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| 1 | 国产耳草属长节耳草及其相似种的分类研究显示文摘我国耳草属中具有茎四棱和头状花序特征的植物常常被鉴定为长节耳草(Hedyotis uncinella),并且这一名称还包括了基于不同模式的3个分类学异名。由于这一类植物在叶片形态、叶柄长短、花序着生式样以及花梗长度等形态特征方面存在着明显的不同,因此将这一类植物笼统地鉴定为长节耳草并不合理。为清晰区分这类植物,通过模式比对、居群形态特征变异式样的野外观察以及分子系统学分析等方法进行研究,结果表明,以前被鉴定为“长节耳草”的植物实际上包括了6个分类实体,即被归并的丰花耳草(H.borrerioides)、垦丁耳草(H.kuraruensis)和新组合种——团花耳草(H.cephalophora)均应独立成种,而长节耳草本种、被忽略的线叶耳草(H.linearifolia)和我国新记录种——球花耳草(H.multiglomerulata)各自也应得到承认。同时,为了便于进行分类鉴定,也提供了这些种类的分种检索表。 | 袁明灯 江国彬 王瑞江 | 2022 | 热带亚热带植物学报2022，30，3： | 0 |
| 2 | Identification of Andrographis Herba and its common products using mini-barcode显示文摘Andrographis Herba,the aerial part of Andrographis paniculata(Burm.f.)Wall.ex Nees(Acanthaceae),has a wide geographic distribution and has been used for the treatment of fever,cold,inflammation,and other infectious diseases.In markets,sellers and buyers commonly inadvertently confuse with related species.In addition,most Chinese medicinal herbs are subjected to traditional processing procedures,such as steaming and boiling,before they are sold at dispensaries;therefore,it is very difficult to identify Andrographis Herba when it is processed into Chinese medicines.The identification of species and processed medicinal materials is a growing issue in the marketplace.However,conventional methods of identification have limitations,while DNA barcoding has received considerable attention as a new potential means to identify species and processed medicinal materials.In this study,17 standard reference materials of A.paniculata,2 standard decoctions,27 commercial products and two adulterants were collected.Based on the ITS2 sequence,it could successfully identify A.paniculata and adulterants.Moreover,a nucleotide signature consisting of 71 bp was designed,this sequence is highly conserved and specific within A.paniculata while divergent among other species.Then,we used these new primers to amplify the nucleotide signature region from processed materials.In conclusion,the DNA barcoding method developed in the present study for authenticating A.paniculata is rapid and cost-effective.It can be used in the future to guarantee the quality of Andrographis Herba of each regulatory link for clinical use. | XIONG Chao HUANG Chen-Hao WU Lan XU Ran XUE Jian-Ping LIU Zhi-Guo SUN Wei | 2022 | Chinese Journal of Natural Medicines2022，20，5： | 0 |
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