毒 (TTV)感染率 ,进一步了解HGV和TTV传播途径和感染的高危人群。方法 分别从上述患者血液和唾液标本中提取RNA和DNA ,采用逆转录 -套式聚合酶链反应 (RT -NestedPCR)和半套式聚合酶链反应 (Hemi-nestedPCR)分别检测HGV5’ -NCRRNA和TTVN2 2区DNA ,部分HGV和TTV扩增产物克隆后进行核苷酸序列测定。结果 2 2 6例血清标本中 ,仅HGV阳性 2 7例(11 9% )、仅TTV阳性 2 1例 (9 3% )、HGV和TTV均阳性 7例 (3 1% ) ;2 2 6例唾液标本中 ,仅HGV阳性 10例 (4 4 % )、仅TTV阳性 9例 (3 9% )、HGV和TTV均阳性 2例 (0 9% ) ;未见血清标本HGV和 /或TTV检测结果阴性而唾液标本阳性者。2例患者血清标本及唾液标本HGV和TTV均阳性的扩增产物分别与报道的HGV和TTV核苷酸序列比较 ,同源性为88 6 5 %~ 91 4 9%和 6 5 32 %~ 6 6 6 7% ,但各自的血清标本与唾液标本HGV和TTV扩增产物同源性分别高达 98 5 8%~99 2 9%和 98 6 5 %~ 98 2 0 %。结论 杭州地区非肝炎口腔科患者HGV或TTV感染率均较高 ,其中部分携带病毒的患者唾液中均可检出HGV或TTV ,提示HGV或TTV可能存在唾液传播途径 。 | 钟晓芝 严杰 毛亚飞 李立伟 李淑萍 罗依惠 | 2003 | 中国人兽共患病杂志2003，19，3： | 2 |
| 7 | 不同人群输血传播病毒感染的检测和传播途径分析显示文摘目的了解深圳地区不同人群TTV感染状况,探讨TTV传播途径。方法建立套式聚合酶链反应方法,对临床病毒性肝炎病人、血液透析病人、静脉吸毒者、性病患者和献血者人群进行TTVDNA的PCR检测,并对部分阳性株进行序列测定。结果非甲~戊型肝炎病人、血透患者和反复输血病人TTVDNA阳性率分别为41.7%、40.5%和35.7%,明显高于甲~丙型肝炎病人(17.7%,P<0.01);静脉吸毒者中TTVDNA阳性率高达39.3%,明显高于献血者人群(20.4%,P<0.05);ALT异常献血员中TTVDNA检出率为明显高于无偿献血员人群(P<0.05);性病患者生殖道分泌物中TTVDNA检测出率为15.8%。2株TTV分离株与TTV标准株(AB008394)同源性在92.5%以上。结论TTV感染与肝炎有关,可能是非甲~庚型肝炎的病原之一;TTV除了经血源途径传播外,性传播可能成为重要传播途径之一。 | 江鹏飞 黄呈辉 熊海燕 欧阳玲 张寿斌 | 2006 | 江西医药2006，41，2： | 0 |
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