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1Plant pleiotropic drug resistance transporters:Transport mechanism, gene expression,and function显示文摘Pleiotropic drug resistance(PDR) transporters belonging to the ABCG subfamily of ATP-binding cassette(ABC)transporters are identified only in fungi and plants. Members of this family are expressed in plants in response to various biotic and abiotic stresses and transport a diverse array of molecules across membranes. Although their detailed transport mechanism is largely unknown, they play important roles in detoxification processes, preventing water loss, transport of phytohormones,and secondary metabolites. This review provides insights into transport mechanisms of plant PDR transporters, their expression profiles, and multitude functions in plants.Mohammed Nuruzzaman Ru Zhang Hong-Zhe Cao Zhi-Yong Luo 2014Journal of Integrative Plant Biology2014,56,8:15
2Auxin biosynthesis by the YUCCA6 flavin monooxygenase gene in woodland strawberry显示文摘Auxin has been regarded as the main signal molecule coordinating the growth and ripening of fruits in strawberry,the reference genomic system for Rosaceae.The mechanisms regulating auxin biosynthesis in strawberry are largely elusive.Recently,we demonstrated that two YUCCA genes are involved in flower and fruit development in cultivated strawberry.Here,we show that the woodland strawberry(Fragaria vesca L.)genome harbors nine loci for YUCCA genes and eight of them encode functional proteins.Transcription pattern in different plant organs was different for all eight FvYUCs.Functionality of the FvYUC6 gene was studied in transgenic strawberry overexpressing FvYUC6,which showed typical high-auxin phenotypes.Overexpression of FvYUC6 also delayed flowering and led to complete male sterility in F.vesca.Additionally,specific repression of FvYUC6 expression by RNA interference significantly inhibited vegetative growth and reduced plant fertility.The development of leaves,roots,flowers,and fruits was greatlyaffected in FvYUC6-repressed plants.Expression of a subset of auxin-responsive genes was well correlated with the changes of FvYUC6 transcript levels and free indole-3-acetic acid levels in transgenic strawberry.These observations are consistent with an important role of FvYUC6 in auxin synthesis,and support a main role of the gene product in vegetative and reproductive development in woodland strawberry.Hong Liu Wei-Fa Xie Ling Zhang Victoriano Valpuesta Zheng-Wen Ye Qing-Hua Gao Ke Duan 2014Journal of Integrative Plant Biology2014,56,4:4
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